Tropicalis Stocks

For detailed information on specific lines or to request frogs, please email Xenopus@mbl.edu.

Now accepting orders for custom transgenic or mutant Xenopus tropicalis lines
NXR Custom Transgenics

Tropicalis Adult Stocks

Source: EXRC

Strains Image
Xtr.Ivory-CoastNXR

NXR_1.0009

Description: F4 Ivory Coast ‘blue’ frogs.

Reference:

Xtr.NigerianSt549

NXR_1.0018

Description: (Nigerian St.549)

Reference:

Transgenic Line Image
Xtr.Tg(CAGGS-SB10,cryga-RFP)4MMead

NXR_1.0001

Description: (4MHoppers)

Reference: Mead

Xtr.Et(CAGGS-SB10,cryga-RFP)622EMead

NXR_1.0002

Description: (622EHoppers)

Reference: Mead

622e_sb111

Source: NXR

Xtr.Et(CAGGS=SB10,cryga-RFP)7M8FMead

NXR_1.0003

Description: (7M8FHoppers)

Reference: Mead

Xtr.Tg(CAGGS-SB10,cryga-RFP)7MMead

NXR_1.0004

Description: (7MHoppers)

Reference: Mead

Xtr.Et(CAGGS-SB10,cryga-RFP)8FMead

NXR_1.0005

Description: (8FHoppers)

Reference: Mead

Xtr.Tg(CMV:memGFP)NXR

NXR_1.0007

Description: (CMVmemGFP) CMV driving ubiquitous expression of membrane GFP

Reference: Custom line generated by the NXR.

cmvmemgfp

Source: NXR

Source: NXR

Source: NXR

Xtr.Tg(CMV-Aequorin-GFP)NXR

NXR_1.0070

Description: (CMV-Aequorin-GFP)

Reference: Custom line generated by NXR for Andrew Miller.

Xtr.Tg(eef1a1:GFP)Krieg

NXR_1.0008

Description: (Ef1a-GFP)Widespread expression apparent from mid-gastrula on.

Reference: Gene. 1994 Sep 30;147(2):223-6. Xenbase.

Xtr.Et(gata1:GFP)mysMead

NXR_1.0012

Description: (mTol2G1GFP-M4msy) GFP expression in the venous vasculature; integration near the 5′ UTR of the apelin receptor gene.

Reference: Mead Lab; Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Xtr.Et(krt8:GAL4-VP16,UAS:GFP)Mead

NXR_1.0014

Description: (mTol2k8GAL4) keratin 8 minimal promoter driving GAL4VP16 fusion with a uAS GFP reporter gene. Krt8 promoter weakly expression in skin = good for enhancer trapping.

Reference: Mead Lab; Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11

Xtr.Tg(Ntub GalPR BicB)

NXR_1.0109

Description: (Ntub GalPR BicB) Ntub Galpr Bic- N-tubulin promoter driving Gal4Pr (RU482-inducible Gal4-progesterone receptor VP16 fusion) in post-mitotic neurons. Cry-CFP marker cassette (linked)

Reference: EXRC.

Xtr.Tg(NBT-PLAPxcryRFP)Amaya

NXR_1.0099

Description: (NBT-PLAPxcryRFP)

Reference: EXRC. BMC Devlp. Bio. 2007 May; 7:59.

Xtr.Tg(otx2:GFP)Kellr

NXR_1.0020

Description: (Otx2-GFP) Forebrain/midbrain

Reference:  Development. 1995 Apr; 121(4): 993-1004. Development. 1995 Mar; 121(3): 707-20. Xenbase.

Source: NXR

Source: NXR

otx2gfp_1

Source: NXR

Xtr.Tg(Pax6:GFP)

NXR_1.0107

Description: (Pax6-GFP)

Reference: EXRC. Xenbase.

pax6-gfp

Source: NXR

Source: NXR

Source: NXR

Xtr.Tg(Pax6galPRBicolorBlue)

NXR_1.0098

Description: (Pax6galPRBicolorBlue) Pax6 promoter driving RU486-inducible progesterone receptor/VP16/Gal4 transactivator, in transgene marked by g-cry ECFP cassette. ECFP in lens of eye; inducible Gal4 drives expression of UAS-transgenes in the presence of RU486 in the eye and dorsal CNS. Labels lens of eye; other functions depend on separate effector UAS transgenes

Reference: EXRC.

Xtr.Tg(pax6:GFP,cryga:RFP,actc1:RFP)Papal

NXR_1.0021

Description: (Pax6-GFP:xcryFRP:CardiacActin-RFP) Green eyes, brain, and spinal cord, beginning neural tube stage; yellow lenses from talibud stages on; red muscle from talibud stages on.

Reference:  Dev Biol. 2001 Oct 1; 238(1): 168-84. Dev. Dyn. 2002 Dec; 225(4): 552-35.

pax6tripletrans_1

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Xtr.Tg(pbin7LEF-GFP)

NXR_1.0094

Description: (pbin7LEF-GFP/Wnt Reporter)

Reference: EXRC.

Source: EXRC

Source: EXRC

pbin7lefgfp

Source: NXR

Xtr.Et(CAGGS-SB10,cryga-RFP); Tg(CAGGS:GFP)6MMead

NXR_1.0026

Description: (pT2BGFP-6M) Widespread GFP fluorescence.

Reference: Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.

Xtr.Et(CAGGS-SB10,cryga-RFP); Tg(CAGGS:GFP)622EMead

NXR_1.0025

Description: (pT2BGFP-622E) Widespread GFP fluorescence: outcross of the ‘hopper’ frogs will result in remobilization of the substate transposon and re-integration elsewhere in the genome resulting in expression pattern and/or intensity of GFP expression.

Reference: Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.

Xtr.Et(CAGGS-SB10,cryga-RFP); Tg(CAGGS:GFP)8FMead

NXR_1.0028

Description: (pT2BGFP-8F) LDouble transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events.

Reference: Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.

Xtr.Tg(SB10enzyme)Mead

NXR_1.0034

Description: (SB10enzyme)

Reference: Mead

Xtr.Et(eef1a1:GFP)Hbr,Cen,Fol,BruMead

NXR_1.0037

Description: (Tol2X1G-12MHbrCenFolBru)

Reference: Mead

Xtr.Et(eef1a1:GFP)folMead

NXR_1.0083

Description: (Tol2X1G-12Mfol)

Reference:  Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Xtr.Et(eef1a1:GFP)cenMead

NXR_1.0040

Description: (Tol2X1G-Mcen) Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the brain.

Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Xtr.Et(eef1a1:GFP)grbMead

NXR_1.0042

Description:  (Tol2X1G-12Mgrb) Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the brain.

Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Xtr.Et(eef1a1:GFP)hbrMead

NXR_1.0043

Description: (Tol2X1G-12Mhbr)Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP.

Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Xtr.Et(eef1a1:GFP)johMead

NXR_1.0044

Description: (Tol2X1G-12Mjoh) Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the kidney, integration near the HNF1b gene.

Reference:  Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Xtr.Et(eef1a1:GFP)psvbiMead

NXR_1.0046

Description: (Tol2X1G-16Fpsvbi)

Reference: Mead Xenbase.

Xtr.Et(eef1a1:GFP)16FMead

NXR_1.0047

Description: (Tol2X1G-16Fubiq) Ubiquitous integration of EF1a promoter driving GFP expression.

Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Xtr.Tg(TTR-Chibby)

NXR_1.0066

Description: (TTR-Chibby)

Reference:

Xtr.Tg(Tubb2-GFP)

NXR_1.0092

Description: (NBT/Tubb2-GFP)

Reference: EXRC. Xenbase.

Source: EXRC

Source: EXRC

Xtr.Tg(UbiC-membrane Venus)

NXR_1.0106

Description: (UbiC-membraneVenus)

Reference: EXRC

ubiqmemvenus

Source: NXR

Xtr.Tg(UbiC Flox RFPGFP)

NXR_1.0108

Description: Ubiquitous expression, floxed RFP GFP reporter. Ubiquitously expressed RFP; upon cre-mediated recombination expresses GFP and loses RFP.

Reference: Transgenic generated by Bettina Ryll. EXRC.

Xtr.Tg(Ubiq Gal4,cryRFP)

NXR_1.0110

Description: (UbiqGal4,cryRFP)

Reference: EXRC.

Xtr.Tg(cryga:GFP)Grainger

NXR_1.0049

Description: (xCrys-GFP)

Reference: Grainger. Xenbase.

Xtr.Tg(yCry-RFP-Vimentin GFP)

NXR_1.0093

Description: (yCry-RFP:Vimentin-GFP)

Reference: EXRC. Xenbase.

Source: NXR

Source: NXR

xcryrfpvimentingfp_1

Source: NXR

Mutant Line Image
noprivacy

NXR_1.0019

Description: (noprivacy) Characteristic of albinos through froglet stage; slight pigmentation as adults.

Reference: Grainger

Raxhet

NXR_1.0033

Description: (Raxhet) Presumptive retina and forebrain

Reference:  Nature. 1997 Jun 5;387(6633):603-7. Dev Dyn. 2002 Dec;225(4):522-35. Xenbase.

Xtr.tyrtmNXR

NXR_1.0069

Description: (Tyrosinanse KO(Cr))

Reference: Custom mutant generated by the NXR. Xenbase.

 snap-103955-0040snap-103803-0026 snap-103048-0014
Xtr.map3k13tmHorb

NXR_3.0017

Description: CRISPR knockout of map3k13, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.aif1ltmHorb

NXR_3.0005

Description: CRISPR knockout of aif1l, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.ascl1tmHorb

NXR_3.0018

Description: CRISPR knockout of ascl1, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.erftmHorb

NXR_3.0004

Description: CRISPR knockout of erf, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

 

Xtr.foxc2tmHorb

NXR_3.0002

Description: CRISPR knockout of foxc2, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.frzb,frzb2tmHorb

NXR_3.0020

Description: CRISPR knockout of fzrb and fzrb2, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase Frzb. Xenbase Frzb2.

Xtr.glis3tmHorb

NXR_3.0011

Description: CRISPR knockout of glis3, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.hpse2tmHorb

NXR_3.0010

Description: CRISPR knockout of hpse2, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.insm2tmHorb

NXR_3.0014

Description: CRISPR knockout of insm2, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.lmnatmHorb

NXR_3.0007

Description: CRISPR knockout of lmna, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.mitftmHorb

NXR_3.0001

Description: CRISPR knockout of mitf, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.olig2tmHorb

NXR_3.0006

Description: CRISPR knockout of olig2, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.olig4tmHorb

NXR_3.0019

Description: CRISPR knockout of olig4, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.pou3f4tmHorb

NXR_3.0015

Description: CRISPR knockout of pou3f4, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.prph2tmHorb

NXR_3.0003

Description: CRISPR knockout of prph2, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.runx1tmHorb

NXR_3.0016

Description: CRISPR knockout of runx1, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.rxrbtmHorb

NXR_3.0012

Description: CRISPR knockout of rxrb, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.rxrgtmHorb

NXR_3.0013

Description: CRISPR knockout of rxrg, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.tlx3tmHorb

NXR_3.0008

Description: CRISPR knockout of tlx3, Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.

Xtr.gdf1tmHorb

NXR_3.0009

Description: CRISPR knockout of gdf1(vg1), Current mosaic founders, Not tested for germline transmission.

Reference: Custom mutant generated by Horb Lab. Xenbase.