Caenorhabditis elegans Module


In this module, students use a variety of complementary experimental techniques to study sensory processing and behavior in the nematode Caenorhabditis elegans. This module continues the theme of using state-of-the art approaches to study and manipulate the function of neurons within the brains of intact, behaving animals. C. elegans is an excellent preparation for this task, with a complete neural wiring diagram, genetic access to each neuron, optogenetic tools for neural stimulation and activity recording by calcium imaging, detailed behavioral analysis, and an extensive library of genetic mutants. Information flow can be traced from sensory stimulus to neural signals to behavioral output, in living and behaving animals.

In the first few days of the modules, all students are trained in pairs to perform a series of complementary experiments in recording and analysis of nematode behavior, neural stimulation and imaging, and C. elegans culture. Behavioral assays are performed using machine vision cameras and MATLAB- or LabView-based quantification systems. Three distinct sensory modalities are typically used for behavioral and neural activity analyses: olfactory, mechanical, and thermal stimulation. Precise chemical stimulation is performed using microfluidic arenas and chambers. Mechanical stimulation is delivered through an Arduino controlled tapper, thermal stimuli are precisely regulated using peltier-based systems. Behavior and neural imaging systems are capable of optogenetic activation or silencing of specific neurons via multicolor LEDs. In the first few days, students are taught how to run many experiments to give them a sense for which types of experiments are best for different project questions. Students are tasked with replicating real, published experiments, to gain confidence with each system. Because these systems are well-established and their use is relatively simple, students can become facile at the core techniques within the first few days. Students continue to build their expertise with the use of MATLAB for designing stimuli, running experiments, and performing analysis. We also use the first few days of the module to familiarize students with the basics of C. elegans neurobiology and genetics, and provide mini ‘boot camps’ on genetic crosses, RNAi, molecular biology, and other relevant topics.

By the fourth day of the first week, the students are sufficiently competent to plan their own projects. The focus of these projects changes from year-to-year as the faculty endeavor to compile a list of ideas based on current research in the field as well as the interests of the students. One consistent theme over the last several years has been imaging neural activity in intact animals during sensory stimulation and behavior using genetically encoded calcium indicators. Students have worked on projects investigating sensory adaptation, synaptic plasticity, and the interplay between sensory and interneuron activity. For instance, one project in compared sensory adaptation between natural sensory stimuli and optogenetic manipulations. Another project investigated the functional importance of a plastic synaptic connection by bypassing this plasticity with a genetically encoded synthetic gap junction to artificially re-wire the C. elegans connectome. In 2018, one project assessed the role of interneurons in behavioral decision-making by tracking activity within interneurons of freely moving animals during directed migration on a thermal gradient. One of the major outcomes of this module is that students come to realize the power of molecular biology and genetics to address questions with precision and clarity. The well-established imaging and behavioral systems, in conjunction with the ability of faculty to bring a wide variety of new worm strains each year, provides a powerful foundation for students to explore discovery-driven projects based on current interest and state-of-the-art genetic tools.


C. elegans Module Faculty and Teaching Assistants

Mark Alkema
University of Massachusetts Medical School

The goal of my research is to understand how the nervous system orchestrates complex behaviors and how evolutionary forces shape behavior. We are addressing this question by studying the escape response of the nematode C. elegans. We have shown that the escape response allows C. elegans increases it chances to escape from predacious fungi that use constricting rings to entrap nematodes. My lab is elucidating how neurons, neurotransmitters and ion channels define neural circuits control independent motor programs, and how these motor programs are linked temporally in the execution of a compound motor sequence. The simplicity and completely defined synaptic connectivity of C. elegans nervous system in combination with powerful genetic methods, optogenetics, calcium imaging and electrophysiology allows us to address how the nervous controls behavior with a cellular and molecular resolution that cannot be readily attained in any other system. Mark has been a faculty member since 2014.


Arantza Barrios
University College London

I did my PhD in Developmental Biology using zebrafish, under the supervision of Steve Wilson at UCL. In 2005 I moved to New York to the laboratory of Scott Emmons at Einstein and later the lab of Maureen Barr at Rutgers to work on neural circuits and behaviour in C. elegans. Since 2012 I have been a group leader at UCL where I continue to use the worm to understand how innate and learned behaviours are encoded in the nervous system.

mei-zhen-profile-photoMei Zhen
University of Toronto

Dr Mei Zhen received her PhD in Biochemistry with Dr Peter Candido at the University of British Columbia. She pursued her postdoctoral training at the University of California, Santa Cruz with Dr Yishi Jin on synapse development. She established her research program at the University of Toronto in 2001. She has been a full professor at the Departments of Molecular Genetics and Physiology since 2008, and a senior scientist at the Lunenfeld-Tanenbaum Research Institute since 2011. Her research interests include neuronal excitability, synapse development, connectomics, and motor functions.

She was a long-term human frontier fellow, a Canada Research Chair in developmental neurobiology, an EJLB Foundation Scholar, and a Harvard Radcliffe fellow.


jeremy-flormanJeremy Florman
UMass Medical

I am interested in how complex behavior is coordinated at the level of the neural circuit. My work has focused on understanding how a neural circuit integrates information and generates an appropriate behavioral output using the escape response in C. elegans as a model of complex behavior. Using detailed behavioral analysis in conjunction with genetic manipulation, laser cell ablation, optogenetics and calcium imaging I have studied the functional mechanisms underlying the escape circuit. I’ve found that a combination of excitatory and inhibitory feed-forward and feed-back connections, as well as peptidergic neuromodulation act in concert to fine tune circuit activity and orchestrate the escape response. I am currently a 5th year graduate student in Mark Alkema’s lab at Umass Medical School. Jeremy joined the C. elegans team in 2017.

Jun Meng
University of Toronto

Jun Meng is a Ph. D student in Mei zhen lab. She previously studied synaptic remodeling using nematode C. elegans as the model organism.  Her current interests lie in investigating the molecular mechanisms for neuronal and circuit excitability in C.elegans by genetics, optogenetics, and live calcium imaging.

Laura Molina-Garcia
University College London

I am interested in how conflicting information is processed by the nervous system during learning to promote a specific behavior. Stimuli are often encountered during events that are both rewarding and aversive simultaneously but our brain can integrate them and generate a behavioural response. How this process occurs at the level of neural circuit activity is not well understood. To shed light into this question I am dissecting a neural circuit for associative learning in C. elegans. I am currently a postdoc in the Barrios lab at UCL where we combine genetic, behavioural and imaging approaches  with the aim of directly link changes in neuronal communication to behavior in freely behaving animals.


Ben Mulcahy
Lunenfeld-Tanenbaum Research Institute

I did my PhD at the University of Southampton, UK, under the tutelage of Professor Vincent O’Connor and Professor Lindy Holden-Dye. Here, I investigated how aging affects synaptic transmission at the C. elegans neuromuscular junction using behavior, pharmacology and electrophysiology.

I am currently a postdoc at the Lunenfeld-Tanenbaum Research Institute, Toronto, in the lab of Professor Mei Zhen, where I continue to explore C. elegans neurobiology. Most of my time is spent using electron microscopy to extract cellular strategies used by the nervous system during developmental remodeling. I also still dabble in electrophysiology and functional imaging of the nervous system (calcium imaging, voltage imaging).