March 6, 2015

Zebrafish Development and Genetics


Course Date: August 9 – August 23, 2015

Deadline: April 10, 2015 | Online Application Form

2014 Schedule(pdf)

MBL Zebrafish Course Memorial Lecture

Course Website

Directors: Corinne Houart, Kings College, UK; Sharon Amacher, The Ohio State University; and Erez Raz, University of Muenster.

Over the past 20 years, the zebrafish has emerged as a powerful model system for the study of vertebrate development and disease. This intensive two-week course for advanced graduate students, postdoctoral fellows, and independent investigators will focus on the development and genetics of zebrafish. The course will cover time proven as well as novel technologies geared towards their application in zebrafish. Mornings and afternoons will be devoted mainly to laboratory exercises and the evenings to lectures and discussion. Limited to 22 students.

Lectures and labs in the first week will introduce students to early development of the zebrafish and to methods for manipulating and studying gene function, including genetic and small molecule screening, mapping and cloning; mRNA overexpression and functional knockdown approaches; cell fate mapping; mounting and imaging of antibody and in situ samples for publication ready documentation. In the second week, students will be introduced to labeling and visualizing individual cells, photoconversion and live cell imaging, cell transplantation/chimera analysis, and to performing behavioral tests on live animals. Informal ‘roundtable’ discussions held periodically during the course include the design and execution of mutant screens/reverse genetics, transgenesis and zebrafish breeding and husbandry.

Each laboratory exercise will be under the supervision of a senior faculty member who will be assisted by one or two junior faculty members. Senior faculty will give research lectures about their work, in addition to leading discussions on topics covered in the course.

This course is supported with funds provided by:
Howard Hughes Medical Institute
National Institute of Neurological Disorders and Stroke
National Institute on Deafness and Other Communication Disorders
National Institute of Mental Health
National Institute on Drug Abuse
National Institute of Child Health and Human Development
Aquatic Habitats

2014 Course Faculty and Lecturers:

Amacher, Sharon, The Ohio State University
Beattie, Christine, The Ohio State University
Collazo, Andres, California Institute of Technology
Ekker, Stephen, Mayo Clinic
Fadool, James, Florida State University
Karlstrom, Rolf, University of Massachusetts
Moens, Cecilia, Fred Hutchinson Cancer Research Center
Mullins, Mary, University of Pennsylvania School of Medicine
Poss, Kenneth, Duke University Medical Center
Raible, David, University of Washington
Raz, Erez, University of Muenster
Sagasti, Alvaro, University of California, Los Angeles
Schier, Alexander, Harvard University
Solnica-krezel, Lilianna, Washington University School of Medicine
Talbot, William, Stanford University
Woods, Ian, Ithaca College
Yelon, Deborah, University of California, San Diego