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Laboratory of Rudolf Oldenbourg
Research Staff: Naoki Noda, Alex Valm, Grant B. Harris
Administrative Staff: Jane L. MacNeil
Contact: Tel: 508-289-7426 | E-mail: rudolfo@mbl.edu | Fax: 508-540-6902
Address: Lillie Building, Room 110
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Our research is inspired by advanced optical methods to study the architectural dynamics in living cells. We are developing a new type of polarized light microscope, the LC-PolScope, for the analysis of molecular order directly in living cells with unprecedented sensitivity, resolution and speed. Based on polarization measurements, we gain insight into submicroscopic structural parameters and non-invasively create contrast where structures are otherwise invisible. We seek interdisciplinary collaborations to conduct research in physical optics for the interpretation of image content and in computational methods for image enhancement and restoration. These physical and engineering projects are stimulated and guided by biological inquiries into the structural basis of cell function.
Senior Scientist Rudolf Oldenbourg
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Education:
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| 1981 |
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Ph.D. Physics, University of Konstanz, Konstanz, Germany |
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| 1976 |
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Diplomphysiker, Technical University, Munich, Germany |
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Selected Publications:
Guo, Y., Y. Liu, R. Oldenbourg, J. X. Tang and J. M. Valles, Jr. 2008. Effects of osmotic force and torque on microtubule bundling and pattern formation. Physical Review E 78: 041910.
Oldenbourg, R. 2008. Polarized light field microscopy: an analytical method using a microlens array to simultaneously capture both conoscopic and orthoscopic views of birefringent objects. J. Microsc. 231: 419-32.
Oldenbourg, R. 2007. Analysis of microtubule dynamics by polarized light. Methods Mol Med, 137, 111-123.
Janicke, M. A., L. Lasko, R. Oldenbourg and J. R. LaFountain, Jr. 2007. Chromosome malorientations after meiosis II arrest cause nondisjunction. Mol. Biol. Cell 18: 1645-56.
Barry, E., Z. Hensel, Z. Dogic, M. Shribak and R. Oldenbourg. 2006. Entropy-driven formation of a chiral liquid-crystalline phase of helical filaments. Phys. Rev. Lett. 96: Art. No. 018305.
LaFountain, J. R., Jr. and R. Oldenbourg. 2004. Maloriented bivalents have metaphase positions at the spindle equator with more kinetochore microtubules to one pole than to the other. Mol. Biol. Cell 15: 5346-5355.
Shribak, M. and R. Oldenbourg. 2004. Mapping polymer birefringence in three dimensions using a polarizing microscope with oblique illumination. In Biophotonics Micro- and Nano-Imaging, D. Anselmetti, Ed. Strasbourg, France, Proceedings of SPIE 5462: 57-67.
Oldenbourg, R. 2004. Polarization microscopy with the LC-PolScope. In Live Cell Imaging: A Laboratory Manual, D. L. Spector and R. D. Goldman, Eds. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press, pp. 205-237.
Shribak, M. and R. Oldenbourg. 2003. Techniques for fast and sensitive measurements of two-dimensional birefringence distributions. Appl. Opt. 42: 3009-3017.
Shribak, M. and R. Oldenbourg. 2002. Scanning aperture polarized light microscope: Observation of small calcite crystals using oblique illumination. In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX, J.-A. Conchello, C. J. Cogswell and T. Wilson, Eds. San Jose, Proceedings of SPIE 4621: 104-109.
Shribak, M., S. Inoue and R. Oldenbourg. 2002. Polarization aberrations caused by differential transmission and phase shift in high NA lenses: theory, measurement and rectification. Opt. Eng. 41: 943-954.
L. Liu, R. Oldenbourg, J. R. Trimarchi and D. L. Keefe. 2000. A reliable, noninvasive technique for spindle imaging and enucleation of mammalian oocytes. Nat. Biotechnol. 18: 223-225.
G. Danuser and R. Oldenbourg. 2000. Probing f-actin flow by tracking shape fluctuations of radial bundles in lamellipodia of motile cells. Biophys. J. 79: 191-201.
R. Oldenbourg. 1999. Polarized light microscopy of spindles. Methods in Cell Biology; Mitosis and Meiosis. C. L. Rieder, Ed. San Diego, Academic Press. 61: 175-208.
K. Katoh, K. Hammar, P. J. S. Smith and R. Oldenbourg. 1999. Birefringence imaging directly reveals architectural dynamics of filamentous actin in living growth cones. Mol. Biol. Cell 10: 197-210.
R. Oldenbourg, E. D. Salmon and P. T. Tran. 1998. Birefringence of single and bundled microtubules. Biophys. J. 74: 645-654.
R. Oldenbourg, R. 1996. A new view on polarization microscopy. Nature 381: 811-812.
R. Oldenbourg and G. Mei. 1995. New polarized light microscope with precision universal compensator. J. Microsc. 180: 140-147. |
