Genome Editing Workshop

nxr-genome-editing-2019Next Workshop: TBD

Syllabus (PDF Format)
Topics covered:

  • Theoretical and practical aspects of CRISPR/Cas9 and TALENs
  • Methods for genotyping (DSP, HRMA, T7E1, and others)
  • Maximizing target site choice and sgRNA design
  • Applications for the maternal host transfer technique combined with genome editing
  • Gene targeting and homology-directed repair
  • Circumventing lethal phenotypes
  • Xenopus genetics and husbandry issues arising in the creation of mutant lines
  • Both laevis and tropicalis will be used

The goal is for you to practice the techniques using known sgRNAs and TALENs and then use your own. Ideally, you will become competent in the techniques and implement them in your own lab. In addition for those who have limited experience in tropicalis, this is a perfect time to get comfortable with them.