Genome Editing

Xenopus Genome Editing Workshop

at the Marine Biological Laboratory
Woods Hole, MA

Next Workshop: August 28th – September 3rd, 2017
Syllabus (PDF Format)  

 

Workshop Cost:
Event housing, meals and registration, Single room – $2,200
Event housing, meals and registration, Double room -$1,800
Event meals and registration (no housing)    –         $1,400

 

Included in the cost are essential lab consumables and frogs, including designing and cloning sgRNA in advance or TALEN pairs specific for your own research. Since the goal of the workshop is to learn the techniques and procedures, it is not imperative that you actually create a line during the 5 days.

 

Topics covered include:
Theoretical and practical aspects of CRISPR/Cas9 and TALENs
Methods for genotyping (DSP, HRMA, T7E1, and others)
Maximizing target site choice and sgRNA design
Applications for the maternal host transfer technique combined with genome editing
Gene targeting and homology-directed repair
Circumventing lethal phenotypes
Xenopus genetics and husbandry issues arising in the creation of mutant lines
Both laevis and tropicalis will be used

 

The goal is for you to practice the techniques using already known sgRNAs and TALENs and then use your own. Ideally, you will become competent in the techniques and implement them in your own lab. In addition for those who have limited experience in tropicalis, this is a perfect time to get comfortable with them.