Immunohistochemistry and Microscopy (IHCM)


Course Date: March 12 – March 17, 2016

Extended Deadline: January 28, 2016 | Apply here

Directors: Eduardo Rosa-Molinar, The University of Kansas and Charles W. Frevert, University of Washington School of Medicine

Course Outline: The Immunohistochemistry & Microscopy (IHCM) course is four full days and evenings (11 hours daily) of lecture and laboratory sessions with experts in the field of immunohistochemistry (IHC) and microscopy. The IHCM course goal is to provide participants in-depth theory of and extensive hands-on experience with immunohistochemistry (IHC) techniques as well as theory and hands-on experience with a broad range of microscopic imaging techniques. The course emphasizes hands-on laboratory time and small breakout discussions with faculty and staff.

The laboratory demonstrations, exercises, discussions, and trouble-shooting sessions focus on: 1) Principles underlying the fixation of proteins in tissues; because fixation and preparation of tissue prior to IHC constitutes one of the most important processes affecting the success of IHC studies, students will learn about and have multiple hands-on experiences with tissue preparation; they will also learn how to trouble shoot problems; 2) Antigen retrieval; students will learn about and discuss how the detection of many antigens can be significantly improved by antigen retrieval; 3) Controls; because IHC experiments must include positive and negative controls to support the validity of staining and identify experimental artifacts, students will learn about, discuss, and use controls that support the specificity of IHC results as well as the variances in antibody specificity and conditions that may generate inconsistent immune-staining and lead to inaccurate conclusions; 4) Strategies for detecting the presence of specific antigens in cells; students will learn about and use antibodies labeled with different fluorescent probes and analyze the results with epi-fluorescence or confocal microscopies; they will also use antibody dilution studies; 5) Technologies that automate immunohistochemistry; to achieve a better understanding of when and how new technologies will benefit their research, students will be introduced to and use automated immunohistochemistry; 6) Basic elements of light and fluorescence microscopies; to gain insight into how to choose the correct imaging platform for their samples, students will learn to use various imaging platforms, including bright-field, epi-fluorescence, wide-field deconvolution, and confocal microscopies. Using images collected in the course as well as previously acquired images, students will also learn about and discuss ethics of imaging and acceptable practice for image capture and management and appropriate use of Photoshop to develop figures for publication; and 7) Troubleshooting; students will learn how to troubleshoot problems with immunohistochemistry and images will be an integral part of the course; troubleshooting sessions will be held daily.

This course is appropriate for undergraduate and graduate students, laboratory technicians, postdoctoral students, new and established faculty/clinicians seeking to expand their techniques and knowledge of IHC and microscopy. It is appropriate for beginning scientists and those with more advanced skills. Participants will be grouped appropriately. Registration is limited to thirty.

Students from groups underrepresented in science may apply for financial support for travel-related expenses and course registration (the application is here).

2015 Academic and Industry Course Faculty:

Denis Baskin, University of Washington School of Medicine
Charles Frevert, University of Washington School of Medicine
Matthew Gastinger, Bitplane, an Oxford Instrument Company
Paul Goodwin, GE Healthcare
Stephen M. Hewitt, National Cancer Institute
Jim McIlvain, Carl Zeiss MicroImaging, LLC
Mark Clymer, Datacolor
Eduardo Rosa-Molinar, The University of Kansas
Jerry (Gerald) Sedgewick, Imaging and Analysis, LLC
William L. Stahl, University of Washington School of Medicine

2015 Course Technical Faculty:

Martha A Delaney, University of Washington School of Medicine
Brian Johnson, University of Washington School of Medicine
Noraida Martinez-Rivera, University of Puerto Rico-Rio Piedras
Irma I. Torres-Vazquez, University of Puerto Rico-Rio Piedras

Course Founders and Supporters:

Andor, an Oxford Instrument Company
Bitplane, an Oxford Instrument Company
Chroma Technology Corporation
Electron Microscopy Sciences
GE Healthcare Life Sciences
The Histochemical Society
Vector Laboratories, Inc.
Carl Zeiss Microscopy LLC

Additional Course Supporters:

American Society of Investigative Pathology
Federation of American Societies of Experimental Biology (FASEB) Maximizing Access to Research Careers (MARC) Program
Imaging and Analysis, LLC
Journal of Histochemistry and Cytochemistry
Leica Biosystems
Micro-Tech Optical (NE), Inc.
SPOT Imaging Solutions, a division of Diagnostic Instruments, Inc.