Immunohistochemistry and Microscopy (IHCM)

courses_ihcm

Course Date: March 11 – March 19, 2017

Deadline: January 20, 2017 | Apply here

Director: Eduardo Rosa-Molinar, The University of Kansas

Course Outline: The Immunohistochemistry & Microscopy (IHCM) course is divided into two modules; Module 1. 3.5 days and evenings [11 hours daily] of lectures, small breakout discussions with faculty and staff, and laboratory sessions with experts in the field of immunohistochemistry [IHC] and light [including fluorescence and confocal] microscopy; Module 2. 3.5 days and evenings (11 hours daily) of lectures, small breakout discussions with faculty and staff, and laboratory sessions with experts in the field of immunogold labeling and electron [including transmission and scanning] microscopy). Students can enroll for the individual modules or for the full seven days and evenings.

The laboratory demonstrations, exercises, discussions, and trouble-shooting sessions focus on:

1) Principles underlying the fixation of proteins in cells and tissues – fixation on and preparation of cells and tissue prior to IHC and immunogold labeling constitutes one of the most important processes affecting the success of IHC and immunogold labeling studies, students will learn about and have multiple hands-on experiences with tissue preparation; they will also learn how to trouble shoot problems;

2) Antigen retrieval – students will learn about and discuss how the detection of many antigens can be significantly improved by antigen retrieval; they will also learn how to trouble shoot problems;

3) Controls – IHC and immunogold labeling experiments must include positive and negative controls to support the validity of staining and identify experimental artifacts, students will learn about, discuss, and use controls that support the specificity of IHC and immunogold labeling results as well as the variances in antibody specificity and conditions that may generate inconsistent IHC staining and immunogold labeling results and lead to inaccurate interpretation and conclusions; they will also learn how to trouble shoot problems;

4) Strategies for detecting the presence of specific antigens in cells and tissues – students will learn about and use antibodies labeled with different fluorescent labels and with combined fluorescent label and gold nanoparticle (i.e., FluoroNanogold™) probes and analyze the results with epi-fluorescence, confocal, and electron microscopies; they will also learn and use antibody dilution;

5) Technologies that automate immunohistochemistry – to achieve a better understanding of when and how new technologies will benefit their research, students will be introduced to and use automated immunohistochemistry and new technologies for immunogold labeling;

6) Basic elements of light and fluorescence microscopies – to gain insight into how to choose the correct imaging platform for their samples, students will learn to use various imaging platforms, including bright-field, epi-fluorescence, wide-field deconvolution, and confocal microscopies;

7) Basic elements of transmission and scanning electron microscopies – to gain insight into how to choose the correct imaging platform for their samples, students will learn to use various imaging platforms and detectors, including transmission electron microscope and scanning electron microscope (i.e., field emission scanning electron microscope); and

8) Rigor and reproducibility – students will discuss the design of reproducible IHC and immunogold labeling experiments and the ethics of imaging and acceptable practice for image capture, management, and the appropriate use of software (i.e., Photoshop) one images and figures for publication.

This course is appropriate for undergraduate and graduate students, laboratory technicians, postdoctoral researchers, new and established faculty/clinicians seeking to expand their techniques and knowledge of IHC and microscopy. It is appropriate for beginning scientists and those with more advanced skills. Participants will be grouped appropriately. Registration is limited to thirty-six.

Students from groups underrepresented in science may apply for financial support for travel-related expenses and course registration from the Federation of American Societies of Experimental Biology, Maximizing Access to Research Careers (the application is here).

2017 Academic and Industry Course Leaders and Lecturers:

Charles W. Frevert (Leader; Module 1), University of Washington School of Medicine
Paul Webster (Leader; Module 2), Oak Crest Institute of Science
Denis Baskin, University of Washington School of Medicine
Mark Clymer, Laxco Incorporated
Paul Goodwin, GE Healthcare
Stephen M. Hewitt, National Cancer Institute
Louie Kerr, Marine Biological Laboratory
Jim McIlvain, Carl Zeiss MicroImaging, LLC
Richard D. Powell, Nanoprobes Incorporated
Eduardo Rosa-Molinar, The University of Kansas
Jerry (Gerald) Sedgewick, Imaging and Analysis, LLC
Cindy Smith, Ted Pella Incorporated
William L. Stahl, University of Washington School of Medicine
Scott M. Tanner, Limestone College

2017 Course Teaching Assistants:

Martha A Delaney, University of Washington School of Medicine
Brian Johnson, University of Washington School of Medicine
Heather Shinogle-Decker, The University of Kansas
Prem S. Thapa-Chetri, The University of Kansas
Irma I. Torres-Vazquez, The University of Kansas

Course Coordinator:

Noraida Martinez-Rivera, The University of Kansas

Course Founders and Supporters:

Andor, an Oxford Instrument Company
Bitplane, an Oxford Instrument Company
Carl Zeiss Microscopy LLC
Chroma Technology Corporation
Electron Microscopy Sciences
FluoroFinder LLC
GE Healthcare Life Sciences
The Histochemical Society
Intelligent Imaging Innovations (3i)
Jackson Immunoresearch
Nanoprobes Incorporated
RMC Boeckeler
Ted Pella Incorporated
Vector Laboratories, Inc.

Additional Course Supporters:

American Society of Investigative Pathology
Cytoskeleton Incorporated
Federation of American Societies of Experimental Biology (FASEB) Maximizing Access to Research Careers (MARC) Program
Imaging and Analysis, LLC
Journal of Histochemistry and Cytochemistry
Laxco Incorporated
Leica Biosystems