Laboratory of Frederick Goetz -- Resources and Products

Leader of the Program in Scientific Aquaculture
Marine Resources Center ; Marine Biological Laboratory
The Marine Resources Center's recently established Program in Scientific Aquaculture conducts in-depth biological investigations of aquatic species cultured for biomedicine, commercial aquaculture, commercial fisheries, oceanography, conservation biology, and other industry.

Molecular Resources
Reagents are free of charge, except for any shipping and handling costs.

Yellow Perch
1) fully mature, postvitellogenic perch ovaries
2) fully mature, postvitellogenic perch ovaries stimulated in vivo with human chorionic gonadotropin
3) ovaries stimulated in vitro with 17 a lpha, 20beta dihydroxy-4-pregnen-3-one (0.1 m g/ml) for
    a) 6-7 hours
    b) 20-21 hours
    c) 24 hours
    d) 32 hours

Brook Trout
1) ovaries stimulated in vitro with phorbol ester/A23187 for
    a) 6 hours
    b) 12 hours
    c) 24 hours
2) ovaries at ovulation
3) ovaries post-ovulation
4) muscle
5) fat
6) tissue mix (kidney, liver, testis, brain, skin)

AF143003
Perca flavescens lysyl oxidase related protein homolog (PLO2) mRNA, complete cds

AF085251
Perca flavescens microtubule aggregate protein homolog mRNA, complete cds

AF223387
Salvelinus fontinalis ovarian cysteine protease inhibitor (OCPI) mRNA, complete cds

AF232215
Salvelinus fontinalis steroidogenic acute regulatory protein (StAR) mRNA, complete cds

AF077613
Salvelinus fontinalis S100-like calcium binding protein (S100) mRNA, complete cds

AF283287
Salvelinus fontinalis ferrodoxin-NADP(+) reductase mRNA, complete cds; nuclear gene for mitochondrial product

U03890
Salvelinus fontinalis antileukoproteinase precursor, mRNA, complete cds

AF158374
Salvelinus fontinalis prostaglandin endoperoxide synthase-1 (PGS-1) mRNA, complete cds

AF158373
Salvelinus fontinalis prostaglandin endoperoxide synthase-2 (PGS-2) mRNA, complete cds

AF077613
Salvelinus fontinalis S100-like calcium binding protein (S100) mRNA, complete cds

AF005026
Salvelinus fontinalis serine protease-like protein mRNA, complete cds

AF004739
Salvelinus fontinalis differentially regulated trout protein 1 mRNA, complete cds

U67854
Salvelinus fontinalis ovulatory protein-2 precursor, mRNA, complete cds

AF220435
Danio rerio steroidogenic acute regulatory protein (StAR) mRNA, complete cds

Bobe J, Goetz FW.
Cysteine protease inhibitor is specifically expressed in pre- and early-vitellogenic oocytes from the brook trout periovulatory ovary.
Mol Reprod Dev. 2001 Nov;60(3):312-8.

Bridgham JT, Bobe J, Goetz FW, Johnson AL .
Conservation of death receptor-6 in avian and piscine vertebrates.
Biochem Biophys Res Commun. 2001 Jun 29;284(5):1109-15.

Bobe J, Goetz FW .
Molecular cloning and expression of a TNF receptor and two TNF ligands in the fish ovary.
Comp Biochem Physiol B Biochem Mol Biol. 2001 Jun;129(2-3):475-81.

Roberts SB, Goetz FW
Differential skeletal muscle expression of myostatin across teleost species,
and the isolation of multiple myostatin isoforms
FEBS Lett. 2001 Vol. 491, No. 3, pp. 212-216

Patrick Bauer M, William Goetz F .
Isolation of gonadal mutations in adult zebrafish from a chemical mutagenesis screen.
Biol Reprod. 2001 Feb;64(2):548-54.
PMID: 11159357

Bobe J, Goetz FW.
A novel osteopontin-like protein is expressed in the trout ovary during ovulation.
FEBS Lett. 2001 Feb 2;489(2-3):119-124.
PMID: 11165234

Fullerton, AH; Lamberti, GA; Lodge, DM; Goetz, FW
Potential for Resource Competition between Eurasian Ruffe and Yellow Perch: Growth and RNA Responses in Laboratory Experiments
Transactions of the American Fisheries Society [Trans. Am. Fish. Soc.], vol. 129, no. 6, pp. 1331-1339, Nov 2000

Bobe J, Goetz FW.
A S100 homologue mRNA isolated by differential display PCR is down-regulated in the brook trout (Salvelinus fontinalis )
post-ovulatory ovary.
Gene. 2000 Oct 31;257(2):187-94.
PMID: 11080585; UI: 20534789

Planas JV, Athos J, Goetz FW, Swanson P.
Regulation of ovarian steroidogenesis in vitro by follicle-stimulating hormone and luteinizing hormone during sexual maturation in salmonid fish.
Biol Reprod. 2000 May;62(5):1262-9.
PMID: 10775175; UI: 20237180

Coffman MA, Pinter JH, Goetz FW.
Trout ovulatory proteins: site of synthesis, regulation, and possible biological function.
Biol Reprod. 2000 Apr;62(4):928-38.
PMID: 10727262; UI: 20191551

Roberts SB, Langenau DM, Goetz FW.
Cloning and characterization of prostaglandin endoperoxide synthase-1 and -2 from the brook trout ovary.
Mol Cell Endocrinol. 2000 Feb 25;160(1-2):89-97.
PMID: 10715542; UI: 20181763

Bobe J, Goetz FW.
A tumor necrosis factor decoy receptor homologue is up-regulated in the brook trout (Salvelinus fontinalis ) ovary at the completion of ovulation.
Biol Reprod. 2000 Feb;62(2):420-6.
PMID: 10642582; UI: 20111091

Langenau DM, Goetz FW, Roberts SB.
The upregulation of messenger ribonucleic acids during 17alpha, 20beta-dihydroxy-4-pregnen-3-one-induced ovulation in the perch ovary.
J Mol Endocrinol. 1999 Oct;23(2):137-52.
PMID: 10514552; UI: 99445407

Coffman MA, Goetz FW.
Trout ovulatory proteins are partially responsible for the anti-proteolytic activity found in trout coelomic fluid.
Biol Reprod. 1998 Sep;59(3):497-502.
PMID: 9716546; UI: 98384181

Duman JG, Li N, Verleye D, Goetz FW, Wu DW, Andorfer CA, Benjamin T, Parmelee DC.
Molecular characterization and sequencing of antifreeze proteins from larvae of the beetle Dendroides canadensis .
J Comp Physiol [B]. 1998 Apr;168(3):225-32.
PMID: 9591363; UI: 98253588

Hajnik CA, Goetz FW, Hsu SY, Sokal N.
Characterization of a ribonucleic acid transcript from the brook trout ( Salvelinus fontinalis ) ovary with structural similarities to mammalian adipsin/complement factor D and tissue kallikrein, and the effects of kallikrein-like serine proteases on follicle contraction.
Biol Reprod. 1998 Apr;58(4):887-97.
PMID: 9546717; UI: 98206794

Lee PH, Goetz FW.
Characterization of a novel cDNA obtained through differential-display PCR of phorbol ester-stimulated ovarian tissue from the brook trout ( Salvelinus fontinalis ).
Mol Reprod Dev. 1998 Feb;49(2):112-8.
PMID: 9444654; UI: 98107059

Garczynski MA, Goetz FW.
Molecular characterization of a ribonucleic acid transcript that is highly up-regulated at the time of ovulation in the brook trout ( Salvelinus fontinalis ) ovary.
Biol Reprod. 1997 Oct;57(4):856-64.
PMID: 9314590; UI: 97460165

Planas JV, Goetz FW, Swanson P.
Stimulation of brook trout ovarian steroidogenesis by gonadotropins I and II is mediated by the cyclic adenosine 3',5'-monophosphate/protein kinase A pathway.
Biol Reprod. 1997 Sep;57(3):647-54.
PMID: 9283003; UI: 97426299

Goetz FW.
Follicle and extrafollicular tissue interaction in 17alpha,20beta-dihydroxy-4-pregnen-3-one-stimulated ovulation and prostaglandin synthesis in the yellow perch ( Perca flavescens ) ovary.
Gen Comp Endocrinol. 1997 Jan;105(1):121-6.
PMID: 9000474; UI: 97153552

Bradley JA, Goetz FW.
The inhibitory effects of indomethacin, nordihydroguaiaretic acid, and pyrrolidinedithiocarbamate on ovulation and prostaglandin synthesis in yellow perch ( Perca flavescens ) follicle incubates.
Prostaglandins. 1994 Jul;48(1):11-20.
PMID: 7972876; UI: 95063112

Goetz FW, Bradley JA .
Stimulation of in vitro ovulation and contraction of brook trout ( Salvelinus fontinalis ) follicles by adrenaline through alpha-adrenoreceptors.
J Reprod Fertil. 1994 Mar;100(2):381-5.
PMID: 7912733; UI: 94293222

Hsu SY, Goetz FW.
Cellular mechanisms for orthovanadate-, phorbol ester-, and calcium ionophore-stimulated prostaglandin production in brook trout (Salvelinus fontinalis ) follicles.
Biol Reprod. 1993 Jun;48(6):1202-9.
PMID: 7686406; UI: 93305793

Goetz FW.
Involvement of protein kinase C in agonist-stimulated goldfish ovulation.
Biol Reprod. 1993 Apr;48(4):846-50.
PMID: 7683501; UI: 93250166

Sorensen PW, Goetz FW.
Pheromonal and reproductive function of F prostaglandins and their metabolites in teleost fish.
J Lipid Mediat. 1993 Mar-Apr;6(1-3):385-93. Review.
PMID: 8357996; UI: 93363873

Hsu SY, Goetz FW.
The effects of E and F prostaglandins on ovarian cAMP production and follicular contraction in the brook trout ( Salvelinus fontinalis ).
Gen Comp Endocrinol. 1992 Dec;88(3):434-43.
PMID: 1337050; UI: 93146360

Hsu SY, Goetz FW.
Oxoanions stimulate in vitro ovulation and signal transduction pathways in goldfish (Carassius auratus ) follicles.
Am J Physiol. 1992 Nov;263(5 Pt 1):E943-9.
PMID: 1332498; UI: 93072345

Hsu SY, Goetz FW.
Synergistic induction of ovulation and prostaglandin synthesis in goldfish (Carassius auratus ) follicles by sodium orthovanadate and hydrogen peroxide.
Biol Reprod. 1991 Dec;45(6):912-7.
PMID: 1805995; UI: 92215967

Goetz FW.
Compartmentalization of prostaglandin synthesis within the fish ovary.
Am J Physiol. 1991 May;260(5 Pt 2):R862-5.
PMID: 2035698; UI: 91241373

Ranjan M, Goetz FW.
Orthovanadate and fluoroaluminate stimulate inositol phosphate production and in vitro ovulation in goldfish ( Carassius auratus ) follicles.
Biol Reprod. 1990 Aug;43(2):323-34.
PMID: 2165827; UI: 90335378

Berndtson AK, Goetz FW.
Metallo-protease activity increases prior to ovulation in brook trout ( Salvelinus fontinalis ) and yellow perch ( Perca flavescens ) follicle walls.
Biol Reprod. 1990 Feb;42(2):391-8.
PMID: 2159810; UI: 90248511

Berndtson AK, Goetz FW, Duman P.
In vitro ovulation, prostaglandin synthesis, and proteolysis in isolated ovarian components of yellow perch ( Perca flavescens ): effects of 17 alpha,20 beta-dihydroxy-4-pregnen-3-one and phorbol ester.
Gen Comp Endocrinol. 1989 Sep;75(3):454-65.
PMID: 2792731; UI: 90006682

Sorensen PW, Hara TJ, Stacey NE, Goetz FW.
F prostaglandins function as potent olfactory stimulants that comprise the postovulatory female sex pheromone in goldfish.
Biol Reprod. 1988 Dec;39(5):1039-50.
PMID: 3219377; UI: 89118386

Berndtson AK, Goetz FW.
Protease activity in brook trout ( Salvelinus fontinalis ) follicle walls demonstrated by substrate-polyacrylamide gel electrophoresis.
Biol Reprod. 1988 Mar;38(2):511-6.
PMID: 3282556; UI: 88193226

DeManno DA, Goetz FW.
Steroid-induced final maturation in brook trout ( Salvelinus fontinalis ) oocytes in vitro: the effects of forskolin and phosphodiesterase inhibitors.
Biol Reprod. 1987 Jun;36(5):1321-32.
PMID: 3040136; UI: 87299926

DeManno DA, Goetz FW.
The effects of forskolin, cAMP, and cyanoketone on steroid-induced meiotic maturation of yellow perch ( Perca flavescens ) oocytes in vitro.
Gen Comp Endocrinol. 1987 May;66(2):233-43.
PMID: 3034715; UI: 87219797

Berndtson AK, Goetz FW.
Investigations on the control of in vitro spontaneous brook trout ( Salvelinus fontinalis ) ovulation.
Gen Comp Endocrinol. 1986 Jan;61(1):134-41.
PMID: 2416633; UI: 86083123

Goetz FW, Nagahama Y.
Effects of pH on in vitro ovulation of goldfish ( Carassius auratus ) oocytes.
J Exp Zool. 1985 Jul;235(1):81-5.
PMID: 3863880; UI: 86036017

Goetz FW, Cetta F.
Ovarian and plasma PGE and PGF levels in naturally ovulating brook trout ( Salvelinus fontinalis ) and the effects of indomethacin on prostaglandin levels.
Prostaglandins. 1983 Sep;26(3):387-95.
PMID: 6658000; UI: 84096523

Theofan G, Goetz FW.
The in vitro synthesis of final maturational steroids by ovaries of brook trout ( Salvelinus fontinalis ) and yellow perch ( Perca flavescens ).
Gen Comp Endocrinol. 1983 Jul;51(1):84-95.
PMID: 6884766; UI: 83288495

Cetta F, Goetz FW.
Ovarian and plasma prostaglandin E and F levels in brook trout ( Salvelinus fontinalis ) during pituitary-induced ovulation.
Biol Reprod. 1982 Dec;27(5):1216-21.
PMID: 7159663; UI: 83127690

Goetz FW, Smith DC, Krickl SP.
The effects of prostaglandins, phosphodiesterase inhibitors, and cyclic AMP on ovulation of brook trout ( Salvelinus fontinalis ) oocytes.
Gen Comp Endocrinol. 1982 Oct;48(2):154-60. No abstract available.
PMID: 6292040; UI: 83054575

Duffey RJ, Goetz FW.
The in vitro effects of 17 alpha-hydroxy-20 beta-dihydroprogesterone on germinal vesicle breakdown in brook trout (Salvelinus fontinalis ) oocytes.
Gen Comp Endocrinol. 1980 Aug;41(4):563-5. No abstract available.
PMID: 7409457; UI: 81005074

Goetz FW, Theofan G.
In vitro stimulation of germinal vesicle breakdown and ovulation of yellow perch ( Perca flavescens ) oocytes. Effects of 17 alpha-hydroxy-20 beta-dihydroprogesterone and prostaglandins.
Gen Comp Endocrinol. 1979 Mar;37(3):273-85. No abstract available.
PMID: 456868; UI: 79214406

Goetz FW, Bergman HL.
The effects of steroids on final maturation and ovulation of oocytes from brook trout ( Salvelinus fontinalis ) and yellow perch ( Perca flavescens ).
Biol Reprod. 1978 Mar;18(2):293-8. No abstract available.
PMID: 630027; UI: 78124350

Primer Aquisistion (DH)	Sequencing (DH)
Buffer Recipes (Roche)	Wizard Prep (DH)
Primer Dilution (DH)	Colony PCR (DH)
Westerns (SR)	Gel Electrophoresis (DH)
DNAzol (SR)	PCR (DH)
RNA Isolation (DH)	Glycerol Stock (DH)
Reverse Transcription (DH)	Cloning (DH)
Enzyme Digestion (DH)	Degenerative Primer Design (SR)
Chemi-Northerns (Pierce)