BIE is a three-week course that focuses on comparative anatomy, function, and theoretical background, as well as timely subjects in inner ear biology, medical topics and open questions. These topics will be explored in lecture and laboratory experiments outlined below:
Comparative Anatomy
- zebrafish
- frog
- chicken
- mouse
Microscopy
- SEM
- Confocal
- Spinning disk
- High resolution imaging
Cellular and Developmental Studies
- Organ cultures
- Dissociation and isolation
- Gene delivery
- Paintfill
- Optical clearing methods for immunofluorescence
Electrophysiology
- Intracellular recording from hair cells in cochlear and vestibular epithelium
- Membrane properties in current and voltage-clamp
- Mechanotransduction
- Synaptic signaling
Systems Level Studies
- Auditory brainstem evoked response (ABR)
- Distortion product otoacoustic emissions
- Single unit recording in auditory nerve, brainstem and mid-brain