The Development of a Recirculating Culture System for Quahog (Mercenaria mercenaria)

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    With funding from the SouthEastern Massachusetts Aquaculture Cooperative ( SEMAC ) we are examining alternative methods for the culture of shellfish.   During the past year we have specifically been testing a small-scale, easy to operate, temperature controlled, recirculating aquaculture system for rearing quahog seed even at times of the year when growth is normally slow and mortality is high.  The primary system trial was run with slow growing quahogs provided by the town of Falmouth that were too small to be field set.   As we would not receive these animals until late October 2002 a preliminary system trial began in April with quahog seed stock (~2mm) from ARC.  This is will be referred to as Phase I, whereas the primary experiment of culturing of quahog juveniles (~6-18mm) provided by the town of Falmouth will be referred to as Phase II.   This website is designed to provide the public with a description of our research.  In addition,  we have provided information necessary for others to utilize this technology.


Background


        The primary objective of this project was to support and enhance sustainable growth within the aquaculture industry by developing an alternative system to be used in the nursery rearing of quahogs. The proposed system consists of a small-scale, easy to operate, temperature controlled, recirculating aquaculture system capable of cost effectively rearing quahog seed even at times of the year when growth is normally slow and mortality is high. The basic components and technology incorporated into this system have been used successfully to culture a variety of aquatic species, but to our knowledge they have never been applied to shellfish culture. By utilizing this technology to rear quahog seed, the hypothesis was that growers would potentially be able to accomplish two important goals: 
        1)    Because of the elevated temperatures in the recirculating system, seed will growth at a faster rate than seed reared in ambient water (especially during the early spring and late fall)
        2)    Growers could over-winter their sub-field plant size seed in a temperature-controlled environment, thus avoiding the typical 80-90% “winter-kill” mortality rate. 
       
        Overall, the technology developed by this project will contribute to the advancement of the aquaculture industry in southeastern Massachusetts by possibly allowing quahog seed producers and grow-out facilities to get a higher percentage of their product to a marketable size. Furthermore, the simplicity of the system in conjunction with the release of this informational could significantly increase the number of growers capable of maintaining and growing seed stock – this could substantially increase the overall quantity of quahog seed available for re-seeding waters of the Commonwealth.

Phase I

    As mentioned above, Phase I was the first use of the recirculating system, with the main objective of optimizing the system and working out in flaws in the system.  Below is a brief timeline, photos, and graph related to Phase I.
 

Week starting ...                                                        Status of the project

April 21

System is assembled at the Marine Resources Center.

April 28

Flow is modified to facilitate the autonomous removal of waste debris.  System is operating with seawater.

June 3

Upwellers constructed (see link below )

June 10

Quahog spat received, two upwelling nursery systems running.

June 24

Regular feeding and husbandry began

July 1

Algal Paste diet double to approx. 40 mls/day, two feedings a day

July 8

Debris in upweller system increased and was flushed with moderate water pressure

July 15

Adding approximately 14 gallons of water each week due to evaporation

July 22

Quahogs at Green Pond at 9mm compared to 3.5mm at MRC

July 29

Water temperature increased to ~25.  Flow reversed and decreased during feeding.

August 18

Growth rates significantly lower at MRC compared with Green Pond.
One upweller moved to 5um filtered flowing seawater with same feeding to elucidate contributing factors
Reverse flow during feeding halted, each upweller being fed 30 mls paste / twice a day.

September 23

Fish Farm drained and cleaned.  Both upwellers now in 55 gallon trash cans with flow through seawater.

 
 
Photos


Week of June 10

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b

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Week of August 18

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2

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4

5


Week of October 28





Water quality data was taken throughout Phase I and can be seen in the graph below.

 
 
 

chart



Phase I
I

    Last November, Paul Montegue (Falmouth Shellfish Officer) provided us with approximately 35,000 quahogs from two separate pump driven upwellers located at Green Pond and in West Falmouth.  The quahog juveniles ranged in size from 6-18 mm and were divided among 4 upwellers .  We cultured the juvenile quahogs in the upwellers for 20 weeks in room temperature seawater.  They were fed a diet of marine microalgae concentrate (Instant-Algae, Reed Mariculture).  Though there are reports that live algae can generate high grow rates, the culture of live algae is very labor intensive, requires considerable space, energy and expertise, and is usually the key factor preventing the successful culture of  shellfish.  Thus, we felt it important to investigate the use of prepackaged algal paste.

    Each day the quahogs were fed approximately 37 mls of microalgae concentrate which was equivalent to feeding 2% of the total wet tissue weight in dry algae.  The algae diet was a 1:1 mix of Tetraselmis and Isochrysis at a total cost of $336.00 (US) over the entire period. A small subsample of juvenile quahogs can be seen feeding in figure 4. Throughout the 20 weeks that quahog were held in the system, salinity, pH, ammonia, nitrite and nitrate all remained within acceptable levels.  During week 10, nitrite levels did begin to rise above baseline and  supplemental biofiltration was added by suspending a 0.4 cubic meter bag of Bio-Fill ™ media in the tank. Excess feces and detritus were siphoned every 5 weeks and dechlorinated fresh water was added periodically to replace evaporative loss.


Photos

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Conclusions
        Following acceptance of our Final Report to SEMAC we will provide additional information related to Phase II such as water quality data, results, and opinions on the feasibility of such a system.


Please check back to see additional information.


Please direct questions and comments related to this project to:


Bill Mebane
Marine Biological Laboratory
7 MBL St
Woods Hole, MA  02543
phone 508.289.7683
fax 508.289.7900


Links

Diagram and instructions for constructing upwelling nursery system  (PDF format)


SEMAC Website