Laevis Stocks

For detailed information on specific lines or to request frogs, please email Xenopus@mbl.edu.

Now accepting orders for custom transgenic or TALEN Xenopus laevis lines
NXR Custom Transgenics Information

Laevis Adult Stocks

Mem-GFP Transgenic

Mem-GFP Transgenic

NBT-GFP (Tubb2) Transgenic

NBT-GFP (Tubb2) Transgenic

 

 

 

 

 

Strain Date of Birth Catalog  Number Type
 

Reference: Brian Mitchell

7/15 NXR_0.0101 Transgenic
 

Reference: Wade Powell

4/14 NXR_0.0071  Mutant 

Reference: Wade Powell


8/14 NXR_0.0075 Mutant

Reference: Wade Powell

4/14 NXR_0.0070 Mutant

Reference: John Wallingford

9/14 NXR_0.0074 Transgenic

Breeding Type: Outbred
Description: 3856bp X. tropicalis Brachyury promoter driving expression of Katushka in muscles post-gastrulation.
Line Type: Transgenic

10/14 NXR_0.0050 Transgenic

Breeding Type: Outbred
Description: RFP fluorescence; YFP or memCFP  fluorescence after Cre-mediated recombination. Germline transmission confirmed.
Line Type: Transgenic
Reference: Nature. 2007 Nov;450:56-62.

12/13 NXR_0.0001 Transgenic

Breeding Type: Outbred
Description: RFP fluorescence; YFP or memCFP  fluorescence after Cre-mediated recombination.
Line Type: Transgenic
Reference: Nature. 2007 Nov;450:56-62.

1/13 & 4/14 NXR_0.0002 Transgenic

Breeding Type: Outbred
Description: RFP fluorescence; YFP or memCFP  fluorescence after Cre-mediated recombination.
Line Type: Transgenic
Reference: Nature. 2007 Nov;450:56-62.

4/14 NXR_0.0081 Transgenic

Breeding Type: Outbred
Description: Whole embryo blue fluorescence; yellow fluorescence upon Cre or FLP recombination.
Line Type: Transgenic
Reference: Nucleic Acids Res. 2003 Apr;31:e44. Dev Dyn. 2006 Aug;235:2220-8.

4/12 NXR_0.0003 Transgenic

Breeding Type: Outbred
Description: Fluorescence detectable in most tissues through development.
Line Type: Transgenic
Reference: Mead Lab

 12/12 NXR_0.0004  Transgenic
CAG-Kikume GR 4/15 NXR_0.0014  Transgenic
Breeding Type: Outbred
Description: YFP fluorescence in most tissues.
Line Type: Transgenic
Reference: Nat Biotechnol. 2002 Jan;20:87-90. Dev Dyn. 2005 Jun;233:562-9.
8/11 NXR_0.0006  Transgenic

Breeding Type: Outbred
Description: Embryonic striated muscle including heart. m
Line Type: Transgenic
Reference: Dev Biol. 2002 May;5:57-70.  Development. 2003 Aug;130(16): 3865-76.

2/12 & 9/14
NXR_0.0007 Transgenic

Reference: Brian Mitchell

8/15 NXR_0.0104 Transgenic

Reference: Rachel Miller

8/15 NXR_0.0102 Transgenic

Reference: Brian Mitchell

7/15 NXR_0.0100 Transgenic

Breeding Type: Outbred
Description: Ubiquitous membrane GFP expression.
Line Type: Transgenic
Reference: Custom transgenic made by the NXR.

4/14  NXR_0.0012 Transgenic

Reference: Andrew Miller

1/14 NXR_0.0063 Transgenic

Reference: Mike Levin

10/14 NXR_0.0092 Transgenic

Breeding Type: Outbred
Description: Whole embryo green fluorescence.
Line Type: Transgenic

8/12 NXR_0.0008 Transgenic

Breeding Type: Outbred
Description: Whole embryo green fluorescence; red fluorescence upon FLP recombination.
Line Type: Transgenic
Reference: Gene. 1996 Nov;180:225-7.   Nucleic Acids Res. 2001 Jun;29:E53-3.  Nucleic Acids Res. 2003 Apr;31:e44.

   1/12
NXR_0.0009  Transgenic

Breeding Type: Outbred
Description: Unbiquitous nuclear RFP expression.
Line Type: Transgenic
Reference: Custom transgenic made by the NXR.

2/15 NXR_0.0094 Transgenic

Reference: Mike Levin

1/15 NXR_0.0095  Transgenic

Breeding Type: Outbred
Description: Chondrocytes.
Line Type: Transgenic
Reference: Int J Dev Biol. 2010;54(1):141-50.

8/11 & 9/15
NXR_0.0010 Transgenic
Reference: Brian Mitchell
8/15 NXR_0.0103 Transgenic
Dria-GFP 1/15 NXR_0.0059 Transgenic

Breeding Type: Outbred
Description: Widespread expression expression apparent from mid-gastrula on.
Line Type: Transgenic
Reference: Gene. 1994 Sep;147:223-6.

11/12 NXR_0.0011 Transgenic

Breeding Type: Outbred
Description: Pancreas. F2 transgenic line.
Line Type: Transgenic
Reference: Mech Dev. 1999 Nov;88:221-7. Development. 2009 Jun;136:1791-800. Dev Biol. 2007 Apr;304(2): 786-99.   Curr Biol. 2003 Jan;13(2):105-15.

9/14 NXR_0.0013 Transgenic

Reference: Hollis Cline

8/15 NXR_0.0107 Transgenic

Breeding Type: Outbred
Description: Floating Head.
Line Type: Transgenic

10/14 NXR_0.0017 Transgenic

Breeding Type: Outbred
Description: Laevis 2.5kb promoter driving GFP expression in vascular endothelial cells.
Line Type: Transgenic
Reference:  Mead Lab. Dev Dyn. 2007 Oct;236:2808-17.

6/13 & 10/14
NXR_0.0018 Transgenic

Breeding Type: Outbred
Description:  2000bp X. tropicalis foxi I promoter driving expression of Katushka in epidermal cells (Ionocytes).
Line Type: Transgenic

8/14 NXR_0.0047 Transgenic
GammaCry-VenusGFP:CMVcentrin 4/15 NXR_0.0096 Transgenic

Breeding Type: Outbred
Description: GATA-1 promoter and first intron driving expression of GFP in GATA-1 expressing cells (red blood cells and various other lineages, such as
Line Type: Transgenic

6/13 NXR_0.0019 Transgenic

Breeding Type: Outbred
Description:
Lenses glow green; heat-shock inducible Noggin.
Line Type:
Transgenic
Reference:
Dev Cell. 2003 Sep;5:429-39. Mech Dev. 2006 Sep;123:674-88.


1/12 & 3/14
NXR_0.0020 Transgenic

Breeding Type: Outbred
Description: Heat-shock inducible Dkk1 to down-regulate Wnt/B-catenin signaling with a Gamma crystallin GFP selection marker.
Line Type: Transgenic
Reference: Dev Biol. 2008 Apr 15;316(2):323-35.

1/14 NXR_0.0021 Transgenic
Insm2 (Cr)
10/14 NXR_0.0091 Mutant

Breeding Type: Outbred
Description: Laevis 5kb insulin promoter driving memGFP expression; gamma crystallin mCherry selection
Line Type: Transgenic
Reference: Custom transgenic made by the NXR

9/13 NXR_0.0056 Transgenic

Breeding Type: J-Strain
Description: Inbred strain; homozygous MHC haplotype; genome sequenced.
Line Type: Wild Type
Reference: Virology. 2003 Jul; 311(2): 254-62.  Dev Growth Differ 1975; 17: 283.

1/13, 5/13, 9/14, 10/14, 11/14, 12/14 NXR_0.0024 Wild Type

Breeding Type: J-Strain
Description: TALEN induced tyrosinase mutant.
Line Type: Mutant
Reference: Custom strain made by the NXR

5/13, 3/14, 5/15
NXR_0.0041 Mutant

Reference: Brian Mitchell

7/15 NXR_0.0099 Transgenic

Breeding Type: Outbred
Description: Embryonic macrophages, definitive myeloid blood cells, restricted neural expression, epidermis after NF49.
Line Type: Transgenic
Reference: Mech Dev. 2002 Sep;117:173-86. Dev Growth Differ. 2006 Oct;48:499-512.

3/12 NXR_0.0025 Transgenic

Breeding Type: Outbred
Description: Heart myocardium.
Line Type: Transgenic
Reference:  Dev Dyn. 2005 Apr;232(4):1003-12.

12/13 NXR_0.0026 Transgenic
MLC2-BicCGFP 12/14 NXR_0.0090 Transgenic

Breeding Type: Outbred
Description:  8kb myosin light chain promoter driving EGFP expression in the ventricular chamber type heart myocardium and interhyoid facial muscle.
Line Type: Transgenic
Reference: Development. 2004 Feb;131(3):669-79.

9/14 NXR_0.0027 Transgenic

Reference: EXRC

7/15 NXR_0.0106  Transgenic[

Reference: Tim Hinteburger

7/13 NXR_0.0043 Mutant

Reference: Tim Hinteburger

8/13 NXR_0.0044 Mutant

Reference: Tim Hinteburger

5/13 NXR_0.0042 Mutant

Reference: Tim Hinteburger

8/13 NXR_0.0054 Mutant

Breeding Type: Outbred
Description: Early heart field, mesoderm, pharyngeal endoderm, spleen.
Line Type: Transgenic
Reference: Dev Biol. 2000 Nov 1;227(1):65-79.

2/12 & 9/14 NXR_0.0030 Transgenic
NXR-WT 8/11, 7/13, 1/14, 5/14, 7/14 & 6/15 NXR_0.0031 Wild Type
Oligo2 KO(Cr) 6/14 NXR_0.0078 Mutant

Breeding Type: Outbred
Description:  2916bp X. laevis Pax3 promoter driving expression of Katushka in the neural ectoderm (neural crest).
Line Type: Transgenic
Reference:  Pax3 promoter from Roberto Mayor (Mauricio Moreno)

9/14 NXR_0.0049 Transgenic
pbin8LEF-dGFP 1/14, 2/15 & 9/15
NXR_0.0064 Transgenic
pHS-bCatenin-GFP:Elas-GFP 6/14 NXR_0.0033 Transgenic
Slc45a2 (Cr/Cas)
10/14 NXR_0.0088 Mutant
Slc45a2-GFP (KI) 12/14 NXR_0.0089 Knock In
Slc5a10 KO (T) 4/14 NXR_0.0073 Mutant

Breeding Type: Outbred
Description: Dorsal mesoderm post-gastrulation, heart endocardium, widespread expression at larval stages.
Line Type: Transgenic
Reference: Mech Dev. 2011 May-Jun;128:303-15.

1/12 NXR_1.0035 Transgenic

Reference: David Furlow

9/14 NXR_0.0085 Mutant
TUBB2-GFP 6/13 & 9/15
NXR_0.0035 Transgenic

Reference: Andrew Miller

12/13 NXR_0.0062 Transgenic
TubulinA1-GFP 6/14 NXR_0.0053 Transgenic

Reference: Mike Zuber

8/14 NXR_0.0083 Mutant

Reference: Richard Harland

7/14 NXR_0.0079 Wild Type

Reference: Richard Harland

7/14 NXR_0.0076 Wild Type
Wild Caught
7/14 NXR_0.0080 Wild Type
Reference: Tim Hinteburger
9/13 NXR_0.0055 Mutant
WT Albino
8/14 NXR_0.0082 Wild Type

Breeding Type: Outbred
Description: Lenses.
Line Type: Transgenic
Reference: Gene. 1993 Jun;128:189-95.

6/11 NXR_0.0036 Transgenic
Xcrys-mCherry:CMV-memGFP 12/12 & 11/13
NXR_0.0038 Transgenic
Xic-GFP 6/14 NXR_0.0051 Transgenic

Breeding Type: Outbred
Description: Rod optsin promoter driving E. coli nitroreductase.
Line Type: Transgenic
Reference: FEBS Lett. 1998 Feb;423:117-21. Invest Ophthalmol Vis Sci. 2011 Jan;52:364-73.

4/12 NXR_0.0067 Transgenic
Breeding Type: Outbred
Description: Whole embryo blue fluorescence; yellow fluorescence upon Cre recombination.
Line Type: Transgenic
Reference: Nucleic Acids Res. 2003 Apr 15;31:e44.

8/12 NXR_0.0040 Transgenic