Gilland, Edwin, Robert Baker, and Winfried Denk. 2003. Long duration three-dimensional imaging of calcium waves in zebrafish using multiphoton fluorescence microscopy. Biol. Bull. 205:176-177.

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Single plane (movie 1) and multiple plane (movie 2) imaging sequences showing periodic calcium waves in a zebrafish embryo at an early somite stage. The sequences span 195 minutes of development, and the playback speed is 40 frames per second, yielding a time-lapse compression of about 560x real time. The orientation of the embryo is shown in the frame on the right. Movie 1 is a time-lapse sequence from the same imaging plane as Fig. 1D and is the source of the time profiles in Fig. 1F and G. In movie 2, sequences from 6 separate imaging planes constituting a z-axis depth of 90 microns were added together with the Image Calculator function in ImageJ. By adding the pixel values from multiple imaging planes, the calcium dynamics occuring through an extended depth of tissue can be represented in a single graphic frame.