Tropicalis Stocks

For detailed information on specific lines or to request frogs, please email Xenopus@mbl.edu.

Now accepting orders for custom transgenic or mutant Xenopus tropicalis lines
NXR Custom Transgenics

Tropicalis Adult Stocks

Strain Catalog  Number
4MHoppers NXR_1.0001
622EHoppers NXR_1.0002
7M8FHoppers NXR_1.0003
7MHoppers NXR_1.0004
8FHoppers NXR_1.0005
Description: CMV driving ubiquitous expression of membrane GFP
Line Type: Transgenic
Reference: Custom strain made by the NXR.
NXR_1.0007
CMV-Aequorin-GFP NXR_1.0070

Description: Widespread expression apparent from mid-gastrula on.
Line Type: Transgenic
Reference: Gene. 1994 Sep 30;147(2):223-6.

NXR_1.0008

Breeding Type: Inbred
Description: F4 Ivory Coast ‘blue’ frogs.
Line Type: Wild Type

NXR_1.0009

Description: GFP expression in the venous vasculature; integration near the 5′ UTR of the apelin receptor gene.
Line Type: Transgenic
Reference: Mead Lab; Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11

NXR_1.0012

Description: keratin 8 minimal promoter driving GAL4VP16 fusion with a uAS GFP reporter gene. Krt8 promoter weakly expression in skin = good for enhancer trapping.
Line Type: Transgenic
Reference: Mead Lab; Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11

NXR_1.0014

Line Type: Transgenic
Reference: EXRC

NXR_1.0099
NigerianSt549 NXR_1.0018

Description: Characteristic of albinos through froglet stage; slight pigmentation as adults.
Line Type: Mutant

NXR_1.0019

Description: Forebrain/midbrain
Line Type: Transgenic
Reference:  Development. 1995 Apr; 121(4): 993-1004. Development. 1995 Mar; 121(3): 707-20.

NXR_1.0020

Line Type: Transgenic
Reference:
EXRC

NXR_1.0098

Description: Green eyes, brain, and spinal cord, beginning neural tube stage; yellow lenses from talibud stages on; red muscle from talibud stages on.
Line Type: Transgenic
Reference:  Dev Biol. 2001 Oct 1; 238(1): 168-84. Dev. Dyn. 2002 Dec; 225(4): 552-35.

NXR_1.0021

Reference: EXRC

NXR_1.0094

Description: Widespread GFP fluorescence.
Line Type: Transgenic
Reference: Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.

NXR_1.0026

Description: Widespread GFP fluorescence: outcross of the ‘hopper’ frogs will result in remobilization of the substate transposon and re-integration elsewhere in the genome resulting in expression pattern and/or intensity of GFP expression.
Line Type: Transgenic
Reference: Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.

NXR_1.0025

Description: LDouble transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. 
Line Type:
Transgenic
Reference:
Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.


NXR_1.0028

Description: Presumptive retina and forebrain
Line Type: Mutant
Reference:  Nature. 1997 Jun 5;387(6633):603-7. Dev Dyn. 2002 Dec;225(4):522-35.

NXR_1.0033
SB10enzyme
NXR_1.0034
Tol2X1G-12MHbrCenFolBru NXR_1.0037

Line Type: Transgenic
Reference:  Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

NXR_1.0038

Description:  Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the brain.
Line Type: Transgenic
Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

NXR_1.0040

Description:  Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the brain.
Line Type: Transgenic
Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

NXR_1.0042

Description: Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP.
Line Type: Transgenic
Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

NXR_1.0043

Description: Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the kidney, integration near the HNF1b gene.
Line Type: Transgenic
Reference:  Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

NXR_1.0044
Tol2XlG-16Fpsvbi NXR_1.0046

Description: Ubiquitous integration of EF1a promoter driving GFP expression.
Line Type: Transgenic
Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11.

NXR_1.0047
TTR-Chibby NXR_1.0066

Reference: EXRC

NXR_1.0092
Tyrosinase KO(Cr)
NXR_1.0069
Xcrys-GFP NXR_1.0049
Reference: EXRC
NXR_1.0093