2023 OMIBS Schedule

DAY 1 - Monday, August 14, 2023

5:00 PM-6:00 PM

Swope

Walk around Woods Hole (optional; meet in front of Swope)

Malea Murphy, Abbi Sathapppan

6:00 PM-7:00 PM

Speck

Welcome and orientation

Alison North, Joerg Bewersdorf

7:00 PM-8:00 PM

Swope Terrace

Pizza party

8:00 PM-9:00 PM

Speck

Lecture: Grand overview of the course

Alison North

DAY 2 - Tuesday, August 15, 2023

8:15 AM-9:30 AM

Speck

Lecture: Foundations of microscopy 1 - geometrical optics

Joerg Bewersdorf

9:30 AM-10:00 AM

Break

10:00 AM-11:00 AM

Speck

Lecture: Foundations of microscopy 2 - lenses and optical systems

Joerg Bewersdorf

11:00 AM-12:00 PM

Loeb 2nd floor

Lab: Basic Sample preparation

Malea Murphy, Abbi Sathapppan, Michelle Itano, Alison North

12:00 PM-1:00 PM

Swope

Lunch

1:00 PM-3:15 PM

Loeb 2nd floor

Lab: Build basic microscopes

Mark Lessard, Jyot Antani

3:15 PM-3:30 PM

Loeb 2nd floor

Break

3:30 PM-6:30 PM

Loeb 2nd floor

Lab: Practice with commercial microscope stands

Jacqueline Leung + TAs

6:30 PM-7:30 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:30 PM-9:00 PM

Speck

Lecture: Foundations of microscopy 3 - wave optics

Joerg Bewersdorf

9:00 PM-10:00 PM

Loeb 2nd floor

Demonstration: Abbe diffraction apparatus

Chris Bjornsson

DAY 3 - Wednesday, August 16, 2023

8:15 AM-9:30 AM

Speck

Lecture: Transmitted light 1

Sharon King

9:30 AM-9:45 AM

Break

9:45 AM-11:15 AM

Speck

Lecture: Cameras

Stephanie Fullerton

11:15 AM-12:15 PM

Loeb 2nd floor

Lab: Phase contrast microscopy

Sharon King, Jacqueline Leung, TAs

12:15 PM-1:15 PM

Swope

Lunch

1:15 PM-3:15 PM

Loeb 2nd floor

Lab: ImageJ basics

Andrew McCall

3:15 PM-4:15 PM

Loeb 2nd floor

Lab: Use of cameras

Andrew McCall, Jyot Antani

4:15 PM-5:30 PM

Speck

Lecture: Transmitted light 2

Sharon King

5:30 PM-6:30 PM

Loeb 2nd floor

Lab: DIC and polarization (part 1)

Sharon King, Jacqueline Leung, TAs

6:30 PM-7:30 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:30 PM - 10:30 PM

DAY 4 - Thursday, August 17, 2023

8:15 AM-9:00 AM

Speck

Discussion of lab exercises

Sharon King

9:00 AM-9:45 AM

Speck

Lecture: Photophysics of fluorescence and dye chemistry

Luke Lavis

9:45 AM-10:45 AM

Speck

Lecture: Introduction to the fluorescence microscope

Alison North

10:45 AM-11:00 AM

Break

11:00 AM-12:30 PM

Loeb 2nd floor

Lab: Fluorescence microscopy (part 1)

Alison North, Michelle Itano, Jacqueline Leung

12:30 PM-1:30 PM

Swope

Lunch

1:30 PM-2:00 PM

Speck

Lecture: Colocalization

Alison North

2:00 PM-2:30 PM

Speck

Lecture: Instrumentation for live-cell imaging

Michelle Itano

2:30 PM-3:00 PM

Speck

Lecture: Fluorescence 2 - Chemical tags for functional imaging

Luke Lavis

3:00 PM-3:20 PM

Speck

Tech bites 1

Alison North, commercial faculty

3:20 PM-3:30 PM

Break

3:30 PM-4:00 PM

Loeb 2nd floor

Lab: Cleaning optical filters

Mark Lessard

4:00 PM-6:00 PM

Loeb 2nd floor

Lab: Fluorescence microscopy (part 2)

Alison North, Michelle Itano, Jacqueline Leung

6:00 PM-7:00 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:00 PM-9:30 PM

Loeb 2nd floor

Lab: Build widefield fluorescence microscope

Mark Lessard, Jyot Antani

9:30 PM-11:00 PM

Loeb 2nd floor

Commercial faculty appreciation event

DAY 5 - Friday, August 18, 2023

8:15 AM-8:30 AM

Speck

Discussion of lab exercises

Alison North, Joerg Bewersdorf

8:30 AM-9:30 AM

Speck

Lecture: Fluorescent proteins

Alison Tebo

9:30 AM-10:00 AM

Break

10:00 AM-11:00 AM

Speck

Lecture: Imaging dynamics (incl. FRET, FLIM, FCS)

Mathew Tantama

11:00 AM-12:00 PM

Speck

Lecture: Biosensors

Alison Tebo

12:00 PM-1:00 PM

Swope

Lunch

1:00 PM-3:15 PM

Loeb 2nd floor

Lab: FRET & FLIM

Alison Tebo, Mathew Tantama, Jacqueline Leung, TAs

3:15 PM-4:00 PM

Discussion of FRET & FLIM lab exercises

Alison Tebo, Jacqueline Leung, TAs

4:00 PM-5:00 PM

Speck

Lecture: Sample preparation techniques

Alison North

5:00 PM-5:15 PM

Break

5:15 PM-6:15 PM

Speck

Lecture: Principles of digital imaging 1

Patrick La Riviere

6:15 PM-7:15 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:15 PM-8:15 PM

Speck

Lecture: Principles of digital imaging 2

Patrick La Riviere

8:15 PM - 10:15 PM

Speck

Lab: Digital imaging

Patrick La Riviere, Matthew Parent

DAY 6 - Saturday, August 19, 2023

8:15 AM-9:00 AM

Speck

Discussion of lab exercises

Patrick La Riviere, Joerg Bewersdorf

9:00 AM-9:45 AM

Speck

Lecture: Evaluating optical performance using PSFs

Lynne Turnbull

9:45 AM-10:00 AM

Break

10:00 AM-12:00 PM

Loeb 2nd floor

Lab: Resolution measurements

Joerg Bewersdorf

12:00 PM-12:30 PM

Loeb courtyard

Group Photo

Mark Lessard

12:30 PM-1:00 PM

Lunch

1:00 PM-5:00 PM

Afternoon off

5:00 PM-6:00 PM

Speck

Lecture: Deconvolution

Sid Shaw

6:00 PM-7:00 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:00 PM-8:30 PM

Speck

Lecture: Confocal microscopy

Sid Shaw

8:30 PM-8:45 PM

Break

8:45 PM-9:45 PM

Speck

Discussion of lab exercises

Sid Shaw

DAY 7 - Sunday, August 20, 2023

8:15 AM-9:15 AM

Speck

Lecture: Multiphoton microscopy

Cristina Rodriguez

9:15 AM-12:15 PM

Loeb 2nd floor

Lab: Deconvolution, confocal, multiphoton (part 1)

Sid Shaw, Doug Richardson, Cristina Rodriguez, Jacqueline Leung

12:15 PM-1:15 PM

Swope

Lunch

1:15 PM-4:15 PM

Loeb 2nd floor

Lab: Deconvolution, confocal, multiphoton (part 2)

Sid Shaw, Doug Richardson, Cristina Rodriguez, Jacqueline Leung

4:15 PM-4:45 PM

Break

4:45 PM-5:15 PM

Speck

Lecture: ImageJ tutorial on quantitative analysis

Sid Shaw

5:15 PM-6:45 PM

Speck

Lab: Quantification & analysis (part 1)

Sid Shaw

6:45 PM-7:45 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:45 PM-8:45 PM

Speck

Lab: Quantification & analysis (part 2)

Sid Shaw

8:45 PM-10:00 PM

Speck

Discussion of Lab Exercises

Sid Shaw

DAY 8 - Monday, August 21, 2023

8:30 AM-9:30 AM

Speck

Lecture: Light sheet microscopy

Wes Legant

9:30 AM-10:30 AM

Speck

Lecture: Tissue clearing and Expansion Microscopy

Doug Richardson

10:30 AM-11:00 AM

Break

11:00 AM-12:00 PM

Speck

Lecture: Adaptive optics

Cristina Rodriguez

12:00 PM-1:00 PM

Swope

Lunch

1:00 PM-2:00 PM

Speck

Lecture: Super-resolution 1 - SIM methods

Alison North, Joerg Bewersdorf

2:00 PM-5:00 PM

Loeb 2nd floor

Lab: Light-sheet microscopes, SIM, iSIM (part 1)

Alison North, Mark Lessard, Abhishek Kumar

5:00 PM-5:10 PM

Speck

Lecture: Super-resolution 2 - pan Expansion Microscopy

Joerg Bewersdorf

5:10 PM-6:00 PM

Swope

Lecture: Super-resolution 3 - STED

Joerg Bewersdorf

6:00 PM-7:00 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:00 PM-9:00 PM

Loeb 2nd floor

Lab: Light-sheet microscopes, SIM, iSIM (part 2)

Alison North, Mark Lessard, Abhishek Kumar

9:00 PM-10:30 PM

Speck

Tech bites 2

Alison North, commercial faculty

DAY 9 - Tuesday, August 22, 2023

8:30 AM-9:30 AM

Speck

Lecture: Super-resolution 4 - Single-molecule localization techniques

Joerg Bewersdorf

9:30 AM-10:30 AM

Speck

Lecture: Spatial Omics

Jacqueline Leung

10:30 AM-12:00 PM

Loeb 2nd floor

Lab: STED & single-molecule localization

Mark Lessard

12:00 PM-1:00 PM

Swope

Lunch

1:00 PM-4:00 PM

Loeb 2nd floor

Lab: Rotations on specialized instruments/demos

Jacqueline Leung

4:00 PM-5:00 PM

Speck

Lecture: Pitfalls and artifacts in super-resolution

Alison North, Joerg Bewersdorf, Jacqueline Leung

5:00 PM-6:00 PM

Speck

Career Panel

Alison North, Joerg Bewersdorf

6:00 PM-7:00 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:00 PM-10:00 PM

Loeb 2nd floor

Lab: Time on instruments / demos (part 1)

Jacqueline Leung, Mark Lessard

DAY 10 - Wednesday, August 23, 2023

8:30 AM-12:00 PM

Speck

Lab: Time on instruments / demos (part 2)

Jacqueline Leung, Mark Lessard

12:00 PM-1:00 PM

Swope

Lunch

1:00 PM-2:00 PM

Speck

Discussion of super-resolution labs and critique of images

Alison North, Joerg Bewersdorf

2:00 PM-2:45 PM

Speck

Lecture: Ethics and good practices in microscopy

Michelle Itano

2:45 PM-3:45 PM

Speck

Lecture: Imaging live specimens without killing them

Binyam Mogessie

3:45 PM-4:45 PM

Discussion: choosing the right microscope for an application

Alison North, Joerg Bewersdorf

4:45 PM-5:00 PM

Break

5:00 PM-6:00 PM

Speck

Mapping the subcellular proteome using imaging

Emma Lundberg

6:00 PM-6:30 PM

Break

6:30 PM-9:00 PM

Swope Terrace

Banquet

DAY 11 - Thursday, August 24, 2023

Morning

Check-out after Breakfast