Genome Editing

Xenopus Genome Editing Workshop

Marine Biological Laboratory
Woods Hole, MA

Next Workshop: October 18-27, 2018.
Register Here

 

Syllabus (PDF Format)  

 

Workshop Cost:
Event housing, meals and registration, Single room – $1926.60
Event housing, meals and registration, Double room – $1360.60

 

Included in the cost are essential lab consumables, frogs and advance designing and cloning of sgRNA or TALEN pairs specific for your own research. Since the goal of the workshop is to learn the techniques and procedures, it is not imperative that you actually create a line during the 5 days.

 

Many thanks to the National Science Foundation, the National Institutes of Health and the Marine Biological Laboratory for providing funds to reduce the costs associated with this workshop.

Topics covered:
Theoretical and practical aspects of CRISPR/Cas9 and TALENs
Methods for genotyping (DSP, HRMA, T7E1, and others)
Maximizing target site choice and sgRNA design
Applications for the maternal host transfer technique combined with genome editing
Gene targeting and homology-directed repair
Circumventing lethal phenotypes
Xenopus genetics and husbandry issues arising in the creation of mutant lines
Both laevis and tropicalis will be used

 

The goal is for you to practice the techniques using known sgRNAs and TALENs and then use your own. Ideally, you will become competent in the techniques and implement them in your own lab. In addition for those who have limited experience in tropicalis, this is a perfect time to get comfortable with them.