July 31, 2015

Zebrafish Development and Genetics

course_zebrafish

Course Date: August 9 – August 22, 2015

Deadline: April 10, 2015

Tentative 2015 Schedule (pdf)

2015 Memorial Lecture

Course Website

Directors: Corinne Houart, Kings College, UK; Sharon Amacher, The Ohio State University; and Erez Raz, University of Muenster.

Over the past 20 years, the zebrafish has emerged as a powerful model system for the study of vertebrate development and disease. This intensive two-week course for advanced graduate students, postdoctoral fellows, and independent investigators will focus on the development and genetics of zebrafish. The course will cover time proven as well as novel technologies geared towards their application in zebrafish. Mornings and afternoons will be devoted mainly to laboratory exercises and the evenings to lectures and discussion. Limited to 22 students.

Lectures and labs in the first week will introduce students to early development of the zebrafish and to methods for manipulating and studying gene function, including genetic and small molecule screening, mapping and cloning; mRNA overexpression and functional knockdown approaches; cell fate mapping; mounting and imaging of antibody and in situ samples for publication ready documentation. In the second week, students will be introduced to labeling and visualizing individual cells, photoconversion and live cell imaging, cell transplantation/chimera analysis, and to performing behavioral tests on live animals. Informal ‘roundtable’ discussions held periodically during the course include the design and execution of mutant screens/reverse genetics, transgenesis and zebrafish breeding and husbandry.

Each laboratory exercise will be under the supervision of a senior faculty member who will be assisted by one or two junior faculty members. Senior faculty will give research lectures about their work, in addition to leading discussions on topics covered in the course.

This course is supported with funds and equipment provided by:
Howard Hughes Medical Institute
National Institute of Neurological Disorders and Stroke
National Institute on Deafness and Other Communication Disorders
National Institute of Mental Health
National Institute on Drug Abuse
National Institute of Child Health and Human Development
Aquaneering
Pentair
Tecniplast

2015 Course Faculty and Lecturers:

Sharon Amacher (Ohio State University)
Christine Beattie (Ohio State University)
Andres Collazo (Caltech)
John Dowling (Harvard University)
Jim Fadool (Florida State University)
Michael Granato (University of Pennsylvania)
Corinne Houart (Kings College London)
Rolf Karlstrom (University of Massachusetts)
Cecilia Moens (Fred Hutchinson Cancer Research Center)
Kelly Monk (Washington University)
Mary Mullins (University of Pennsylvania)
Kenneth Poss (Duke University)
Dave Raible (University of Washington)
Erez Raz (Universitätsklinikum Münster)
Alex Schier (Harvard University)
Lila Solnica-Krezel (Washington University)
William Talbot (Stanford University)
Steve Wilson (University College London)
Ian Woods (Ithaca College)
Debbie Yelon (University of California San Diego)