Transgenic Laevis

Transgenic Lines Image
Xla.Tg(CMV:Brainbow-1.0H)NXR

NXR_0.0001

Description: (Brainbow H) Default state is ubiquitous whole cell red fluorescence. Following Cre induced recombination cells will have membrane yellow or membrane cyan fluorescence.

Reference: Nature. 2007 Nov;450:56-62. 

Xla.Tg(CMV:Brainbow-1.0L)NXR

NXR_0.0002

Description: (BrainbowL) Default state is ubiquitous whole cell red fluorescence. Following Cre induced recombination cells will have whole cell yellow or whole cell cyan fluorescence.

Reference: Nature. 2007 Nov;450:56-62.

Source: Helen Willsey

Source: Helen Willsey

Xla.Tg(LCMV:eYFP,eCFP)Ryff

NXR_0.0003

Description: (C5) Whole embryo blue fluorescence; yellow fluorescence upon Cre or FLP recombination.

Available as frozen sperm, please allow 8 months for live animals.

Reference: Nucleic Acids Res. 2003 Apr;31:e44. Dev Dyn. 2006 Aug;235:2220-8. 

Source: EXRC

Source: EXRC

Xla.Tg(CAG:GFP)Mead

NXR_0.0004

Description: Fluorescence detectable in most tissues through development.

Available as frozen sperm, please allow 8 months for live animals.

Reference: Mead Lab

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Xla.Tg(CAG:Venus)Ueno

NXR_0.0006

Description: (CAG-venus(YFP) LineB) pCAGGS driving Venus YFP expression under the control of the CAG promoter and is followed by the rabbit β-globin polyA signal sequence.

Reference: Nat Biotechnol. 2002 Jan;20:87-90. Dev Dyn. 2005 Jun;233:562-9. 

Source: EXRC

Source: EXRC

Source: EXRC

Source: EXRC

Xla.Tg(actc1:GFP)Mohun

NXR_0.0007

Description: (Cardiac actin:GFP) 580bp of X. laevis actc1b gene driving GFP expression. Embryonic striated muscle including heart.

Reference: Dev Biol. 2002 May;5:57-70.  Development. 2003 Aug;130(16): 3865-76.

cardiacactingfp

Source: NXR

Xla.Tg(CMV:GFP)Murray

NXR_0.0008

Description: (CMV-GFP) CMV promoter driving GFP expression, Whole embryo green fluorescence.

Reference:

Source: EXRC

Source: EXRC

EXRC

Source: EXRC

Xla.Tg(CMV:GFP,RFP)Amaya

NXR_0.0009

Description: (CMV-GFP-RFP) CMV IE94 promoter driving GFP flanked by FRT sites, after FLP  recombination CMV drives RFP. Whole embryo green fluorescence, red fluorescence upon FLP recombination

Available as frozen sperm, please allow 8 months for live animals.

Reference: Gene. 1996 Nov;180:225-7.   Nucleic Acids Res. 2001 Jun;29:E53-3.  Nucleic Acids Res. 2003 Apr;31:e44.

Source: EXRC

Source: EXRC

Source: EXRC

Source: EXRC

Source: EXRC

Source: EXRC

Xla.Tg(Xtr.col2a1:GFP;cryga:GFP)Hank

NXR_0.0010

Description: (Col2a1-GFP) X. tropicalis collagen 2α1 promoter and first intron driving eGFP, γ-cry driving GFP marker. GFP expression in cartilaginous skeleton.

Reference: Int J Dev Biol. 2010;54(1):141-50. Xenbase.

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Xla.Tg(eef1a1:GFP;cryga:GFP)Krieg

NXR_0.0011

Description: (Ef1α-GFP) Eef1a1 gene driving GFP expression, γ-cry driving GFP marker. Widespread  GFP expression apparent from mid-gastrula on.

Available as frozen sperm, please allow 8 months for live animals.

Reference: Gene. 1994 Sep;147:223-6.

Source: NXR

Source: NXR

Xla.Tg(CMV:memGFP,cryga:mCherry)NXR 

NXR_0.0012

Description: (CMV:memGFP) CMV promoter driving GFP in cell membrane, γ-cry driving mCherry marker. Ubiquitous membrane GFP expression and mCherry in lens.

Reference: Custom transgenic generated by the NXR.

Source: NXR

Source: NXR

Xla.Tg.(Rno.elas:GFP)Jmws

NXR_0.0013

Description: (Elas-GFP) Rat elastase promoter driving GFP. GFP expression in the pancreas.

Reference: Mech Dev. 1999 Nov;88:221-7. Development. 2009 Jun;136:1791-800. Dev Biol. 2007 Apr;304(2): 786-99.   Curr Biol. 2003 Jan;13(2):105-15. Xenbase.

Source: NXR

Source: NXR

Xla.Tg(CAG:KikGR)Flc

NXR_0.0014

Description: (CAG-KikumeGR) CAG promoter driving green-to-red photoconvertible fluorescent protein, Kikume Green-Red (KikGR)

Reference: BMC Development Biology 2009 Sept; 9:49

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Xla.Tg(cryga:RFP,CMV:HyPer-YFP) Amaya

Description: (HyPer) CMV promoter driving YFP of H2O2 indicator, HyPer. γ-cry driving RFP. Ubiquitous HyPer YFP expression, marker for integration selection; RFP expression in lens.

Reference: EXRC.

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Xla.Tg(Dre.noto:GFP)1Amaya

NXR_0.0017

Description: (FLH-GFP) Zebrafish flh promoter driving GFP in the pineal gland and posterior notochord.

Reference:  EXRCConcha, M., et. al. (2003). Local tissue interactions across the dorsal midline of the forebrain establish CNS laterality. Neuron; 39(3):423-38. Xenbase.

Source: EXRC

Source: EXRC

Xla.Tg(kdr:GFP)Mead

NXR_0.0018

Description: (FLK1-GFp) 2.5kb X. laevis flk-1 promoter driving GFP. GFP expression in vascular endothelial cells.

Reference: Mead Lab. Dev Dyn. 2007 Oct;236:2808-17.  Xenbase.

Source: NXR

Source: NXR

Source: NXR

Source: NXR

flk1gfp_1

Source: NXR

Xla.Tg(gata1:GFP)Mead

NXR_0.0019

Description: (GATA1-GFP) GATA-1 promoter and first intron driving expression of GFP, sleeping beauty. GFP expression in red blood cells.

Reference: Mead Lab. Xenbase.

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Xla.Tg(cryga:GFP;hsp70-nog)Jmws

NXR_0.0020

Description: (HGEM-Noggin) Lenses glow green; heat-shock inducible Noggin.

Reference: Dev Cell. 2003 Sep;5:429-39. Mech Dev. 2006 Sep;123:674-88.  Xenbase.

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Xla.Tg(hsp70:Xtr.dkk1;crya:GFP)Jmws

NXR_0.0021

Description: (HGEM-Xtdkk1) Heat-shock inducible Dkk1 to down-regulate Wnt/B-catenin signaling with a Gamma crystallin GFP selection marker.

Reference: Dev Biol. 2008 Apr 15;316(2):323-35. Xenbase.

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Xla.Tg(slurp1l:GFP)Mohun

NXR_0.0025

Description: (LURP1-GFP) 5kb of X.laevis slurp1l gene driving eGFP. Embryonic macrophages, definitive myeloid blood cells, restricted neural expression, epidermis.

Reference: Mech Dev. 2002 Sep;117:173-86. Dev Growth Differ. 2006 Oct;48:499-512. Xenbase.

lurp1gfp_1

Source: NXR

Source: NXR

Source: NXR

Xla.Tg(mlc3:eGFP)Mohun

NXR_0.0026

Description: (MLC1V_GFP) 8kb of X. laevis mlc3 gene driving enhanced GFP. GFP expression in heart mycoardium.

Reference:  Dev Dyn. 2005 Apr;232(4):1003-12.  Xenbase.

Source: EXRC

Source: EXRC

Source: EXRC

Source: EXRC

Xla.Tg(myl2:eGFP)Mohun

NXR_0.0027

Description: (MLC2-GFP) 3kb of X. laevis myosin light chain promoter driving eGFP. eGFP expression in ventricular charmber, heart myocardium, and interhyoid facial muscle.

Reference: Development. 2004 Feb;131(3):669-79. Xenbase.

Xla.Tg.(nkx2-5:GFP)Mohun

NXR_0.0030

Description: (Nkx2.5-GFP) 8kb promoter of X. laevis nkx2-5 gene driving GFP expression. Early heart field, mesoderm, pharyngeal, endoderm, spleen.

Reference: Dev Biol. 2000 Nov 1;227(1):65-79. Xenbase.

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Xla.Tg(hsp70:CActnnb1-GFP;Rno.elas:GFP)Jmws

NXR_0.0033

Description: (pHS-bCatenin-GFP:Elas-GFP) Generated via REMI (without restriction enzyme) with a cassette containing heatshock promoter driving expression of N-term truncated beta-catenin (stabilized CA form) tagged with GFP. Presumably the cassette also included elas:GFP but that is not described in the paper. The original vector from which pHsβcat∗GFP was derived was named pCH85.

Reference: Developmental Biology, 2008, Sept 15; 316(2): 323-335.

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Xla.Tg(smad3:eGFP;cryga:GFP)Mohun

NXR_0.0034

Description: (SMAD-GFP) 1.5kb of X. laevis Smad3 gene driving eGFP. Dorsal mesoderm post gastrulation, heart endocardium, widespread expression at larval stages.

Reference: Mech Dev. 2011 May-Jun;128:303-15. Xenbase.

smad3

Source: NXR

Xla.Tg(tubb2b:mapt-GFP)Amaya

NXR_0.0035

Description: (NBT/Tubb2-GFP) 3481bp  promoter and 5’UTR of tubb2b driving expression pf mapt-fusion. Green fluorescence in neaural tissue.

Reference: Xenbase.

Source: NXR

Source: NXR

tubb2gfp

Source: NXR

Xla.Tg(cryga:eGFP)Amaya

NXR_0.0036

Description: (xCry-GFP) The crystalline –GFP construct was generated by sub-cloning 2.2 kb a fragment of the crystalline genomic DNA encompassing the promoter region (received from Robert Grainger), upstream of an enhanced version of the GFP3.

Reference: Gene. 1993 Jun;128:189-95.

Source: EXRC

Source: EXRC

Xla.Tg(CMV:eYFP,eCFP)1Ryff

NXR_0.0040

Description: (Y2) Whole embryo blue fluorescence; yellow fluorescence upon Cre recombination.

Reference: Nucleic Acids Res. 2003 Apr 15;31:e44.

y2cmvgfp

Source: NXR

Xla.Tg(Xtr.foxi1:katushka;cryga:Venus)EXRC

NXR_0.0047

Description: (Foxi1-Katushka) 2000bp X. tropicalis foxi I promoter driving expression of Katushka, γ-cry driving Venus marker. Katushka expression in epidermal cells and Venus in lens as integration marker.

Reference: EXRC. Xenbase.

Source: EXRC

Source: EXRC

Xla.Tg(pax3:Katushka;cryga:Venus)EXRC

NXR_0.0049

Description: (Pax3-Katushka) 2916bp X. laevis Pax3 promoter driving expression of Katushka in the neural ectoderm (neural crest).

Reference:  Pax3 promoter from Roberto Mayor (Mauricio Moreno). Xenbase.

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Xla.Tg(Xtr.tbxt:Katushka;cryga:Venus)EXRC

NXR_0.0050

Description: (Brachyury-Katushka) 3856bp X. tropicalis Brachyury promoter driving expression of Katushka, γ-cry driving Venus marker. Katushka expression in muscles post-gastrulation and Venus in lens as integration marker.

Reference: EXRC

Source: EXRC

Source: EXRC

Source: EXRC

Source: EXRC

Xla.Tg(Xtr.cdknx:GFP)Papal

NXR_0.0051

Description: (Xic-GFP) 4.7 kb X. tropicalis  p27 Xic1 promoter driving GFP expression in the neural tube and the eye.

Available as frozen sperm, please allow 8 months for live animals.

Reference: EXRC. Carruthersa, S., et. al. (2003) “Depletion of the cell-cycle inhibitor p27Xic1 impairs neuronal differentiation and increases the number of ElrCþ progenitor cells in Xenopus tropicalis.” Mech of Dev. 120; 607–616. Xenbase.

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Xla.Tg(Rno.tuba1a:GFP)Brown

NXR_0.0053

Description: (TubulinA1-GFP) Rat tubulin a-1 promoter fused in grame to GFP. GFP expression in the nervous system and later in growing limbs.

Reference: EXRC. Marsh-Armstrong, N., et. al. (1999) “Germ-line transmission of transgenes in Xenopus laevis.” PNAS 96, 14389-14393. Xenbase.

EXRC

Source: EXRC

Xla.Tg(ins:memGFP,cryga:mCherry)

NXR_0.0056

Description: (Insulin-memGFP) Laevis 5kb insulin promoter driving memGFP expression; gamma crystallin mCherry selection

Reference: Transgenic generated by Donald Brown.

gfp-bf-head

Source: NXR

Source: NXR

Source: NXR

Xla.Tg(CMV:eGFP-OMP25)Wtnbe

NXR_0.0059

Description: (Dria-GFP) CMV driving eGFP tagged OMP25 transmembrane segment. GFP expression of mitochondria membranes.

Reference: Dev Growth Differ. October 1, 2012; 54 (8): 767-76.

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Xla.Tg(tubb2b:GFP,Aequorin)NXR

NXR_0.0062

Description: (Tubb2-GFP-Aequorin)

Reference: Custom transgenic generated by the NXR for Andrew Miller.

Xla.Tg(CMV:Aequorin,GFP)NXR

NXR_0.0063

Description: (CMV-Aequorin-GFP) Aequorin is a Ca2+ dependent bioluminescence photoprotein from hydrozoan A. Displays an emission peak at 470nm (blue light).

Reference:Custom transgenic generated by the NXR for Andrew Miller.

Xla.Tg(WntREs:dEGFP)Vlemx

NXR_0.0064

Description: (pbin7LEF-GFP/Wnt Reporter) Construct contains a synthetic Wnt-responsive promoter – consisting of 7 copies of a TCF/LEF1 binding DNA element, a minimal TATA box and a reporter gene encoding destabilized EGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence. GFP expression of dynamic patterns of Wnt/β-catenin signalling activity.

Reference: Proc Natl Acad Sci USA. 2010 Sep 14; 107(37): 16160-16165.

pbin8lefgfp

Source: NXR

Xla.Tg(rho:Eco.nfsA)Zuber

NXR_0.0067

Description: (XOPNTR) Rod optsin promoter driving E. coli nitroreductase.

Reference: FEBS Lett. 1998 Feb;423:117-21. Invest Ophthalmol Vis Sci. 2011 Jan;52:364-73.

Xla.Tg(Mmu.tal1:GFP)Patnt

NXR_0.0069

Description: (SCL-GFP) Mouse SCL +19 enhancer which contains a GATA-ETS-GATA core motif driving GFP. Expression in haematopoietic precursors.

Reference: EMBO J. 2002 Jun 17;21(12):3039-50. Mol Cell Biol. 2010 Dec;30(24):5741-51. Xenbase. EXRC.

Source: NXR

Source: NXR

Xla.Tg(tuba1a:MyrPalm-mRFP1)NXR

NXR_0.0074

Description: (AlphaTubulin-UTR-RFP) tubulin alpha 1a promoter driving expression of membrane bound red fluorescent protein primarily in the ciliated epidermal cells.

Reference: Custom transgenic generated by the NXR for John Wallingford.

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Xla.Tg(CMV:Brainbow-2.1R)NXR

NXR_0.0081

Description: (BrainbowR) Default state is nuclear green fluorescence. Following Cre induced recombination cells will have whole cell red, membrane cyan, nuclear green, or whole cell yellow fluorescence.

Reference: Nature. 2007 Nov;450:56-62.

Xla.Tg(CMV:VSFP_Butterfly1.2)NXR

NXR_0.0092

Description: (CMV-Butterfly VSFP1.2) VSFP is voltage sensitive fluorescence protein. VSFP-Butterfly 1.2 enables optical recordings at high spatial resolution with intrinsic assignment of recorded signals to VSFP-targeted (neural) cell populations.

Reference: Custom transgenic generated by the NXR for Mike Levin.

Xla.Tg(CMV:hist2h2be-RFP;cryga:Venus)NXR

NXR_0.0094

Description: (CMV-H2BmRFP) CMV promoter driving RFP in nuclear membrane, γ-cry driving Venus marker. Ubiquitous nuclear RFP expression, and Venus expression in lens.

Reference: Custom transgenic generated by the NXR.

cmvh2brfp

Source: NXR

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Source: MBL Embryology 2017

Xla.Tg(CMV-VSFP2.42_SRL)NXR

NXR_0.0095

Description: (CMV-VSFP2.42-SRL)

Reference: Custom transgenic generated by the NXR for Mike Levin.

Xla.Tg(CMV:cetn4-RFP)NXR

NXR_0.0100

Description: (Centrin-RFP) CMV promoter driving RFP and (cetn4) fused with RFP. RFP expression in the microtubules of cilia/ciliated cells.

Reference: Custom transgenic generated by the NXR for Brian Mitchell.

Xla.Tg(Dre.cdh17:eGFP)NXR

NXR_0.0102

Description: (Cdh17inteGFP) Zebrafish(Dre) Li-cadherin (CDH17) driving eGFP. GFP expression in the pronephros/pronephric tubes.

Reference: Custom transgenic generated by the NXR for Rachel Miller. Genes 2018, 9(4).

cdh17integfp

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Xla.Tg(tuba1a:deup1-eGFP)NXR

NXR_0.0103

Description: (Deup1-GFP) Tubulin promoter driving Deup1 fused to eGFP. eGFP expression of deuterosomes and centrioles specifically in multi-ciliated cells. Marker for visualizing centrioles.

Reference: Custom transgenic generated by the NXR for Brian Mitchell. Xenbase.

deup1-gfp_2

Source: NXR

Source: NXR

Source: NXR

Xla.Tg(Mmu.prrx1:GFP3)Tamura

NXR_0.0106

Description: (mPrx1-GFP) GFP labels mesenchymal cells in limb bud and limb blastema.

Reference: EXRCDev Biol 304 (2007) 675–686. Xenbase.

mprx1

Source: NXR

Xla.Tg(tubb2b:GCaMP6s,Rno.elas:GFP)NXR

NXR_0.0107

Description: (ElasGFP:Tubb2-GCaMP6s) Neaural expression of the green Ca2+ sensor, GCaMP6s, and expression of GFP in the pancreas

Reference: Custom transgenic generated by the NXR.

Source: Hollis Cline

Source: Hollis Cline

Source: Hollis Cline

Source: Hollis Cline

Xla.Tg(sox10:GFP)Jpsj

NXR_0.0108

Description: (Sox10-GFP) Sox10:GFP-HS4-Isce1 transgene 4.7kb upstream from sox10 transcription factor. GFP expression in the migratory cranial neural crest cells.

Reference: EXRC. Xenbase.

Sergei Sokol

Source: Sergei Sokol

Source: Sergei Sokol

Source: Sergei Sokol

Sergei Sokol

Source: Sergei Sokol

Xla.Tg(CMV:Hsa.EB3-eGFP)Ueno

NXR_0.0109

Description: (EB3-GFP) CMV driving eGFP of human EB3. Ubiquitous expression of eGFP in microtubule plus ends

Reference: EXRCTakagi et al. 2013 Dev. Growth Differ 55, 422-433.

 

eb3gfp

Source: NXR

Xla.Tg(CMV:Hsa.B4GALT1-eGFP)Ueno

NXR_0.0110

Description: (GFP-Golgi) CMV driving GFP of hb4GalT transgene. Ubiquitous expression of eGFP in the golgi appartus.

Reference: EXRCTakagi et al. 2013 Dev. Growth Differ 55, 422-433.

Source: EXRC

Source: EXRC

Source: NXR

Source: NXR

Source: EXRC

Source: EXRC

Xla.Tg(hsp70:eYFP)Ryff

NXR_0.0111

Description: (HSP Cre1(C5)) Cross of HSP:Cre1 and C5, heat shocked in embryogensis and EYFP positive tadpoles were grown.

Reference: EXRC.

Xla.Tg(CMV:calr-RFP)Ueno

NXR_0.0113

Description: (RFP-ER) CMV promoter driving RFP in CALR targeting sequence. Ubiquitous expression of RFP in the endoplasmic reticulum.

Reference: EXRCTakagi et al. 2013 Dev. Growth Differ 55, 422-433. Xenbase.

Source: EXRC

Source: EXRC

Source: NXR

Source: NXR

Source: EXRC

Source: EXRC

Xla.Tg(Xtr.lmo2:Katushka;cryga:Venus)EXRC

NXR_0.0114

Description: (LMO2-Katushka) 4775bp X. tropicalis LMO2 promtoer driving Katushka, γ-cry driving VenusGFP marker. Blood marked with red fluroescent protein Katushka, and Venus YFP variant expressed in the lens.

Reference: EXRC. Xenbase.

whole-bro-red-8s-exposure

Source: NXR

Source: NXR

Source: NXR

Xla.Tg(CMV:mRFP)Ueno

NXR_0.0115

Description: (RFP-Mem) CMV promoter driving RFP in cell membrane. Ubiquitous membrane RFP expression.

Reference:  EXRCTakagi et al. 2013 Dev. Growth Differ 55, 422-433.

rfpmem

Source: NXR

Xla.Tg(Dre.mpeg1:eGFP;cryga:mCherry)Amaya

NXR_0.0118

Description: (mpeg-EGFP,crygamCherry) Zebrafish mpeg promoter driving GFP, γ-cry driving mCherry marker. GFP expression in the macrophages and mCherry in the lens.

Reference: EXRCEllett, F. et. al. (2011) “Mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish”. Blood; 117(4):e49-56.

mpegegfpcrymcherry

Source: NXR

Xla.Tg(hsp70:Cre)Ryff

NXR_0.0121

Description: (Hsp:Cre1) Cre recombinase drive by HSP70

Reference: EXRCRoose,M., et. al. (2009) “Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis.” Trans Res. 18, 595-605.

Xla.Tg(rho:eGFP-atp1a3)Baker

NXR_0.0122

Description: (eGFP-ATP1a3) Xenopus rhodopsin promoter driving expression of eGFP tagged neuronal α3 isoform of the Na+/K+-ATPase in the rod photoreceptors.

Referenece: Traffic. 2015 Dec;16(12):1239-53. Xenbase.

Source: NXR

Source: NXR

Xla.Tg(Hsa.UBC:Gal4-VP16;cryga:CFP;UAS:mCherry-msn;UAS:Venus-mapt)Amaya

NXR_0.0123

Description: (Ubi-Gal4:UAS-venusTau:UAS-mCherryMoe)

Ubiquitous red fluorescence marking F-actin and green fluorescence marking microtubules. Cyan fluorescence marking the eye lens. Line was generated by digestion of three plasmids with Asp718 and then co-injection via REMI. First plasmid: 1.5 kb of human ubiquitin C promoter driving expression of Gal4-VP16 and gamma crystalin promoter driving expression of CFP. Second plasmid: UAS driving expression of mCherry tagged moesin. Third plasmid: UAS driving expression of Venus tagged microtubule associated protein tau.

Reference: EXRC

ubiqgal4uasvenustauuasmcherrymoe

Source: NXR

Xla.Tg(tubb2b:ChIEF,Rno.elas:GFP)NXR

NXR_0.0125

Description: (Elas:GFP,Tubb2bChIEF) Neaural expression of blue light photoinducible channelrhodopsin, ChIEF, and expression of GFP in the pancreas.

Reference: Custom transgenic generated by the NXR.

chief

Source: NXR

Xla.Tg(hsp70:DNyap1-GFP,cryga:tdTomato)Tamura

NXR_0.0126

Description: (HSP70:dnYAP-2A-GFP,gCry:tdTomato) 330 bp of promoter of the X. laevis hsp70 gene driving the expression dominant negative form of Yap (dnYap) tagged with GFP via 2A peptrides of 2.2 kb gamma crystalline promoter driving tdTomato.

Reference: EXRC.

Source: EXRC

Source: EXRC

Xla.Tg(Hsa.UBC-Gal4;UAS:HyPer-YFP)Amaya

NXR_0.0127

Description: Ubiquitous expression of ROS indicator, Hyper.

Reference: EXRC.

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Xla.Tg.(hsp70:DNfgfr-Venus:cryga:RFP)Amaya

NXR_0.0128

Description: (HSP-XFDvenus; cryRFP) HSP70 promoter driving dominant negative FGFR with crystallin-RFP.

Reference: EXRC.

Xla.Tg(Hsa.UBC:COX8A-dsRed;cryga:GFP)EXRC

NXR_0.0129

Description: (Ubic dsRed-Mito) 1.5kb of UbiC promoter driving dsRed and COX8, γ-cry driving Venus marker. Ubquitous dsRed expression in mitochondria, and Venus in lens.

Reference: EXRC.

Xla.Tg(CMV:H3K9ac-eGFP)Ueno

NXR_0.0130

Description: (Mintbody/CMV-H3K9ac-EGFP) CMV promoter driving eGFP of h3k9ac transgene. Ubiquitous GFP histone expression.

Reference: Ueno.  Genes Cells. 2016 21:358–369. Sci Rep. 2013; 3:2436.

Source: Ueno Lab

Source: Ueno Lab

Source: Ueno Lab

Source: Ueno Lab

Source: Ueno Lab

Source: Ueno Lab

Source: Ueno Lab

Source: Ueno Lab

Xla.Tg(pax8:GFP)Ogino

NXR_0.0132

Description: (Pax8-GFP) Pax8 driving GFP. GFP expression of kidney and head.

Reference: Nat Commun. 2012 May 22;3:848. Xenbase.

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Xla.Tg(CAG:SCAT3)Ueno

NXR_0.0134

Description: (NIBB6/Scat3) CAG-seCFP-Venus (an enhanced yellow fluorescent protein with efficient maturation property), containing the fluorescence indicator molecule SCAT3 and the rabbit β-globin polyA signal sequence, under control of the CAG promoter. SCAT3 is a hybrid protein of seCFP-Venus linked with the caspase-3 recognition sequence (DEVD) in pCAGGS.

Reference: Ueno LabKominami, K. et al., 2006 Genes Cells 11:701-711Takagi C. et al. 2013 Dev. Growth Differ 55, 422-433.

Source: NXR

Source: NXR

Xla.Tg(CMV:hist2h2be-GFP;CMV:mRFP)Flc

NXR_0.0139

Description: (CMV:H2B-GFP;CMV:mRFP) Cross of CMV:mRFP parent created CMV IE94 promoter in pCS2+ backbone, RFP inserted at BstBI and linearized with NotI; and CMV:H2B-GFP parent. Ubiquitous GFP expression of nuclear membrane and ubiquitous RFP expression of cell membrane.

Reference: Conlon Lab. H2B Xenbase.

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Source: NXR