November 24, 2014

Analytical & Quantitative Light Microscopy

aqlmCourse Date: April 29-May 8, 2015

Deadline: January 21, 2015 | Online Application Form

2014 Schedule (PDF format)

AQLM 2014 Poster

Directors: Jagesh Shah, Harvard Medical School/Brigham and Women’s Hospital; and Justin Taraska, NIH

A comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of video and digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field and confocal microscopes. This course is limited to 32 students.

Laboratory exercises, demonstrations, and discussions include: (1) geometrical and physical optics of microscope image formation including Abbe’s theory of the microscope and Fourier optics; (2) interaction of light and matter; (3) phase contrast polarization and interference microscopy for the nondestructive analysis of molecular and fine-structural organization in living cells; (4) fluorescence microscopy, quantification of fluorescence, and GFP; (5) principles and application of digital video imaging, recording, analysis, and display; (6) digital image processing and quantitative digital image deconvolution; (7) ratiometric measurement of intracellular ion concentrations; (8) confocal microscopy; and (9) new advances in light microscopy such as FRET, FLIM, TIRF, and patterned illumination.

The program is designed primarily for: (1) university faculty, professional researchers, postdoctoral fellows, and advanced graduate students in the life sciences who wish to expand their experience in microscopy and to understand the quantitative issues associated with analysis of data obtained with optical microscopes; (2) individuals well-grounded in the physical sciences, who wish to exploit microscopy techniques for analyzing dynamic fine-structural and chemical changes; and (3) industrial scientists and engineers interested in advancing the design of equipment and techniques involving video and digital microscopy.

Lectures are followed by small group laboratory sessions and demonstrations. As a result, students will have opportunities for extensive hands-on experience with state-of-the-art optical, electronic, and digital imaging equipment guided by an experienced staff from universities and industry.

Partial support is provided by:
Howard Hughes Medical Institute

2014 Course Faculty & Lecturers:

Allen, John, Florida State University
Cameron, Lisa, Dana-Farber Cancer Institute (Harvard Medical School)
Hodgson, Louis, Albert Einstein College of Medicine
Jaqaman, Khuloud, UT Southwestern Medical Center
Kumar, Abhishek, Yale University School of Medicine
Maddox, Paul, University of North Carolina, Chapel Hill
Murray, John, University of Pennsylvania
Oldenbourg, Rudolf, Marine Biological Laboratory
Ross, Jennifer, University of Massachusetts Amherst
Rottenfusser, Rudolf, Carl Zeiss MicroImaging LLC
Salmon, Edward (Ted), University of North Carolina, Chapel Hill
Simon, Sanford, The Rockefeller University
Sluder, Greenfield, University of Massachusetts Medical School
So, Peter, Massachusetts Institute of Technology
Sochacki, Kem, National Institutes of Health
Tam, Jenny, Massachusetts General Hospital
Tani, Tomomi, Marine Biological Laboratory
Williams, Elizabeth, The Rockefeller University
York, Andrew, National Institutes of Health