Transgenic Laevis

NXR_0001

Description: (Brainbow H) Default state is ubiquitous whole cell red fluorescence. Following Cre induced recombination cells will have membrane yellow or membrane cyan fluorescence.

Reference: Nature. 2007 Nov;450:56-62. 

NXR_0002

Description: (BrainbowL) Default state is ubiquitous whole cell red fluorescence. Following Cre induced recombination cells will have whole cell yellow or whole cell cyan fluorescence.

Reference: Nature. 2007 Nov;450:56-62.

NXR_0003

Description: (C5) Whole embryo blue fluorescence; yellow fluorescence upon Cre or FLP recombination.

Available as frozen sperm, please allow 8 months for live animals.

Reference: Nucleic Acids Res. 2003 Apr;31:e44. Dev Dyn. 2006 Aug;235:2220-8. 

NXR_0004

Description: Fluorescence detectable in most tissues through development.

Available as frozen sperm, please allow 8 months for live animals.

Reference: Mead Lab

NXR_0006

Description: (CAG-venus(YFP) LineB) pCAGGS driving Venus YFP expression under the control of the CAG promoter and is followed by the rabbit β-globin polyA signal sequence.

Reference: Nat Biotechnol. 2002 Jan;20:87-90. Dev Dyn. 2005 Jun;233:562-9. 

NXR_0007

Description: (Cardiac actin:GFP) 580bp of X. laevis actc1b gene driving GFP expression. Embryonic striated muscle including heart.

Reference: Dev Biol. 2002 May;5:57-70.  Development. 2003 Aug;130(16): 3865-76.

NXR_0008

Description: (CMV-GFP) CMV promoter driving GFP expression, Whole embryo green fluorescence.

Reference:

NXR_0009

Description: Initial ubiquitous green fluorescence switches to red fluorescence following FLP recombinase activation. The constitutive simian cytomegalovirus CMV IE94 promoter/enhancer driving GFP flanked by FRT sites (for FLP site specific recombination). After FLP recombination, CMV drives expression of dsRed.

Previous name: Xla.Tg(CMV:GFP,RFP)^Amaya

Available as frozen sperm, please allow 8 months for live animals.

Reference: Gene. 1996 Nov;180:225-7.   Nucleic Acids Res. 2001 Jun;29:E53-3.  Nucleic Acids Res. 2003 Apr;31:e44.

NXR_0010

Description: (Col2a1-GFP) X. tropicalis collagen 2α1 promoter and first intron driving eGFP, γ-cry driving GFP marker. GFP expression in cartilaginous skeleton.

Reference: Int J Dev Biol. 2010;54(1):141-50. Xenbase.

NXR_0011

Description: (Ef1α-GFP) Eef1a1 gene driving GFP expression, γ-cry driving GFP marker. Widespread  GFP expression apparent from mid-gastrula on.

Available as frozen sperm, please allow 8 months for live animals.

Reference: Gene. 1994 Sep;147:223-6.

NXR_0012

Description: (CMV:memGFP) CMV promoter driving GFP in cell membrane, γ-cry driving mCherry marker. Ubiquitous membrane GFP expression and mCherry in lens.

Reference: Custom transgenic generated by the NXR.

NXR_0013

Description: (Elas-GFP) Rat elastase promoter driving GFP. GFP expression in the pancreas.

Reference: Mech Dev. 1999 Nov;88:221-7. Development. 2009 Jun;136:1791-800. Dev Biol. 2007 Apr;304(2): 786-99.   Curr Biol. 2003 Jan;13(2):105-15. Xenbase.

NXR_0014

Description: (CAG-KikumeGR) CAG promoter driving green-to-red photoconvertible fluorescent protein, Kikume Green-Red (KikGR)

Reference: BMC Development Biology 2009 Sept; 9:49.  Development. 2013;140:2409-2421.

NXR_0016

Description: (HyPer) CMV promoter driving YFP of H2O2 indicator, HyPer. γ-cry driving RFP. Ubiquitous HyPer YFP expression, marker for integration selection; RFP expression in lens.

Reference: EXRC.

NXR_0017

Description: (FLH-GFP) Zebrafish flh promoter driving GFP in the pineal gland and posterior notochord.

Reference:  EXRC. Concha, M., et. al. (2003). Local tissue interactions across the dorsal midline of the forebrain establish CNS laterality. Neuron; 39(3):423-38. Xenbase.

NXR_0018

Description: (FLK1-GFp) 2.5kb X. laevis flk-1 promoter driving GFP. GFP expression in vascular endothelial cells.

Reference: Mead Lab. Dev Dyn. 2007 Oct;236:2808-17.  Xenbase.

NXR_0019

Description: (GATA1-GFP) GATA-1 promoter and first intron driving expression of GFP, sleeping beauty. GFP expression in red blood cells.

Reference: Mead Lab. Xenbase.

NXR_0020

Description: (HGEM-Noggin) Lenses glow green; heat-shock inducible Noggin.

Reference: Dev Cell. 2003 Sep;5:429-39. Mech Dev. 2006 Sep;123:674-88.  Xenbase.

NXR_0021

Description: (HGEM-Xtdkk1) Heat-shock inducible Dkk1 to down-regulate Wnt/B-catenin signaling with a Gamma crystallin GFP selection marker.

Reference: Dev Biol. 2008 Apr 15;316(2):323-35. Xenbase.

NXR_0025

Description: (LURP1-GFP) 5kb of X.laevis slurp1l gene driving eGFP. Embryonic macrophages, definitive myeloid blood cells, restricted neural expression, epidermis.

Reference: Mech Dev. 2002 Sep;117:173-86. Dev Growth Differ. 2006 Oct;48:499-512. Xenbase.

NXR_0026

Description: 8kb of X. laevis myl3 gene driving enhanced GFP. GFP expression in heart mycoardium.

Previous names: MLC1V_GFP, mlc3:eGFP

Reference:  Dev Dyn. 2005 Apr;232(4):1003-12.  Xenbase.

NXR_0027

Description: (MLC2-GFP) 3kb of X. laevis myosin light chain promoter driving eGFP. eGFP expression in ventricular charmber, heart myocardium, and interhyoid facial muscle.

Reference: Development. 2004 Feb;131(3):669-79. Xenbase.

NXR_0030

Description: (Nkx2.5-GFP) 8kb promoter of X. laevis nkx2-5 gene driving GFP expression. Early heart field, mesoderm, pharyngeal, endoderm, spleen.

Reference: Dev Biol. 2000 Nov 1;227(1):65-79. Xenbase.

NXR_0033

Description: (pHS-bCatenin-GFP:Elas-GFP) Generated via REMI (without restriction enzyme) with a cassette containing heatshock promoter driving expression of N-term truncated beta-catenin (stabilized CA form) tagged with GFP. Presumably the cassette also included elas:GFP but that is not described in the paper. The original vector from which pHsβcat∗GFP was derived was named pCH85.

Reference: Developmental Biology, 2008, Sept 15; 316(2): 323-335.

NXR_0034

Description: (SMAD-GFP) 1.5kb of X. laevis Smad3 gene driving eGFP. Dorsal mesoderm post gastrulation, heart endocardium, widespread expression at larval stages.

Reference: Mech Dev. 2011 May-Jun;128:303-15. Xenbase.

NXR_0035

Description: (NBT/Tubb2-GFP) 3481bp  promoter and 5’UTR of tubb2b driving expression pf mapt-fusion. Green fluorescence in neaural tissue.

Reference: Xenbase.

NXR_0036

Description: (xCry-GFP) The crystalline –GFP construct was generated by sub-cloning 2.2 kb a fragment of the crystalline genomic DNA encompassing the promoter region (received from Robert Grainger), upstream of an enhanced version of the GFP3.

Reference: Gene. 1993 Jun;128:189-95.

NXR_0040

Description: (Y2) Whole embryo blue fluorescence; yellow fluorescence upon Cre recombination.

Reference: Nucleic Acids Res. 2003 Apr 15;31:e44.

NXR_0047

Description: (Foxi1-Katushka) 2000bp X. tropicalis foxi I promoter driving expression of Katushka, γ-cry driving Venus marker. Katushka expression in epidermal cells and Venus in lens as integration marker.

Reference: EXRC. Xenbase.

NXR_0049

Description: (Pax3-Katushka) 2916bp X. laevis Pax3 promoter driving expression of Katushka in the neural ectoderm (neural crest).

Reference:  Pax3 promoter from Roberto Mayor (Mauricio Moreno). Xenbase.

NXR_0050

Description: (Brachyury-Katushka) 3856bp X. tropicalis Brachyury promoter driving expression of Katushka, γ-cry driving Venus marker. Katushka expression in muscles post-gastrulation and Venus in lens as integration marker.

Reference: EXRC

NXR_0051

Description: (Xic-GFP) 4.7 kb X. tropicalis  p27 Xic1 promoter driving GFP expression in the neural tube and the eye.

Available as frozen sperm, please allow 8 months for live animals.

Reference: EXRC. Carruthersa, S., et. al. (2003) “Depletion of the cell-cycle inhibitor p27Xic1 impairs neuronal differentiation and increases the number of ElrCþ progenitor cells in Xenopus tropicalis.” Mech of Dev. 120; 607–616. Xenbase.

NXR_0053

Description: (TubulinA1-GFP) Rat tubulin a-1 promoter fused in grame to GFP. GFP expression in the nervous system and later in growing limbs.

Reference: EXRC. Marsh-Armstrong, N., et. al. (1999) “Germ-line transmission of transgenes in Xenopus laevis.” PNAS 96, 14389-14393. Xenbase.

NXR_0056

Description: (Insulin-memGFP) Laevis 5kb insulin promoter driving memGFP expression; gamma crystallin mCherry selection

Reference: Transgenic generated by Donald Brown.

NXR_0059

Description: (Dria-GFP) CMV driving eGFP tagged OMP25 transmembrane segment. GFP expression of mitochondria membranes.

Reference: Dev Growth Differ. October 1, 2012; 54 (8): 767-76.

NXR_0062

Description: (Tubb2-GFP-Aequorin)

Reference: Custom transgenic generated by the NXR for Andrew Miller.

NXR_0063

Description: (CMV-Aequorin-GFP) Aequorin is a Ca²⁺ dependent bioluminescence photoprotein from hydrozoan A. Displays an emission peak at 470nm (blue light).

Reference:Custom transgenic generated by the NXR for Andrew Miller.

NXR_0064

Description: (pbin7LEF-GFP/Wnt Reporter) Construct contains a synthetic Wnt-responsive promoter – consisting of 7 copies of a TCF/LEF1 binding DNA element, a minimal TATA box and a reporter gene encoding destabilized EGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence. GFP expression of dynamic patterns of Wnt/β-catenin signalling activity.

Reference: Proc Natl Acad Sci USA. 2010 Sep 14; 107(37): 16160-16165.

NXR_0067

Description: (XOPNTR) Rod optsin promoter driving E. coli nitroreductase.

Reference: FEBS Lett. 1998 Feb;423:117-21. Invest Ophthalmol Vis Sci. 2011 Jan;52:364-73.

NXR_0069

Description: (SCL-GFP) Mouse SCL +19 enhancer which contains a GATA-ETS-GATA core motif driving GFP. Expression in haematopoietic precursors.

Reference: EMBO J. 2002 Jun 17;21(12):3039-50. Mol Cell Biol. 2010 Dec;30(24):5741-51. Xenbase. EXRC.

NXR_0074

Description: (AlphaTubulin-UTR-RFP) tubulin alpha 1a promoter driving expression of membrane bound red fluorescent protein primarily in the ciliated epidermal cells.

Reference: Custom transgenic generated by the NXR for John Wallingford.

NXR_0092

Description: (CMV-Butterfly VSFP1.2) VSFP is voltage sensitive fluorescence protein. VSFP-Butterfly 1.2 enables optical recordings at high spatial resolution with intrinsic assignment of recorded signals to VSFP-targeted (neural) cell populations.

Reference: Custom transgenic generated by the NXR for Mike Levin.

NXR_0094

Description: (CMV-H2BmRFP) CMV promoter driving RFP in nuclear membrane, γ-cry driving Venus marker. Ubiquitous nuclear RFP expression, and Venus expression in lens.

Reference: Custom transgenic generated by the NXR.

NXR_0095

Description: (CMV-VSFP2.42-SRL) vsFP is a voltage sensitive Fluorescent Protein. vsFP enables optical recordings at high spatial resolution with an intrinsic assignment of recorded signals to vsFP-targeted (neural) cell populations.

Reference: Custom transgenic generated by the NXR for Mike Levin.

NXR_0100

Description: (Centrin-RFP) CMV promoter driving RFP and (cetn4) fused with RFP. RFP expression in the microtubules of cilia/ciliated cells.

Reference: Custom transgenic generated by the NXR for Brian Mitchell.

NXR_0102

Description: (Cdh17inteGFP) Zebrafish(Dre) Li-cadherin (CDH17) driving eGFP. GFP expression in the pronephros/pronephric tubes.

Reference: Custom transgenic generated by the NXR for Rachel Miller. Genes 2018, 9(4).

NXR_0103

Description: (Deup1-GFP) Tubulin promoter driving Deup1 fused to eGFP. eGFP expression of deuterosomes and centrioles specifically in multi-ciliated cells. Marker for visualizing centrioles.

Reference: Custom transgenic generated by the NXR for Brian Mitchell. Xenbase.

NXR_0106

Description: (mPrx1-GFP) GFP labels mesenchymal cells in limb bud and limb blastema.

Reference: EXRC. Dev Biol 304 (2007) 675–686. Xenbase.

NXR_0107

Description: (ElasGFP:Tubb2-GCaMP6s) Neaural expression of the green Ca²⁺ sensor, GCaMP6s, and expression of GFP in the pancreas

Reference: Custom transgenic generated by the NXR.

NXR_0108

Description: (Sox10-GFP) Sox10:GFP-HS4-Isce1 transgene 4.7kb upstream from sox10 transcription factor. GFP expression in the migratory cranial neural crest cells.

Reference: EXRC. Xenbase.

NXR_0109

Description: (EB3-GFP) CMV driving eGFP of human EB3. Ubiquitous expression of eGFP in microtubule plus ends

Reference: EXRC. Takagi et al. 2013 Dev. Growth Differ 55, 422-433.

NXR_0110

Description: (GFP-Golgi) CMV driving GFP of hb4GalT transgene. Ubiquitous expression of eGFP in the golgi appartus.

Reference: EXRC. Takagi et al. 2013 Dev. Growth Differ 55, 422-433.

NXR_0.0111

Description: (HSP Cre1(C5)) Cross of HSP:Cre1 and C5, heat shocked in embryogensis and EYFP positive tadpoles were grown.

Reference: EXRC.

NXR_0113

Description: (RFP-ER) CMV promoter driving RFP in CALR targeting sequence. Ubiquitous expression of RFP in the endoplasmic reticulum.

Reference: EXRC. Takagi et al. 2013 Dev. Growth Differ 55, 422-433. Xenbase.

NXR_0114

Description: (LMO2-Katushka) 4775bp X. tropicalis LMO2 promtoer driving Katushka, γ-cry driving VenusGFP marker. Blood marked with red fluroescent protein Katushka, and Venus YFP variant expressed in the lens.

Reference: EXRC. Xenbase.

NXR_0115

Description: (RFP-Mem) CMV promoter driving RFP in cell membrane. Ubiquitous membrane RFP expression.

Reference:  EXRC. Takagi et al. 2013 Dev. Growth Differ 55, 422-433.

NXR_0118

Description: (mpeg-EGFP,crygamCherry) Zebrafish mpeg promoter driving GFP, γ-cry driving mCherry marker. GFP expression in the macrophages and mCherry in the lens.

Reference: EXRC. Ellett, F. et. al. (2011) “Mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish”. Blood; 117(4):e49-56.

NXR_0119

Description: (CMV:EosFP)

Reference: Custom transgenic line generated by the NXR for Michael Levin.

NXR_0121

Description: (Hsp:Cre1) Cre recombinase drive by HSP70

Reference: EXRC. Roose,M., et. al. (2009) “Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis.” Trans Res. 18, 595-605.

NXR_0122

Description: (eGFP-ATP1a3) Xenopus rhodopsin promoter driving expression of eGFP tagged neuronal α3 isoform of the Na⁺/K⁺-ATPase in the rod photoreceptors.

Referenece: Traffic. 2015 Dec;16(12):1239-53. Xenbase.

NXR_0123

Description: (Ubi-Gal4:UAS-venusTau:UAS-mCherryMoe)

Ubiquitous red fluorescence marking F-actin and green fluorescence marking microtubules. Cyan fluorescence marking the eye lens. Line was generated by digestion of three plasmids with Asp718 and then co-injection via REMI. First plasmid: 1.5 kb of human ubiquitin C promoter driving expression of Gal4-VP16 and gamma crystalin promoter driving expression of CFP. Second plasmid: UAS driving expression of mCherry tagged moesin. Third plasmid: UAS driving expression of Venus tagged microtubule associated protein tau.

Reference: EXRC

NXR_0125

Description: (Elas:GFP,Tubb2bChIEF) Neaural expression of blue light photoinducible channelrhodopsin, ChIEF, and expression of GFP in the pancreas.

Reference: Custom transgenic generated by the NXR.

NXR_0126

Description: (HSP70:dnYAP-2A-GFP,gCry:tdTomato) 330 bp of promoter of the X. laevis hsp70 gene driving the expression dominant negative form of Yap (dnYap) tagged with GFP via 2A peptrides of 2.2 kb gamma crystalline promoter driving tdTomato.

Reference: EXRC.

NXR_0127

Description: Detects hydrogen peroxide in vivo. Ubiquitous expression of YFP in tissues with reactive oxygen species (ROS).

Reference: EXRC.

NXR_0128

Description: (HSP-XFDvenus; cryRFP) HSP70 promoter driving dominant negative FGFR with crystallin-RFP.

Reference: EXRC.

NXR_0129

Description: (Ubic dsRed-Mito) 1.5kb of UbiC promoter driving dsRed and COX8, γ-cry driving Venus marker. Ubquitous dsRed expression in mitochondria, and Venus in lens.

Reference: EXRC.

NXR_0130

Description: (Mintbody/CMV-H3K9ac-EGFP) CMV promoter driving eGFP of h3k9ac transgene. Ubiquitous GFP histone expression.

Reference: Ueno.  Genes Cells. 2016 21:358–369. Sci Rep. 2013; 3:2436.

NXR_0132

Description: (Pax8-GFP) Pax8 driving GFP. GFP expression of kidney and head.

Reference: Nat Commun. 2012 May 22;3:848. Xenbase.

NXR_0134

Description: (NIBB6/Scat3) CAG-seCFP-Venus (an enhanced yellow fluorescent protein with efficient maturation property), containing the fluorescence indicator molecule SCAT3 and the rabbit β-globin polyA signal sequence, under control of the CAG promoter. SCAT3 is a hybrid protein of seCFP-Venus linked with the caspase-3 recognition sequence (DEVD) in pCAGGS.

Reference: Ueno Lab. Kominami, K. et al., 2006 Genes Cells 11:701-711. Takagi C. et al. 2013 Dev. Growth Differ 55, 422-433.

NXR_0135

Description: (Elas-rtTA25-M2/tet(O)-NICD,cryGFP) Tetracycline inducible activated Notch expression in the exocrine pancreas.

Reference: Dev. Biology. 2011 351:2, 311-317.

Xla.Tg(CMV:hist2h2be-GFP;CMV:mRFP)^Flc

NXR_0139

Description: (CMV:H2B-GFP;CMV:mRFP) Cross of CMV:mRFP parent created CMV IE94 promoter in pCS2+ backbone, RFP inserted at BstBI and linearized with NotI; and CMV:H2B-GFP parent. Ubiquitous GFP expression of nuclear membrane and ubiquitous RFP expression of cell membrane.

Reference: Conlon Lab. H2B Xenbase.