Teacher Professional Development Workshop on Genome Editing


The goal of this workshop for high school teachers is to provide both theoretical and practical hands-on experience in CRISPR-Cas9 genome editing on Xenopus. The four-day workshop provides a modified version of MBL’s existing advanced training courses offered by the National Xenopus Resource (NXR) and will include both lectures and lab-based experimentation using the amphibian Xenopus laevis as the model. Click here to view faculty bios.

Click to Register

Workshop Dates: June 9-13, 2019

Concepts that will be covered include:

  • introduction to Xenopus as a model for biomedical research
  • basic experimental design concept for the use of CRISPR-Cas9 in the study of development and disease
  • genome navigation for identification of useful targets sites,
  • design of short guide RNA (sgRNA) molecules which provide targeting information to the Cas9 protein
  • synthesis of sgRNA
  • microinjection
  • assessment of mutagenesis efficiency and
  • simple Xenopus embryonic husbandry and care

Although this workshop relies on the use of Xenopus, many of the aspects covered can be easily applied to other systems and organisms. The lectures will provide more advanced understanding of genome editing and advances that have been made in the last five years.

Lab Activities:

  • Injection and embryo sorting
  • Genotyping
  • Transgenic Imaging

Lecture Topics:

  • Introduction to Genome Editing
  • Microinjection and sgRNA synthesis
  • Introduction to Xenopus
  • Navigating the Xenome and sgRNA design
  • Evaluation of CRISPR mutants
  • Recent Advances in Genome Editing

Participant Cost: $2,500

As this new program becomes more established, MBL will identify funding to cover tuition for teachers.

Cost Includes:

  • Housing accommodations
  • Meals
  • Lab supplies and equipment
  • Intensive, hands-on lab and classroom instruction
  • Highly favorable student-teacher ratio fostering up-close access to instructors

Ideal for:

  • Biology teachers
  • AP/IB Science teachers
  • STEM teachers

High School Student Courses

MBL now offers week-long courses for high school students. Currently, students must register for these courses through their schools. Visit our student program webpage for more information.


Alison Crawford, Chief of Staff, Marine Biological Laboratory, acrawford@mbl.edu
Peter Wilson, Deputy Dean of Admissions and Chief of Staff, The University of Chicago, peterwilson@uchicago.edu