Microscopy and Optics Resources

Microscopy and Optics Resources

UNDER CONSTRUCTION…

Imaging ‘Phase Objects’

imaging of living cells…

‘unstained’, ‘un-labeled’, ‘tag-free’

using variations in the intrinsic properties, such as the refractive index, of the materials in the cell.

Phase objects may be completely transparent, but they change the phase of the light passing through them.  Birefringence is caused by materials that have an index of refraction that varies with the orientation of the polarization of the light passing through them, thus causing phase shifts that can be measured.  (Dichroism is a related phenomenon, in that the amount of absorption of light depends on the polarization orientation.)

Microscopy Imaging Modes for Resolving Phase Objects

Utilize Polarized Light

phase-contrast, DIC, polarization, bifrefringence

Birefringence

Dichroism