Laevis Stocks

For detailed information on specific lines or to request frogs, please email Xenopus@mbl.edu.

Now accepting orders for custom transgenic or mutant Xenopus laevis lines
NXR Custom Transgenics Information

Laevis Adult Stocks

Mem-GFP Transgenic

Mem-GFP Transgenic

NBT-GFP (Tubb2) Transgenic

NBT-GFP (Tubb2) Transgenic

 

 

 

 

 

Line Catalog  Number Image
 Ago2RFP
Reference: Brian Mitchell
NXR_0.0101
AlphaTubulin-UTR-RFP
Reference: John Wallingford
NXR_0.0074
Brachyury-Katushka
Breeding Type: Outbred
Description: 3856bp X. tropicalis Brachyury promoter driving expression of Katushka in muscles post-gastrulation.
Line Type: Transgenic
NXR_0.0050
BrainbowH
Breeding Type: Outbred
Description: RFP fluorescence; YFP or memCFP  fluorescence after Cre-mediated recombination. Germline transmission confirmed.
Line Type: Transgenic
Reference: Nature. 2007 Nov;450:56-62. 
NXR_0.0001
BrainbowL
Breeding Type: Outbred
Description: RFP fluorescence; YFP or memCFP  fluorescence after Cre-mediated recombination.
Line Type: Transgenic
Reference: Nature. 2007 Nov;450:56-62.
NXR_0.0002
BrainbowR
Breeding Type: Outbred
Description: RFP fluorescence; YFP or memCFP  fluorescence after Cre-mediated recombination.
Line Type: Transgenic
Reference: Nature. 2007 Nov;450:56-62.
NXR_0.0081
C5
Breeding Type: Outbred
Description: Whole embryo blue fluorescence; yellow fluorescence upon Cre or FLP recombination.
Line Type: Transgenic
Reference: Nucleic Acids Res. 2003 Apr;31:e44. Dev Dyn. 2006 Aug;235:2220-8. 
NXR_0.0003
CAG-GFP
Breeding Type: Outbred
Description: Fluorescence detectable in most tissues through development.
Line Type: Transgenic
Reference: Mead Lab
NXR_0.0004
CAG-Kikume GR NXR_0.0014
CAG-venus(YFP) LineB
Breeding Type: Outbred
Description: YFP fluorescence in most tissues.
Line Type: Transgenic
Reference: Nat Biotechnol. 2002 Jan;20:87-90. Dev Dyn. 2005 Jun;233:562-9. 
NXR_0.0006
CardiacActin-GFP
Breeding Type: Outbred
Description: Embryonic striated muscle including heart. m
Line Type: Transgenic
Reference: Dev Biol. 2002 May;5:57-70.  Development. 2003 Aug;130(16): 3865-76.
NXR_0.0007
Ccdc78-GFP
Reference: Brian Mitchell
NXR_0.0104
Cdh17inteGFP
Reference: Rachel Miller
NXR_0.0102
CentRFP
Reference: Brian Mitchel
NXR_0.0100
xCrys-mCherry:CMVmemGFP
Breeding Type: Outbred
Description: Ubiquitous membrane GFP expression.
Line Type: Transgenic
Reference: Custom transgenic made by the NXR.
 NXR_0.0012
CMV-Aequorin-GFP
Reference: Andrew Miller
NXR_0.0063
 CMV-Butterfly VSFP 1.2
Reference: Mike Levin
NXR_0.0092
CMV-GFP
Breeding Type: Outbred
Description: Whole embryo green fluorescence.
Line Type: Transgenic
NXR_0.0008
CMV-GFP-RFP
Breeding Type: Outbred
Description: Whole embryo green fluorescence; red fluorescence upon FLP recombination.
Line Type: Transgenic
Reference: Gene. 1996 Nov;180:225-7.   Nucleic Acids Res. 2001 Jun;29:E53-3.  Nucleic Acids Res. 2003 Apr;31:e44.
NXR_0.0009
CMV-H2BmRFP
Breeding Type: Outbred
Description: Unbiquitous nuclear RFP expression.
Line Type: Transgenic
Reference: Custom transgenic made by the NXR.
NXR_0.0094
CMV-VSFP2.42-SRL
Reference: Mike Levin
NXR_0.0095
Col2a1-GFP
Breeding Type: Outbred
Description: Chondrocytes.
Line Type: Transgenic
Reference: Int J Dev Biol. 2010;54(1):141-50.
NXR_0.0010
Deup1-GFP
Reference: Brian Mitchell
NXR_0.0103
Dria-GFP NXR_0.0059
EB3-EGFP
Reference: EXRC
NXR_0.0109
Ef1a-GFP
Breeding Type: Outbred
Description: Widespread expression expression apparent from mid-gastrula on.
Line Type: Transgenic
Reference: Gene. 1994 Sep;147:223-6.
NXR_0.0011
eGFP-ATP1a3
Description: GFP tagged-alpha subunit of neuronal form of sodium potassium-ATPase in rod photoreceptors
Reference: Sheila Baker
NXR_0.0122
GFP-Golgi
Reference: EXRC
NXR_0.0110
Elas-GFP
Breeding Type: Outbred
Description: Pancreas. F2 transgenic line.
Line Type: Transgenic
Reference: Mech Dev. 1999 Nov;88:221-7. Development. 2009 Jun;136:1791-800. Dev Biol. 2007 Apr;304(2): 786-99.   Curr Biol. 2003 Jan;13(2):105-15.
NXR_0.0013
ElasGFP: Tubb2-GCamp6S
Reference: Custom transgenic made by the NXR
NXR_0.0107
FLH-GFP
Breeding Type: Outbred
Description: Floating Head.
Line Type: Transgenic
Reference: 
EXRC
NXR_0.0017
FLK1-GFP
Breeding Type: Outbred
Description: Laevis 2.5kb promoter driving GFP expression in vascular endothelial cells.
Line Type: Transgenic
Reference:  Mead Lab. Dev Dyn. 2007 Oct;236:2808-17. 
NXR_0.0018
Foxi1-Katushka
Breeding Type: Outbred
Description:  2000bp X. tropicalis foxi I promoter driving expression of Katushka in epidermal cells (Ionocytes).
Line Type: Transgenic
NXR_0.0047
GATA1-GFP
Breeding Type: Outbred
Description: GATA-1 promoter and first intron driving expression of GFP in GATA-1 expressing cells (red blood cells and various other lineages, such as
Line Type: Transgenic
NXR_0.0019
HGEM-Noggin
Breeding Type: Outbred
Description: Lenses glow green; heat-shock inducible Noggin.
Line Type: Transgenic
Reference: Dev Cell. 2003 Sep;5:429-39. Mech Dev. 2006 Sep;123:674-88. 
NXR_0.0020
HGEM-Xtdkk1
Breeding Type: Outbred
Description: Heat-shock inducible Dkk1 to down-regulate Wnt/B-catenin signaling with a Gamma crystallin GFP selection marker.
Line Type: Transgenic
Reference: Dev Biol. 2008 Apr 15;316(2):323-35.
NXR_0.0021
Hsp:Cre1
Description: Cre recombinase drive by HSP70
Reference: EXRC
NXR_0.0121
HSP Cre1/C5
Reference: EXRC
NXR_0.0111
HyPer
Line Type: Transgenic
Reference: EXRC
NXR_0.0116
Insulin-memGFP
Breeding Type: Outbred
Description: Laevis 5kb insulin promoter driving memGFP expression; gamma crystallin mCherry selection
Line Type: Transgenic
Reference: Custom transgenic made by the NXR
NXR_0.0056
J-Strain
Breeding Type: J-Strain
Description: Inbred strain; homozygous MHC haplotype; genome sequenced.
Line Type: Wild Type
Reference: Virology. 2003 Jul; 311(2): 254-62.  Dev Growth Differ 1975; 17: 283.
NXR_0.0024
J-Strain Albino
Breeding Type: J-Strain
Description: TALEN induced tyrosinase mutant.
Line Type: Mutant
Reference: Custom strain made by the NXR
NXR_0.0041
K49
Reference: Brian Mitchell
NXR_0.0099
LMO2-Katushka ]
Line Type: Transgenic
Reference: EXRC
NXR_0.0114
LURP1-GFP
Breeding Type: Outbred
Description: Embryonic macrophages, definitive myeloid blood cells, restricted neural expression, epidermis after NF49.
Line Type: Transgenic
Reference: Mech Dev. 2002 Sep;117:173-86. Dev Growth Differ. 2006 Oct;48:499-512.
NXR_0.0025
MLC1V-GFP
Breeding Type: Outbred
Description: Heart myocardium.
Line Type: Transgenic
Reference:  Dev Dyn. 2005 Apr;232(4):1003-12. 
NXR_0.0026
MLC2-BicCGFP NXR_0.0090
MLC2-GFP
Breeding Type: Outbred
Description:  8kb myosin light chain promoter driving EGFP expression in the ventricular chamber type heart myocardium and interhyoid facial muscle.
Line Type: Transgenic
Reference: Development. 2004 Feb;131(3):669-79.
NXR_0.0027
mpeg-EGFP, crymCherry
Line Type: Transgenic
Reference: EXRC
NXR_0.0118
mPrx1
Line Type: Transgenic
Reference: EXRC
NXR_0.0106
Nkx2.5-GFP
Breeding Type: Outbred
Description: Early heart field, mesoderm, pharyngeal endoderm, spleen.
Line Type: Transgenic
Reference: Dev Biol. 2000 Nov 1;227(1):65-79.
NXR_0.0030
NXR-WT NXR_0.0031
Pax3-Katushka

Breeding Type: Outbred
Description:  2916bp X. laevis Pax3 promoter driving expression of Katushka in the neural ectoderm (neural crest).
Line Type: Transgenic
Reference:  Pax3 promoter from Roberto Mayor (Mauricio Moreno)

NXR_0.0049
pbin8LEF-dGFP NXR_0.0064
pHS-bCatenin-GFP:Elas-GFP NXR_0.0033
RFP-Mem
Line Type: Transgenic
Reference:  EXRC
NXR_0.0115
RFP-ER
Reference: EXRC
NXR_0.0113
Slc45a2 (Cr/Cas) NXR_0.0088
Slc45a2-GFP (KI) NXR_0.0089
SMAD3-GFP
Breeding Type: Outbred
Description: Dorsal mesoderm post-gastrulation, heart endocardium, widespread expression at larval stages.
Line Type: Transgenic
Reference: Mech Dev. 2011 May-Jun;128:303-15.
NXR_1.0035
Sox10-GFP
Reference: EXRC
NXR_0.0108
TUBB2-GFP NXR_0.0035
TUBB2-GFP-Aequorin
Reference: Andrew Miller
NXR_0.0062
TubulinA1-GFP NXR_0.0053
ubi-Gal4:UAS-venusTau:UAS-mCherryMoe
Description: GFP expression in microtubules and actin filaments, marker for integration selection: CFP expression in the eye.
Reference: EXRC
NXR_0.0123
Wild Caught NXR_0.0080
WT Albino NXR_0.0082
Xcry-GFP
Breeding Type: Outbred
Description: Lenses.
Line Type: Transgenic
Reference: Gene. 1993 Jun;128:189-95.
NXR_0.0036
Xic-GFP NXR_0.0051
XOPNTR
Breeding Type: Outbred
Description: Rod optsin promoter driving E. coli nitroreductase.
Line Type: Transgenic
Reference: FEBS Lett. 1998 Feb;423:117-21. Invest Ophthalmol Vis Sci. 2011 Jan;52:364-73.
NXR_0.0067
Y2
Breeding Type: Outbred
Description: Whole embryo blue fluorescence; yellow fluorescence upon Cre recombination.
Line Type: Transgenic
Reference: Nucleic Acids Res. 2003 Apr 15;31:e44.
NXR_0.0040