September 22, 2014

Molecular Mycology: Current Approaches to Fungal Pathogenesis


Course Date: June 14 – June 30, 2015

Deadline: Feb. 9, 2015 | Online Application Form

2015 Flyer

2014 Lecture Schedule (for reference) (PDF)

Directors: Damian Krysan University of Rochester School of Medicine, and Xiaorong Lin, Texas A&M University

Course Mission:

Fungal diseases are significant causes of mortality and morbidity in both the developed and the developing world. The recent increases in the incidence and severity of invasive fungal infections are directly attributable to new susceptible patient populations. Examples of these large, at-risk populations include patients with AIDS; hospitalized patients being treated for cancer and autoimmune disorders; andthose receiving organ transplants. Despite this increasing threat, our understanding of the basic pathophysiology of fungal disease lags far behind our understanding of bacterial, parasitic and viral diseases. Furthermore, the number of antifungal therapies in clinical use is limited, and there is a paucity of novel antifungal strategies in the current drug pipeline. To address the need for more research in the area of fungal diseases, this course aims to:

a) increase students’ breadth of knowledge in fungal pathogenesis research

b) introduce and explore both standard and cutting edge model systems for the analysis of fungal virulence

c) create an environment that fosters interactions and idea-exchange among students, faculty, and the greater mycology research community.

Course material is suitable for advanced graduate students, post-doctoral fellows, research faculty, and clinician-scientists.

The specific objectives of the 2015 Molecular Mycology course:

  • To present the current conceptual models for the pathogenesis of medically important fungi, with a focus on the most frequently encountered pathogens – Candida, Cryptococcus, and Aspergillus
  • To train students in molecular manipulation of Candida, Cryptococcus, and Aspergillus
  • To provide hands on experience with mammalian, invertebrate, and cell culture models to assess virulence and analyze different types of host-pathogen interactions
  • To present a broad perspective on experimental issues pertinent to pathogenic fungi, such as the definition and determination of virulence, the determination of host responses relevant to infection, and the quantification of antifungal susceptibility
  • To instruct students in techniques relevant to the analysis of the function of fungal gene products such as determination of essentiality, microscopic analysis of morphology and fluorescent protein fusions, comparison of RNA expression profiles of wild-type and mutant strains, assessment of chromosome content
  • To provide insight into the clinical aspects of fungal diseases from the perspective of the host and the pathogen
  • To provide an introduction to tools for comparative genome and transcriptional analysis

This course is supported with funds provided by
Burroughs Wellcome Fund
Howard Hughes Medical Institute

2014 Course Directors:

Andrew Alspaugh, M.D. (Duke University School of Medicine)
Deborah Hogan, Ph.D. (Geisel School of Medicine at Dartmouth)

2014 Faculty-In-Residence:

Robert A. Cramer, Geisel School of Medicine at Dartmouth (Fungal Immunology, Aspergillus pathogenesis)
John E. Edwards, Harbor-UCLA Medical Center (Animal models of fungal diseases; clinical mycology)
Joseph Heitman, Duke University
(Research talk, career development, student interactions)
James B. Konopka, Stony Brook
 (Advanced Workshop in Microscopy)
Aaron Mitchell, Carnegie Mellon University (Genetics tools to study fungal pathogenesis)
Eleftherios Mylonakis, Brown University (Invertebrate models of fungal infections)
Judith Rhodes, University of Cincinnati School of Medicine (Clinical Microbiology)
Donald C. Sheppard, McGill University
 (Aspergillus fumigatus: Molecular Manipulation and Host-pathogen Interaction)
Robert Wheeler, University of Maine (Fungal mammalian cell interaction)
Theodore C. White, University Of Missouri – Kansas City (Antifungal Susceptibility and Resistance Mechanisms)


Judith Berman, Tel Aviv University / University of Minnesota
Maurizio Del Poeta, Stony Brook University
Amy Gladfelter, Dartmouth University
Damian Krysan, University of Rochester School of Medicine
Xiaorong Lin, Texas A and M University
Michael Lorenz, University of Texas – Houston Medical School
Joachim Morchausser, Universitat Wurzburg
Chad Rappleye, Ohio State University

Teaching Assistants:

Nora Grahl, Geisel School of Medicine at Dartmouth
Fabrice Gravelat, McGill University, Canada
Alex Hopke, University Of Maine
Connie Nichols, Duke University
Norma Solis, Harbor-UCLA Medical Center
Sven Wilger, Geisel School of Medicine at Dartmouth

Course coordinator:

Carol Edwards, Harbor-UCLA Medical Center