July 28, 2014

Laevis Stocks

For detailed information on specific lines or to request frogs, please email Xenopus@mbl.edu.

 

Now accepting orders for custom transgenic or TALEN Xenopus laevis lines
NXR Custom Transgenics Information

 

 

The following lines are currently available for half price:

Xenopus laevis Elastase-GFP

 

 

Mem-GFP Transgenic

Mem-GFP Transgenic

NBT-GFP (Tubb2) Transgenic

NBT-GFP (Tubb2) Transgenic

 

 

 

 

 

 

 

 

 

 

 

Laevis Adult Stocks

Strain Date of Birth Genetic Background Phenotype/Expression Pattern Xenbase Reference# Reference
 Cardiac Actin-GFP 2/12 Nasco outbred  Embryonic striated muscle including heart. m Dev Biol. 2002 May;5:57-70.Development. 2003 Aug;130:3865-76.
 CAG-GFP 12/12 Nasco outbred  Fluorescence detectable in most tissues through development.   Mead Lab
 Col2a1-GFP mmmmmmmmmmmmm 8/11 Nasco outbred  Chondrocytes. 
 Int J Dev Biol. 2010;54:141-50.
 CMV-GFP 8/12  Nasco outbred  Whole embryo green fluorescence.  mmmmmmmmmmmmmmmmmmmmmmmmmmmm
CMV-GFP-RFP 1/12 Nasco outbred  Whole embryo green fluorescence; red fluorescence upon FLP recombination. Gene. 1996 Nov;180:225-7.Nucleic Acids Res. 2001 Jun;29:E53-3.Nucleic Acids Res. 2003 Apr;31:e44.
 CMV-eCFP(loxP)(FRT)eYFP  (C5) 4/12 Nasco outbred  Whole embryo blue fluorescence; yellow fluorescence upon Cre or FLP recombination.  Nucleic Acids Res. 2003 Apr;31:e44.Dev Dyn. 2006 Aug;235:2220-8.
  CMV-eCFP(loxP)eYFP (Y2) 3/12 Nasco outbred Whole embryo blue fluorescence; yellow fluorescence upon Cre recombination. Nucleic Acids Res. 2003 Apr 15;31:e44.
 Gamma Crystallin-GFP 6/11  Nasco outbred  Lenses.
Gene. 1993 Jun;128:189-95.
 EF1a-GFP 11/12 Nasco outbred  Widespread expression expression apparent from mid-gastrula on.   Gene. 1994 Sep;147:223-6.
 Elastase-GFP  10/11 Nasco outbred  Pancreas. F2 transgenic line. Mech Dev. 1999 Nov;88:221-7.Development. 2009 Jun;136:1791-800.Dev Biol. 2007 Apr;304:786-99.Curr Biol. 2003 Jan;13:105-15.
 J strain  3/11, 5/12 J strain inbred  Inbred strain; homozygous MHC haplotype; genome sequenced. Virology. 2003 Jul;311:254-62.Dev Growth Differ 1975; 17: 283.
 LURP1-GFP 3/12 Nasco outbred  Embryonic macrophages, definitive myeloid blood cells, restricted neural expression, epidermis after NF49. Mech Dev. 2002 Sep;117:173-86.Dev Growth Differ. 2006 Oct;48:499-512.
 MCL2-GFP 1/12 mmmmmm Nasco outbred  Heart myocardium.  Development. 2004 Feb;131(3):669-79.
 Tubb2-GFP (NBTtau-GFP) 1/12 Nasco outbred  GFP expression in the neural tissues. BMC Dev Biol. 2007 May;7:59.Cell. 2008 May 30;133(5):916-27.
 Nkx2.5-GFP 2/12 Nasco outbred  Early heart field, mesoderm, pharyngeal endoderm, spleen. Dev Biol. 2000 Nov 1;227(1):65-79.
 HGEM-Noggin-GFP 1/12 Nasco outbred  Lenses glow green; heat-shock inducible Noggin. Dev Cell. 2003 Sep;5:429-39.Mech Dev. 2006 Sep;123:674-88.
 XOP-NTR 4/12 Nasco outbred  Rod optsin promoter driving E. coli nitroreductase. FEBS Lett. 1998 Feb;423:117-21.Invest Ophthalmol Vis Sci. 2011 Jan;52:364-73.
 SCL-GFP mmmmmmmm 3/12 Nasco outbred  GFP expression in haematopoetic precursors. EMBO J. 1998 Jul;17:4029-45.EMBO J. 2002 Jun;21:3039-50.Mol Cell Biol. 2010 Dec;30:5741-51.
 Smad3-GFP       1/12             Nasco outbred  Dorsal mesoderm post-gastrulation, heart endocardium, widespread expression at larval stages. Mech Dev. 2011 May-Jun;128:303-15.
 Venus YFP  8/11  Nasco outbred  YFP fluorescence in most tissues. Nat Biotechnol. 2002 Jan;20:87-90.Dev Dyn. 2005 Jun;233:562-9.
 Wild type 8/11, 7/13, 1/14 Nasco outbred  Traditional wild type.

 

 

Laevis Germline Confirmed F1 Growing Stocks 

Strain  Date of        Birth           Genetic Background  Phenotype/Expression Pattern Xenbase Reference# Reference
 CMV-Brainbow-1.0 H 12/13 Nasco outbred  RFP fluorescence; YFP or memCFP  fluorescence after Cre-mediated recombination. Germline transmission confirmed.  Nature. 2007 Nov;450:56-62.
 CMV-Brainbow-1.0 L 5/14 Nasco outbred  RFP fluorescence; YFP or memCFP  fluorescence after Cre-mediated recombination.  Nature. 2007 Nov;450:56-62.
 CMV-memGFP (Cry mCherry) mmmmmmmmmmm 12/13 Nasco outbred  Ubiquitous membrane GFP expression.  Custom transgenic made by the NXR.
 CMV-H2BmRFP (Cry Venus) mmmmmmmmmm 2/14 Nasco outbred  Unbiquitous nuclear RFP expression. mmmmmmmmmmmmm Custom transgenic made by the NXR.
 HGEM-Dkk1 1/14 Nasco outbred  Heat-shock inducible Dkk1 to down-regulate Wnt/B-catenin signaling with a Gamma crystallin GFP selection marker.  Dev Biol. 2008 Apr 15;316(2):323-35.
 Flk1-GFP 6/13  Nasco outbred  Laevis 2.5kb promoter driving GFP expression in vascular endothelial cells.  Mead Lab. Dev Dyn. 2007 Oct;236:2808-17. mmmmmmmmmmmmmmmmmmm
 Gata1-GFP mmmmmmm 6/13 Nasco outbred
 J strain 12/13 J strain inbred  Inbred strain; homozygous MHC haplotype; genome sequenced.
 Virology. 2003 Jul;311:254-62.Dev Growth Differ 1975; 17: 283.
 Tubb2-GFP (NBTtau-GFP) 2/14 Nasco outbred  GFP expression in the neural tissues.  BMC Dev Biol. 2007 May;7:59.Cell. 2008 May;133:916-27.
 MLC1V-eGFP 12/13 Nasco outbred  8kb myosin light chain promoter driving EGFP expression in the ventricular chamber type heart myocardium and interhyoid facial muscle.  Dev Dyn. 2005 Apr;232:1003-12.
 Wnt Reporter mmmmmm 1/14 Nasco outbred  Wnt reporter line.  PNAS. 2010 Sep;107:16160-5.

 

 

Laevis F0 Growing Stocks

Strain Date of Birth Genetic Background Phenotype/Expression Pattern Xenbase Reference# Reference
 Insulin-memGFP (Cry mCherry) mmmmmmmmm 9/13 Nasco outbred Laevis 5kb insulin promoter driving memGFP expression; gamma crystallin mCherry selection Custom transgenic made by the NXR
 J strain albino 5/13 J strain inbred TALEN induced tyrosinase mutant. Custom strain made by the NXR
 Tubb2-mCherry (NBT-mCherry) 2/14 Nasco outbred RFP expression in the neural tissues. Custom transgenic made by the NXR
 MBP-memGFP (Cry mCherry) 2/14 Nasco outbred  Myelin basic protein promoter driving expression of GFP in oligodendrocytes.  J Neurosci. 2012 Sep;32:12885-95.