Transgenic Tropicalis

 

Transgenic Line Image
Xtr.Et(CAGGS-SB10;cryga:RFP)622EMead

NXR_1002

Description: (622EHoppers) SB10 transposase expression from CAGGS promoter/enhancer, γ-cry driving RFP. 622E donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events.

Reference: Mead

622e_sb111

Source: NXR

Xtr.Et(CAGGS-SB10;cryga:RFP)7M8FMead

NXR_1003

Description: (7M8FHoppers) SB10 transposase expression from CAGGS promoter/enhancer, γ-cry driving RFP. 7M and 8F donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events.

Reference: Mead

Xtr.Et(CAGGS-SB10;cryga:RFP)7MMead

NXR_1004

Description: (7MHoppers) SB10 transposase expression from CAGGS promoter/enhancer, γ-cry driving RFP. 7M donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events.

Reference: Mead

Source: Mead

Source: Mead

Xtr.Et(CAGGS-SB10;cryga:RFP)8FMead

NXR_1005

Description: (8FHoppers) SB10 transposase expression from CAGGS promoter/enhancer, γ-cry driving RFP. 8F donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events.

Reference: Mead

Xtr.Tg(CMV:memGFP)NXR

NXR_1007

Description: (CMVmemGFP) CMV driving ubiquitous expression of membrane GFP

Reference: Custom line generated by the NXR.

cmvmemgfp

Source: NXR

Source: NXR

Source: NXR

Xtr.Tg(eef1a1:GFP)Krieg

NXR_1008

Description: (Ef1a-GFP) Eef1a1 driving GFP. Widespread GFP expression apparent from mid-gastrula on.

Reference: Gene. 1994 Sep 30;147(2):223-6. Xenbase.

Xtr.Et(gata1:GFP)mysMead

NXR_1012

Description: (mTol2G1GFP-M4msy) GATA-1 promoter driving GFP, mister stripey integration into the apelin receptor gene. GFP expression in red blood cells and venous vasculature.

Reference: Mead Lab; Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Source: Mead

Source: Mead

Xtr.Et(krt8:GAL4-VP16,UAS:GFP)Mead

NXR_1014

Description: (mTol2k8GAL4 VP16 UASGFP) Keratin 8 minimal promoter driving expression of GAL4VP16 fusion with UAS GFP reporter gene. Made using the miniTol2 transposon.

Reference: Mead Lab; Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11

Xtr.Tg(otx2:GFP)Kellr

NXR_1020

Description: (Otx2-GFP) Otx2 driving GFP. GFP expression of forebrain and midbrain

Reference:  Development. 1995 Apr; 121(4): 993-1004. Development. 1995 Mar; 121(3): 707-20. Xenbase.

Source: NXR

Source: NXR

otx2gfp_1

Source: NXR

Xtr.Tg(pax6:GFP;cryga:RFP;actc1:RFP)Papal

NXR_1021

Description: (Pax6-GFP:xcryFRP:CardiacActin-RFP) Coinjection of pax6 driving GFP, γ-cry driving RFP, and actc1 driving RFP. GFP expression in eyes, brain, and spinal cord. RFP expression in lenses and muscle.

Reference:  Dev Biol. 2001 Oct 1; 238(1): 168-84. Dev. Dyn. 2002 Dec; 225(4): 552-35.

pax6tripletrans_1

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Xtr.Et(CAGGS-SB10;cryga:RFP; CAGGS:GFP)622EMead

NXR_1025

Description: (pT2BGFP-622E) Widespread GFP fluorescence: outcross of the ‘hopper’ frogs will result in remobilization of the substate transposon and re-integration elsewhere in the genome resulting in expression pattern and/or intensity of GFP expression.

Reference: Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.

Source: Mead

Source: Mead

Xtr.Et(CAGGS-SB10,cryga:RFP; CAGGS:GFP)6MMead

NXR_1026

Description: (pT2BGFP-6M) Widespread GFP fluorescence.

Reference: Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.

Source: Mead

Source: Mead

Xtr.Et(CAGGS-SB10,cryga:RFP; CAGGS:GFP)8FMead

NXR_1028

Description: (pT2BGFP-8F) LDouble transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events.

Reference: Mead Lab; Dev Dyn. 2009 Jul; 238(7); 1727-43.  Mob DNA. 2011 Nov 24; 2: 15.

Xtr.Et(CAGGS-SB10;cryga:RFP)Mead

NXR_1034

Description: (SB10enzyme) SB10 transposase expression from CAGGS promoter/enhancer, γ-cry driving RFP.

Reference: Mead

Xtr.Et(eef1a1:GFP)Hbr,Cen,Fol,BruMead

NXR_1037

Description: (Tol2X1G-12MHbrCenFolBru) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP.

Reference: Mead

Xtr.Et(eef1a1:GFP)folMead

NXR_1038

Description: (Tol2X1G-12Mfol) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Fol integration into 5′ UTR of MTHFD1L gene.

Reference:  Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Source: Mead

Source: Meadtol

Xtr.Et(eef1a1:GFP)cenMead

NXR_1040

Description: (Tol2X1G-Mcen) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Cen expressed in forebrain.

Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Source: Mead

Source: Mead

Xtr.Et(eef1a1:GFP)grbMead

NXR_1042

Description:  (Tol2X1G-12Mgrb) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP.  Garibaldi (grb) integration into 3′ UTR of smad7 gene.

Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Source: Mead

Source: Mead

Xtr.Et(eef1a1:GFP)hbrMead

NXR_1043

Description: (Tol2X1G-12Mhbr) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Expression in cartilage and cardiac outflow tract.

Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Source: Mead

Source: Mead

Source: NXR

Source: NXR

Xtr.Et(eef1a1:GFP)johMead

NXR_1044

Description: (Tol2X1G-12Mjoh) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Joh expressed in kidney, integration near HNF1B gene.

Reference:  Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

tol2x1gjoh
Xtr.Et(eef1a1:GFP)psvbiMead

NXR_1046

Description: (Tol2X1G-16Fpsvbi) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Medial somite and ventral blood island expression. Integration likely to be in repeart regiong of MLL2 gene.

Reference: Mead Xenbase.

Source: Mead

Source: Mead

Xtr.Et(eef1a1:GFP)16FMead

NXR_1047

Description: (Tol2X1G-16Fubiq) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. 16F fubiq

Reference: Mead Lab;  Genesis. 2006 Sep;44(9):438-45. BMC Dev Biol. 2010 Jan 22;10:11. Xenbase.

Xtr.Tg(cryga:GFP)Grngr

NXR_1049

Description: (xCrys-GFP) GFP expression in the lens.

Reference: Grainger. Xenbase.

Xtr.Tg(CMV:Aequorin,GFP)NXR

NXR_1070

Description: (CMV-Aequorin-GFP)

Reference: Custom line generated by NXR for Andrew Miller.

Xtr.Tg(tubb2b:GFP)Amaya

NXR_1092

Description: (NBT/Tubb2-GFP) N-tubulin promoter driving GFP. GFP expression in neural tissue.

Reference: EXRC. Xenbase.

Source: EXRC

Source: EXRC

Xtr.Tg(vim:Venus;cryga:GFP)Amaya

NXR_1093

Description: (Vimentin-GFP) Vimentin promoter driving Venus GFP, γ-cry driving GFP marker. GFP expression labels the deep cells of the neurectoderm and neural progenitors. GFP expression in lens.

Reference: EXRC. Xenbase.

Source: NXR

Source: NXR

xcryrfpvimentingfp_1

Source: NXR

Xtr.Tg(WntREs:dEGFP)Vlemx

NXR_1094

Description: (pbin7LEF-GFP/Wnt Reporter) GFP expression reveals dynamic patterns of Wnt/beta-catenin signaling activity. The inserted transgene construct contains a synthetic Wnt-responsive promoter consisting of 7 copies of a TCF/LEF1 binding DNA element, a minimal TATA box and a reporter gene encoding destabilized eGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence.

Reference: EXRC.

Source: EXRC

Source: EXRC

pbin7lefgfp

Source: NXR

Xtr.Tg(pax6:PR-Gal4-VP16;cryga:eCFP)Zimml

NXR_1098

Description: (Pax6galPRBicolorBlue) Pax6 promoter driving RU486-inducible progesterone receptor/VP16/Gal4 transactivator, in transgene marked by g-cry ECFP cassette. ECFP in lens of eye; inducible Gal4 drives expression of UAS-transgenes in the presence of RU486 in the eye and dorsal CNS. Labels lens of eye; other functions depend on separate effector UAS transgenes

Reference: EXRC. Xenbase.

Xtr.Tg(tubb2b:Hsa.ALPP;cryga:dsRed)Amaya

NXR_1099

Description: (NBT-PLAPxcryRFP) Neural expression of human placental alkaline phosphatase (ALPP) driven by N-beta-tubulin (tubb2b) upstream region. dsRed expression in eye from gamma crystallin (cryga) promoter used as marker.

Reference: EXRC. XenbaseBMC Devlp. Bio. 2007 May; 7:59.

Xtr.Tg(Hsa.UBC.:lyn-memVenus)Amaya

NXR_1106

Description: (UbiC-membraneVenus) Human Ubiquitin C (Hsa.UBC) promoter driving expression of a lyn kinase membrane domain fused to venus GFP. Ubiquitous Venus expression of cell membrane.

Reference: EXRC. Xenbase.

ubiqmemvenus

Source: NXR

Xtr.Tg(pax6:GFP)Amaya

NXR_1107

Description: (Pax6-GFP) 3.6kb PAX6 promoter driving GFP. GFP expression in anterior and posterior neural plate plate.

Reference: EXRC. Xenbase.

Source: NXR

Source: NXR

Source: NXR

Source: NXR

Xtr.Tg(UbiC Flox RFPGFP)

NXR_1108

Description: Ubiquitous expression, floxed RFP GFP reporter. Ubiquitously expressed RFP; upon cre-medmiated recombination expresses GFP and loses RFP.

Reference: Transgenic generated by Bettina Ryll. EXRC

Xtr.Tg(Ntub GalPR BicB)

NXR_1109

Description: (Ntub GalPR BicB) Ntub Galpr Bic- N-tubulin promoter driving Gal4Pr (RU482-inducible Gal4-progesterone receptor VP16 fusion) in post-mitotic neurons. Cry-CFP marker cassette (linked)

Reference: EXRC.

Xtr.Tg(Hsa.UBC:Gal4-VP16;cryga:CFP)Amaya

NXR_1110

Description: (UbiqGal4,cryRFP) Human Ubiquitin C (UBC) promoter driving Gal4-VP16 fusion construct, with crystallin CFP marker.

Reference: EXRC. Xenbase.

Xtr.Tg(Dre.mxn1:GFP)Dorey

NXR_1111

Description: (zHB9-GFP) Zebrafish HB9 promoter driving GFP. GFP expression in motorneurons and kidney.

Reference: EXRC. Xenbase.

Source: NXR

Source: NXR

Xtr.Tg(UAS:msn-mCherry;cryga:RFP)Amaya

NXR_1117

Description: (UASmCherry-Moesin) UUAS driving mCherry-Moesin, γ-cry driving RFP marker. Labels F-actin.

Reference: Xenbase. EXRC.