Tropicalis Stocks

For detailed information on specific lines or to request frogs, please email Xenopus@mbl.edu.

Now accepting orders for custom transgenic or TALEN Xenopus tropicalis lines
NXR Custom Transgenics

Tropicalis Adult Stocks

Strain Date of Birth Catalog  Number
4MHoppers 11/12 & 5/13
NXR_1.0001
622EHoppers 11/12 & 5/13
NXR_1.0002
7M8FHoppers 5/13 NXR_1.0003
7MHoppers 5/13 NXR_1.0004
8FHoppers 8/13 NXR_1.0005
Chibby KO(T) 1/14
NXR_1.0068
Description: CMV driving ubiquitous expression of membrane GFP
Line Type: Transgenic
Reference: Custom strain made by the NXR.
4/14 NXR_1.0007
CMV-Aequorin-GFP 1/14
NXR_1.0070

Breeding Type: Inbred
Description: Inbred strain of WT Nigerian frogs.
Line Type: Wild Type

1/14, 4/15, 5/15, & 6/15
 NXR_1.0090

Description: Widespread expression apparent from mid-gastrula on.
Line Type: Transgenic
Reference: Gene. 1994 Sep 30;147(2):223-6.

3/11 & 3/15
NXR_1.0008

Breeding Type: Inbred
Description: F4 Ivory Coast ‘blue’ frogs.
Line Type: Wild Type

   3/11, 3/15, & 6/15
NXR_1.0009

Description: GFP expression in the venous vasculature; integration near the 5′ UTR of the apelin receptor gene.
Line Type: Transgenic
Reference: Mead Lab; Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11

8/13 NXR_1.0012

Description: keratin 8 minimal promoter driving GAL4VP16 fusion with a uAS GFP reporter gene. Krt8 promoter weakly expression in skin = good for enhancer trapping.
Line Type: Transgenic
Reference: Mead Lab; Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11

4/13 NXR_1.0014
Myf6 KO(T)
10/13 NXR_1.0067
NigerianSt549 10/13, 1/14, 6/14, 1/15, 2/15, 4/15, 6/15, & 7/15
NXR_1.0018

Description: Characteristic of albinos through froglet stage; slight pigmentation as adults.
Line Type: Mutant

2/15 NXR_1.0019
Olig4 KO(T)
8/13 & 12/14
NXR_1.0064

Description: Forebrain/midbrain
Line Type: Transgenic
Reference:  Development. 1995 Apr; 121(4): 993-1004. Development. 1995 Mar; 121(3): 707-20.

6/11
NXR_1.0020

Description: Green eyes, brain, and spinal cord, beginning neural tube stage; yellow lenses from talibud stages on; red muscle from talibud stages on.
Line Type: Transgenic
Reference:  Dev Biol. 2001 Oct 1; 238(1): 168-84. Dev. Dyn. 2002 Dec; 225(4): 552-35.

2/11 NXR_1.0021

Description: Widespread GFP fluorescence.
Line Type: Transgenic
Reference: Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.

8/13 NXR_1.0026

Description: Widespread GFP fluorescence: outcross of the ‘hopper’ frogs will result in remobilization of the substate transposon and re-integration elsewhere in the genome resulting in expression pattern and/or intensity of GFP expression.
Line Type: Transgenic
Reference: Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.

6/13 NXR_1.0025

Description: LDouble transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. 
Line Type:
Transgenic
Reference:
Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.


1/13
NXR_1.0028

Description: Presumptive retina and forebrain
Line Type: Mutant
Reference:  Nature. 1997 Jun 5;387(6633):603-7. Dev Dyn. 2002 Dec;225(4):522-35.

5/11 NXR_1.0033
SB10enzyme
4/14 NXR_1.0034
Six1 KO(Cr)
12/14 & 5/15
NXR_1.0089
Superman 7/13, 9/14, 12/14, 3/15, & 7/15
NXR_1.0049
Tol2X1G-12MHbrCenFolBru 5/13 NXR_1.0037

Line Type: Transgenic
Reference:  Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

4/14 NXR_1.0038

Description:  Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the brain.
Line Type: Transgenic
Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

5/13 NXR_1.0040

Description:  Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the brain.
Line Type: Transgenic
Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

4/13 NXR_1.0042

Description: Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP.
Line Type: Transgenic
Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

8/13 NXR_1.0043

Description: Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the kidney, integration near the HNF1b gene.
Line Type: Transgenic
Reference:  Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

5/13 NXR_1.0044
Tol2XlG-16Fpsvbi 4/14
NXR_1.0046

Description: Ubiquitous integration of EF1a promoter driving GFP expression.
Line Type: Transgenic
Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

4/13 NXR_1.0047
TTR-Chibby 8/13 NXR_1.0066
Tyrosinase KO(Cr)
1/14 & 7/14
NXR_1.0069
Xcrys-GFP 4/12 NXR_1.0049