Course Date: March 11 – March 19, 2017
Deadline: January 20, 2017 | Apply here
Room and Board: TBD
Financial Assistance Available: Yes (through FASEB – see application form)
Director: Eduardo Rosa-Molinar, The University of Kansas
The Immunohistochemistry & Microscopy (IHCM) course consists of: Module 1 that focuses on immunohistochemistry (IHC), fluorescence and confocal light microscopy; and Module 2 that focuses on immunogold labeling and transmission and scanning electron microscopy.
In each module, participants will devote three and one-half days (~11 hours per day) to lectures, hands-on laboratory sessions, and interacting with and receiving feedback from experts.
Individuals may enroll for one or both modules.
Laboratory demonstrations, exercises, discussions, and trouble-shooting sessions focus on:
1) Principles underlying protein fixation in cells and tissues –participants will learn about tissue preparation, have multiple hands-on experiences, and learn how to trouble shoot problems;
2) Antigen retrieval –participants will learn how antigen retrieval can significantly improve detection of many antigens and how to trouble shoot problems;
3) Controls –participants will learn about, discuss, and use controls that support specificity of IHC and immunogold labeling results; discuss variances in antibody specificity and conditions that may generate inconsistent IHC staining and immunogold labeling; and learn to trouble shoot problems;
4) Strategies for detecting the presence of specific antigens in cells and tissues – participants will learn about and use antibodies labeled with different fluorescent labels and with combined fluorescent label and gold nanoparticle (i.e., FluoroNanogold™) probes; analyze labeling results with epi-fluorescence, confocal, and electron microscopies; and learn about and use antibody dilution;
5) Technologies that automate immunohistochemistry – participants will be introduced to and use automated immunohistochemistry and new technologies for immunogold labeling and gain insight into when and how new technologies will benefit their research;
6) Basic elements of light and fluorescence microscopies – participants will learn to use bright-field, epi-fluorescence, wide-field deconvolution, and confocal microscopies and gain insight into how to choose the correct imaging platform for samples;
7) Basic elements of transmission and scanning electron microscopies – participants will learn to use transmission electron and scanning electron microscopes (i.e., field emission scanning electron microscope) and gain insight into how to choose the correct imaging platform for samples; and
8) Rigor and reproducibility – participants will use examples/case studies to discuss the design of reproducible IHC and immunogold labeling experiments, the ethics of imaging, and acceptable practice for image capture, management, and the appropriate use of software (i.e., Photoshop).
This course is appropriate for beginning scientists and those with more advanced skills, including undergraduate and graduate students, laboratory technicians, postdoctoral researchers, and new and established faculty/clinicians seeking to expand their IHC and microscopy techniques and knowledge. Participants will be grouped appropriately. Registration is limited to thirty-six.
For travel-related and course registration expenses, students from groups underrepresented in science may apply to the Federation of American Societies of Experimental Biology, Maximizing Access to Research Careers (the application is here) for financial support.
Charles W. Frevert (Leader; Module 1), University of Washington School of Medicine
Paul Webster (Leader; Module 2), Oak Crest Institute of Science
Denis Baskin, University of Washington School of Medicine
Mark Clymer, Laxco Incorporated
Paul Goodwin, GE Healthcare
Stephen M. Hewitt, National Cancer Institute
Louie Kerr, Marine Biological Laboratory
Jim McIlvain, Carl Zeiss MicroImaging, LLC
Richard D. Powell, Nanoprobes Incorporated
Eduardo Rosa-Molinar, The University of Kansas
Jerry (Gerald) Sedgewick, Imaging and Analysis, LLC
Cindy Smith, Ted Pella Incorporated
William L. Stahl, University of Washington School of Medicine
Scott M. Tanner, Limestone College
2017 Course Teaching Assistants:
Martha A Delaney, University of Washington School of Medicine
Brian Johnson, University of Washington School of Medicine
Heather Shinogle-Decker, The University of Kansas
Prem S. Thapa-Chetri, The University of Kansas
Irma I. Torres-Vazquez, The University of Kansas
Noraida Martinez-Rivera, The University of Kansas
Andor, an Oxford Instrument Company
Bitplane, an Oxford Instrument Company
Carl Zeiss Microscopy LLC
Chroma Technology Corporation
Electron Microscopy Sciences
GE Healthcare Life Sciences
The Histochemical Society
Intelligent Imaging Innovations (3i)
Ted Pella Incorporated
Vector Laboratories, Inc.
Additional Course Supporters:
American Society of Investigative Pathology
Federation of American Societies of Experimental Biology (FASEB) Maximizing Access to Research Careers (MARC) Program
Imaging and Analysis, LLC
Journal of Histochemistry and Cytochemistry