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An intensive and comprehensive
laboratory-oriented course in cellular and molecular neurobiology
intended for predoctoral students, postdoctoral or clinical
researchers, and young investigators beginning independent research
careers. Limited to 12 students.
A hallmark of this course is the extensive lab work done in close
collaboration with expert faculty. The course is divided into three
sections: Electrophysiology, Imaging, and Molecular Neurobiology. These
are taught by separate groups of faculty, usually six in each section,
and with many guest lecturers. Each section begins with specific
training in core laboratory techniques; students then undertake one- to
two-week directed or independent projects using the methods they have
learned. Didactic lectures are combined with laboratory experience in
order to establish a strong conceptual foundation for each section. A
typical day has 3 hours of lecture and 10 hours of lab.
Electrophysiological methods focus on patch-clamp and sharp electrode
recordings, performed on neurons in a variety of preparations,
including tissue culture, brain slices, isolated squid synapses, rat
cochlea, or whole fish. Optical methods include calcium imaging,
confocal and 2-photon microscopy, videomicroscopy, and electron
microscopy. Molecular techniques emphasize the use of forward and
reverse genetics in diverse systems such as Drosophila, C. elegans,
zebrafish,
chick embryos, and primary cells in culture. The impact of
genetic manipulations are assayed by real time PCR, laser
microdissection, single cell PCR, in situ hybridization, and a
variety of immunotechniques in addition to incorporating
electrophysiological and imaging techniques.
The goal of the course is to emphasize the strengths of a
multidisciplinary approach for studying the function of the nervous
system at the cellular and molecular levels.
This course is supported with funds provided by
National
Institute
of Neurological Disorders and Stroke, NIH
Howard
Hughes Medical Institute
The Grass Foundation
International Brain Research Organization
2009 Faculty and Lecturers
Carlos Aizenman, Brown University
Ricardo Araneda, University of
Maryland
Maureen Barr, Rutgers University
Diana Bautista, University of
California, Berkeley
Francisco Bezanilla, University of
Chicago
JoAnn Buchanan, Stanford University
Ingrid Bureau, INSERM
Akira Chiba, University of Miami
Kathryn Commons, Children's Hospital/Harvard Medical
Matthew Dalva, University of Pennsylvania
Robert Darnell, The Rockefeller
University
Graeme Davis, University of
California, San Francisco
Karl Deisseroth, Stanford University
Elva Diaz, University of California, Davis
Fiona Doetsch, Columbia University
Leanne Godinho, Technical University
Munich
Carlota González Inchauspe, IFIBYNE
Kurt Haas, University of British
Columbia
Erik Jorgensen, University of Utah,
HHMI
Julie Kauer, Brown University
Jeff Lichtman, Harvard University
John Lipscombe, Brown University
Isabel Llano, CNRS
Roderick Mackinnon, The Rockefeller University, HHMI
Andres Maricq, University of Utah
Alain Marty, CNRS
Kristina Micheva, Stanford University
Thomas Misgeld, Technical University Munich
Louis Ptacek, University of
California, San Francisco, HHMI
Tom Reese, National Institutes of Health
William Roberts, University of Oregon
Edward Ruthazer, McGill University
Jon Sack, Protean Research
Peter Scheiffele, Biozentrum University Basel
Steven Siegelbaum, Columbia University
Stephen Smith, Stanford University
School of Medicine
Ben Strowbridge, Case Western Reserve University
Alice Ting, Massachusetts Institute of
Technology
Larry Trussell, Oregon Health &
Science University
Li-Huei Tsai, Massachusetts Institute
of Technology
Osvaldo Uchitel, IFIBYNE
Linda Van Aelst, Cold Spring Harbor
Laboratory
Monica Vetter, University of Utah
Sam Wang, Princeton University
Philip Washbourne, University of Oregon
Ryohei Yasuda, Duke University Medical Center
Gary Yellen, Harvard Medical School
Yi Zuo, University of California, Santa Cruz
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