July 25, 2014

Tropicalis Stocks

For detailed information on specific lines or to request frogs, please email Xenopus@mbl.edu.

 

Now accepting orders for custom transgenic or TALEN Xenopus tropicalis lines
NXR Custom Transgenics

 

 Tropicalis Adult Stocks

Strain Date of Birth Genetic Background Phenotype/Expression Pattern Xenbase Reference#  Reference
 Browny 6/11  Less pigmented eggs; adults fully pigmented similar to WT.  
 Ivory Coast WT (C731) 3/11 Ivory Coast  F4 Ivory Coast ‘blue’ frogs. mmmmmmmmmmm  Ivory Coast Inbred Line
 EF1a-GFP 3/11  Widespread expression expression apparent from mid-gastrula on.  Gene. 1994 Sep 30;147(2):223-6.
 Gamma Crystallin-GFP      10/03  GFP expression in the lens.  
 Nigerian WT Inbred 1/11 Nigerian  Inbred strain of WT Nigerian frogs.  
 No Privacy-Heterozyg. 10/07  Characteristic of abinos through froglet stage; slight pigmentation as adults.  
 No Privacy-Homozyg. 5/11  Characteristic of abinos through froglet stage; slight pigmentation as adults.  
 Otx2-GFP 5/11  Forebrain/midbrain.  Development. 1995 Apr;121(4):993-1004.Development. 1995 Mar;121(3):707-20.
 Pax6-GFP, Cry-RFP, Cardiac Actin-RFP 2/11  Green eyes, brain and spinal cord beginning neural tube stage; yellow lenses from tailbud stages on; red muscle from tailbud stages on.  Dev Biol. 2001 Oct 1;238(1):168-84.Dev Dyn. 2002 Dec;225(4):522-35.
 PopA 4/04 mmmmm   mmmmmmmmmmmmmmmmmmmmmmmm  
 Rax mutant 5/11  Presumptive retina and forebrain.  Nature. 1997 Jun 5;387(6633):603-7.Dev Dyn. 2002 Dec;225(4):522-35.
pT2BGFP 8F Hoppers  8/13  Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events.  Mead Lab; Dev Dyn. 2009 Jul;238(7):1727-43. doi: 10.1002/dvdy.21994.Mob DNA. 2011 Nov 24;2:15. doi: 10.1186/1759-8753-2-15.
 pT2BGFP 622E 5/13  Widespread GFP fluorescence: outcross of the ‘hopper’ frogs will result in remobilization of the substrate transposon and re-integration elsewhere in the genome resulting in changes in expression pattern and/or intensity of GFP expression.  Mead Lab; Dev Dyn. 2009 Jul;238(7):1727-43. doi: 10.1002/dvdy.21994.Mob DNA. 2011 Nov 24;2:15. doi: 10.1186/1759-8753-2-15.
  pT2BGFP 7M8F 5/13   Widespread GFP fluorescence: outcross of the ‘hopper’ frogs will result in remobilization of the substrate transposon and re-integration elsewhere in the genome resulting in changes in expression pattern and/or intensity of GFP expression.    Mead Lab; Dev Dyn. 2009 Jul;238(7):1727-43. doi: 10.1002/dvdy.21994.Mob DNA. 2011 Nov 24;2:15. doi: 10.1186/1759-8753-2-15.
 pT2BGFP 6M 8/13  Widespread GFP fluorescence.  Mead Lab; Dev Dyn. 2009 Jul;238(7):1727-43. doi: 10.1002/dvdy.21994.Mob DNA. 2011 Nov 24;2:15. doi: 10.1186/1759-8753-2-15.
 Tol1G hbr/cen/fol/bru 5/13  Mead Lab; Genesis. 2006 Sep;44(9):438-45.BMC Dev Biol. 2010 Jan 22;10:11.
 Tol2XIG Centaur (cen) 5/13  Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the brain.   Mead Lab; Genesis. 2006 Sep;44(9):438-45.BMC Dev Biol. 2010 Jan 22;10:11.
 Tol2XIG Garibaldi (gbr) 4/13  Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. Integration into the 3’’ UTR of smad7 gene.  Mead Lab; Genesis. 2006 Sep;44(9):438-45.BMC Dev Biol. 2010 Jan 22;10:11. 
 Tol2XIG Handlebar (hbr) 8/13  Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP.  Mead Lab; Genesis. 2006 Sep;44(9):438-45.BMC Dev Biol. 2010 Jan 22;10:11.
 Tol2XIG Jovan Heat (joh) 5/13  Tol2 transposon harboring a Xenopus EF1a promoter driving expression of GFP. GFP expression in the kidney; integration near the HNF1b gene.  Mead Lab; Genesis. 2006 Sep;44(9):438-45.BMC Dev Biol. 2010 Jan 22;10:11.
 Tol2XIG 16F ubiq 4/13  Ubiquitous integration of Ef1a promoter driving GFP expression.  Mead Lab; Genesis. 2006 Sep;44(9):438-45.BMC Dev Biol. 2010 Jan 22;10:11.
 mTol2 Gata1 GFP Mister Stripey (msy) 8/13  GFP expression in the venous vasculature; integration near the 5′ UTR of the apelin receptor gene.   Mead Lab; Genesis. 2006 Sep;44(9):438-45.BMC Dev Biol. 2010 Jan 22;10:11. 
 mTol2 K8 Gal4 VP16uasGFP 4/13 Keratin 8 minimal promoter driving driving GAL4VP16 fusion with a uAS GFP reporter gene. Krt8 promoter weakly expression in skin = good for enhancer trapping.  Mead Lab; Genesis. 2006 Sep;44(9):438-45.BMC Dev Biol. 2010 Jan 22;10:11. 

 

Tropicalis Growing Stocks (Germline transmission not yet confirmed)

Strain Date of Birth Genetic Background Phenotype/Expression Pattern Xenbase Reference# Reference
 CMV-memGFP 4/14 Nigerian  CMV driving ubiquitous expression of membrane GFP  Custom strain made by NXR.
 Nigerian WT Albino 2/14 Nigerian  CRISPR-induced tyrosinase mutant.  Custom strain made by NXR.
 Nigerian WT Inbred  1/14, 4/14, 6/14  Nigerian  Inbred strain of WT Nigerian frogs.   
 Tubb2-memGFP (NBT-memGFP) 5/14 Nigerian  Tubb2 promoter driving membrane GFP expression in the neural tissues.  Custom strain made by NXR.

 

New Tropicalis Lines in Progress

Brainbow (4 unique lines)

CMV-H2BmRFP (Gamma Crys Venus selection marker)

CMV-EB3-GFP (Gamma Crys mCherry selection marker)

Myelin Basic Protein (MBP)-memGFP (Gamma Crys mCherry selection marker)

 

 

For detailed information on specific lines or to request frogs, please email Xenopus@mbl.edu.