Immunohistochemistry and Microscopy (IHCM)

Course Information

Course Date: March 10 – March 18, 2018

Extended Deadline: January 26, 2018 | Apply here

Director: Eduardo Rosa-Molinar, The University of Kansas

Course Description

This course covers fluorescence and chromogenic immunohistochemical methods for light microscopy and includes epi-fluorescence, spinning disk and laser-scanning confocal microscopy.

Participants will devote seven days (~11 hours per day) to lectures, hands-on laboratory and imaging sessions, and interacting with and receiving feedback from experts. We strongly recommend that participants bring samples and/or image data for trouble-shooting sessions.

Laboratory demonstrations, exercises, discussions, and trouble-shooting sessions focus on:

1) Principles underlying protein fixation in cells and tissues – participants will learn about tissue preparation, have multiple hands-on experiences, and learn how to trouble shoot problems;

2) Antigen retrieval – participants will learn how antigen retrieval can significantly improve detection of many antigens and how to trouble shoot problems;

3) Controls – participants will learn about, discuss, and use controls that support specificity of IHC labeling results; discuss variances in antibody specificity and conditions that may generate inconsistent IHC staining; and learn to trouble shoot problems;

4) Strategies for detecting the presence of specific antigens in cells and tissues – participants will learn about and use antibodies labeled with different fluorescent and chromogenic labels; analyze labeling results with light, epi-fluorescence, and confocal; and learn about and use antibody dilution;

5) Technologies that automate immunohistochemistry – participants will be introduced to and use automated immunohistochemistry and gain insight into when and how new technologies will benefit their research;

6) Basic elements of light and fluorescence microscopies – participants will learn to use light, epi-fluorescence, wide-field deconvolution, and confocal microscopies and gain insight into how to choose the correct imaging platform for samples;

7) Rigor and reproducibility – participants will use examples/case studies to discuss the design of reproducible IHC labeling experiments (i.e., FluoroFinder, https://fluorofinder.com/), the ethics of imaging, and acceptable practice for image capture, image management (i.e., SlideBook, https://www.intelligent-imaging.com/slidebook), and the appropriate use of software (i.e., Inkscape, https://inkscape.org/en/).

This course is appropriate for beginning scientists and those with more advanced skills, including undergraduate and graduate students, laboratory technicians, postdoctoral researchers, and new and established faculty/clinicians seeking to expand their IHC and microscopy techniques and knowledge. Participants will be grouped appropriately. Registration is limited to thirty-six.

For travel-related and course registration expenses, students from groups underrepresented in science may apply to the Federation of American Societies of Experimental Biology (FASEB), Maximizing Access to Research Careers (the application is here) for financial support.

2018 Course Faculty & Lecturers

Charles W. Frevert, University of Washington School of Medicine
Denis Baskin, University of Washington School of Medicine
Mark Clymer, Laxco Incorporated
Paul Goodwin, GE Healthcare
Stephen M. Hewitt, National Cancer Institute
Louie Kerr, Marine Biological Laboratory
Jim McIlvain, Carl Zeiss MicroImaging, LLC
Eduardo Rosa-Molinar, The University of Kansas
Jerry (Gerald) Sedgewick, Imaging and Analysis, LLC
Scott M. Tanner, Clemson University
Phillipa Timmins, Aurox Ltd

Course Teaching Assistants:

Brian Johnson, University of Washington School of Medicine
Heather Shinogle-Decker, The University of Kansas
Irma I. Torres-Vazquez, The University of Kansas

Course Coordinator:

Noraida Martinez-Rivera, The University of Kansas

Course Support

Andor, an Oxford Instrument Company
Aurox Ltd
Bitplane, an Oxford Instrument Company
Carl Zeiss Microscopy LLC
Chroma Technology Corporation
Electron Microscopy Sciences
FluoroFinder LLC
GE Healthcare Life Sciences
The Histochemical Society
Intelligent Imaging Innovations (3i)
Jackson Immunoresearch
Nanoprobes Incorporated
Vector Laboratories, Inc.

Additional Course Supporters:

American Society of Investigative Pathology
Federation of American Societies of Experimental Biology (FASEB) Maximizing Access to Research Careers (MARC) Program
Imaging and Analysis, LLC
Journal of Histochemistry and Cytochemistry
Laxco Incorporated
Leica Biosystems