April 6, 2013

Laevis Stocks

Now accepting orders for custom transgenic Xenopus laevis

 

NXR Frog Sale 

The following lines are currently available for half price:

Xenopus laevis Elastase-GFP

Xenopus laevis Venus YFP

 

Laevis Adult Stocks

Strain Date of Birth Phenotype/Expression Pattern Reference
 Cardiac Actin-GFP 2/2/12  Embryonic striated muscle including heart.  Dev Biol. 2002 May 1;245(1):57-70.Development. 2003 Aug;130(16):3865-76.
 Col2a1-GFP 8/25/11  Chondrocytes. mmmmmmmmmmmmmmmmmmm  Int J Dev Biol. 2010;54(1):141-50.
 CMV-GFP  Whole embryo green fluorescence.   
 CMV-GFP-RFP   1/25/12                 Whole embryo green fluorescence; red fluorescence upon FLP recombination.  Gene. 1996 Nov 21;180(1-2):225-7.Nucleic Acids Res. 2001 Jun 1;29(11):E53-3.Nucleic Acids Res. 2003 Apr 15;31(8):e44.
 Elastase-GFP    Pancreas. mmmmmmmmmmmmmmmmmmmmmm  Mech Dev. 1999 Nov;88(2):221-7.Development. 2009 Jun;136(11):1791-800.Dev Biol. 2007 Apr 15;304(2):786-99.Curr Biol. 2003 Jan 21;13(2):105-15.
 Gamma Crystallin-GFP 6/8/11  Lenses.   Gene. 1993 Jun 30;128(2):189-95.
 J-Line    Inbred strain; genome currently being sequenced.  Virology. 2003 Jul 5;311(2):254-62.; Dev Growth Differ 1975; 17: 283.
 LURP1-GFP 3/21/12  Embryonic macrophages, definitive myeloid blood cells, restricted neural expression, epidermis after NF49.   Mech Dev. 2002 Sep;117(1-2):173-86.Dev Growth Differ. 2006 Oct;48(8):499-   512.
 MCL2-GFP 1/24/12 mmmmmm  Heart myocardium.    Development. 2004 Feb;131(3):669-79.
 NBTtau-GFP   (tubb2-GFP) 1/2/12  Neural.  BMC Dev Biol. 2007 May 31;7:59.Cell. 2008 May 30;133(5):916-27.
 Nkx2.5-GFP 2/2/12  Early heart field, mesoderm, pharyngeal endoderm, spleen.   Dev Biol. 2000 Nov 1;227(1):65-79.
 HGEM-Noggin-GFP 1/26/12  Lenses glow green; heat-shock inducible Noggin.  Dev Cell. 2003 Sep;5(3):429-39.Mech Dev. 2006 Sep;123(9):674-88.
 SCL-GFP mmmmmmmm 3/28/12  Haematopoetic precursors.  EMBO J. 1998 Jul 15;17(14):4029-45.EMBO J. 2002 Jun 17;21(12):3039-50.Mol Cell Biol. 2010 Dec;30(24):5741-51.
 Smad3-GFP   1/19/12              Dorsal mesoderm post-gastrulation, heart endocardium, widespread expression at larval stages.  Mech Dev. 2011 May-Jun;128(5-6):303-15.
 Venus YFP     YFP fluorescence in most tissues.  Nat Biotechnol. 2002 Jan;20(1):87-90.Dev Dyn. 2005 Jun;233(2):562-9.
 Y2   3/14/12                  Whole embryo blue fluorescence; yellow fluorescence upon Cre or FLP recombination.  Nucleic Acids Res. 2003 Apr 15;31(8):e44.

 

Laevis Growing Stocks

Strain  Date of        Birth           Phenotype/Expression Pattern Reference
 CMV-Brainbow-1.0 H 12/5/12  RFP fluorescence; YFP or CFP  fluorescence after Cre-mediated recombination.  Nature. 2007 Nov 1;450(7166):56-62.
 CMV Brainbow-1.0 L 1/22/13   RFP fluorescence; YFP or CFP  fluorescence after Cre-mediated recombination.  Nature. 2007 Nov 1;450(7166):56-62.
 CAG-GFP 12/3/12  Fluorescence detectable in most tissues through development.  Mead Lab
 C5 4/10/12  Whole embryo blue fluorescence; yellow  fluorescence upon Cre or FLP recombination.  Nucleic Acids Res. 2003 Apr 15;31(8):e44.Dev Dyn. 2006 Aug;235(8):2220-8.
 CMV-memGFP (Cry mCherry) 12/12/12  Ubiquitous membrane GFP expression.  Custom transgenic made by the NXR.
 CMV-H2BmRFP (Cry Venus) 2/6/13  Unbiquitous nuclear RFP expression.  Custom transgenic made by the NXR.
 Ef1a-GFP 11/30/12  Widespread expression expression apparent from mid-gastrula on.  Gene. 1994 Sep 30;147(2):223-6.
 J-Line 5/22/12  Inbred strain; genome currently being sequenced. mmmmmmmmmmmmmmmmmm  
 PCR-XOP-GFP 4/17/12  Rod photoreceptors.  FEBS Lett. 1998 Feb 20;423(2):117-21.Invest Ophthalmol Vis Sci. 2011 Jan 21;52(1):364-73.

 

For detailed information on specific lines or to request frogs, please email Xenopus@mbl.edu.