Laboratory of Michael Shribak

Michael ShribakmicroscopyMichael Shribak, Associate Scientist
Cellular Dynamics Program

Phone (508) 289-7242
Fax (508) 289-7579
E-Mail mshribak@mbl.edu
Address MBL, 7 MBL Street,  Woods Hole, MA 02543

GALLERY: Click image for full view.


RESEARCH INTERESTS:
My research lies in the biomedical optics, and employs a combination of optical, mechanical and software designs to create advanced light microscopes for studying the architectural dynamics in living cells. In particularly, we invented new types polarized light and differential interference microscopes, which non-invasively create contrast where structures are otherwise invisible. These new imaging modes allow to observe and record living cells with extremely fine details, some of which have never been seen with any other mode of microscopy before. Since image contrast in these modes is generated without the need to stain or to modify the cell in any way, cells can be followed for long periods of time non-invasively, as they undergo mitosis, organelle movement or transformation, morphogenesis, and other dynamic changes. Yet these imaging modes should be compatible with fluorescent staining if desired.

More specifically, my current research includes the following subjects:

  • Orientation-independent differential interference contrast (OI-DIC) microscopy for quantitative imaging refractive index (dry mass) distribution within thin optical section in living cells with high sensitivity, resolution and speed;
  • Orientation-independent differential polarized light (OI-Pol) microscopy for quantitative imaging birefringence (molecular orientation and mechanical stress) distribution within live cell with high sensitivity, resolution and speed;
  • Developing microscope setup, which rapidly switches between the OI-DIC and OI-Pol modes without any mechanical movement. The setup would provide images, which show complementary specimen features, dry mass and dry mass anisotropy correspondently. For instance, in a live dividing cell, the DIC image will clearly show the chromosomes while the OI-Pol image will depict the distribution of the birefringent microtubules in the spindle;
  • New type of true orientation-independent polarized light microscope technique for real-time birefringence imaging, which we called the Video-Enhanced Polychromatic polscope;
  • Polarized fluorescence microscopy;
  • Polarization aberration in lens systems, techniques for measurement and rectification of polarization aberration;
  • Interferometric technique for measuring two-dimensional dry mass distribution in living cells based on Jamin-Lebedeff and Mach-Zehnders microscopes.

Currently we are seeking collaboration with individuals interested in practical biological applications of the newly developed light microscopy techniques.

 

Complete Publication List (partially downloadable)

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Selected Publications:
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R.Oldenbourg, M.Shribak, “Microscopes”. In: Handbook of Optics, Third Edition, Volume I: Geometrical and Physical Optics, Polarized Light, Components and Instruments (ed. M.Bass), McGraw-Hill Professional, (2010).
M.Shribak, “Polarization”. In: Handbook of Optical Metrology: Principles and Applications (ed. T.Yoshizawa), CRC Press, Boca Raton, FL, USA, 339-350 (2009).
M.Shribak, “Orientation-independent differential interference contrast microscopy technique and device”, US Patents # 7564618 (PDF) and 7233434 (PDF) (2009).
M. Shribak, “Complete polarization state generator with one variable retarder and its application for fast and sensitive measuring of two-dimensional birefringence distribution”, The Journal of the Optical Society of America A, vol.28, No.3, 410-419 (2011). (PMCID: PMC3059986). (PDF)
M.Shribak, J.LaFountain, D.Biggs, S.Inoué, “Orientation-independent differential interference contrast (DIC) microscopy and its combination with orientation-independent polarization system”, Journal of Biomedical Optics, vol. 13, No.1, 14011 (2008). (selected for Virtual Journal of Biological Physics Research, vol.15, No.4 (2008)). (PDF)
M.Shribak, R.Oldenbourg, “Retardance measurement system and method”, US Patents #7372567 (PDF), 7239388 (PDF), and 7202950 (PDF) (2007). (licensed to CRi, Woburn, MA).
M.Shribak, S.Inoué, “Orientation-Independent Differential Interference Contrast Microscopy”, Applied Optics, 45, 460-469 (2006) (featured on cover page). (PDF)
M.Shribak, R.Oldenbourg, P.J.Cronin, C.C.Hoyt, and P.J.Miller “Instantaneous polarization measurement system and method”, US Patent #7079247 (2006) (licensed to CRi, Woburn, MA). (PDF)
M.Shribak, R.Oldenbourg, “Mapping polymer birefringence in three-dimensions using a polarization microscope with oblique illumination,” in Biophotonics Micro- and Nano-Imaging, D.Anselmetti, Ed., Proc. SPIE 5462, 57-67 (2004). (PDF)
M.Shribak, R.Oldenbourg, “Techniques for fast and sensitive measurements of two-dimensional birefringence distributions,” Applied Optics 42, 3009-3017 (2003). (PDF)
S.Inoué, O.Shimomura, M.Goda, M.Shribak, and P.T.Tran, “Fluorescent polarization of green fluorescent protein,” Proceedings of the National Academy of Sciences of the USA, 99, 4272 – 4277 (2002). (PDF)
M.Shribak, S.Inoué, and R.Oldenbourg, “Polarization aberrations caused by differential transmission and phase shift in high NA lenses: theory, measurement and rectification,” Optical Engineering, 41, 943-954 (2002). (PDF)
M.Shribak, Y.Otani, and T.Yoshizawa, “Autocollimation polarimeter for measuring two-dimensional distribution of birefringence,” Optics & Spectroscopy, 89, 155-159 (2000). (PDF)
M.I.Shribak “Autocollimating detectors of birefringence”, in International Conference on Optical Inspection and Micromeasurements, C. Gorecki, Ed., Proc. SPIE 2782, 805-813 (1996). (PDF)
M.I.Shribak, “Use of gyrotropic birefringent plate as quarter-wave plate”, Soviet Journal of Optical Technology, 53, 443-446 (1986). (PDF)

Complete Publication List