(These materials are from previous years – 2015 materials coming soon!)
ARTICLES and ADDITIONAL MATERIALS BY TOPIC:
General Zebrafish Development: Kimmel et al 1995 Neural Anatomy and Images (from Wilson and Houart labs) Early Development: Solnica-Krezel et al 1996 Hafter et al 1996 Solnica-Krezel and Sepich 2012 Langdon and Mullins 2011 Ho and Kane 1990 Genome Editing (TALENs and CRISPRs): Bedell et al 2012 Ma et al., 2013 Neff et al., 2013 Jao_et_al_2013
Imaging and Germ-cell migration: Kardash et al 2010 Raz and Reichman 2006 Tarbashevich and Raz 2010 Labeling and expression of inducible transgenes: Hutson and Chien_2002 Culverwell_et al 2003 Placintaetal2009_LaserHeater Shen and Ozacar etal 2013 Karlstrom_LocalHeater_DetailProtocol Imaging and Mounting Samples: Kaufmann et al 2012_(multilayer mounting) PIT TALKS: Zfish_intro_Lecture_LSK Gastrulation_LSK Comparative_Neuroanatomy Ekker MBL talk 2013 (genome editing technologies) and Flow chart for TALEN assembly & screening (make your own TALENs) Mullins_Pit talk_morpholinos Introduction to Microscopy (Andres Collazo) Karlstrom_PitTalk_HS and DyeLabel Transplants and Chimeras Pit Talk 2013 (Cecilia Moens and Michael Granato) Late Transplants Pit Talk 2013 (Corinne Houart) Imaging Cellular and Subcellular Structures: Debbie Yelon (Pit Talk), Dave Raible (Pit Talk), Jon Clarke (Pit Talk)
Zebrafish Chemical Screens Motor and Visual Behaviors Pit Talks:Michael Granato and Jim Fadool ROUND TABLE DISCUSSIONS: Mutagenesis Tools: Mutagenesis Tools Mullins Ekker Roundtable_Protein Trap
Zebrafish husbandry, building a facility, breeding and raising fry:
ZIRC
Mutagenesis Tools:
Transgenesis_Roundtable Slides 2013
Responsible Conduct of Research: Image Manipulation:
Please read the following papers for Thursday Aug 15th.
-NSF Intro to Responsible Research Conduct
-Rossner & Yamada 2006
-Seeing is believing_JCB 2006
Aquatic Systems, Fish Facility Management:
Techniplast
Pentair
Imaging, Atlas and TG reporter resources:
Transgenic resources, imaging and expression atlases: Corinne Houart and Jon Clarke
VIDEOS:
Please click on the links below for course-related video.
Walker, Charline and Walsh, Greg S and Moens, Cecilia (2009) Making gynogenetic diploid zebrafish by early pressure. Journal of visualized experiments : JoVE (28). 1396. ISSN 1940-087X
http://authors.fhcrc.org/324
Draper, Bruce W and Moens, Cecilia B (2009) A high-throughput method for zebrafish sperm cryopreservation and in vitro fertilization. Journal of visualized experiments : JoVE (29). 1395. ISSN 1940-087X
http://authors.fhcrc.org/323
Kemp, Hilary A and Carmany-Rampey, Amanda and Moens, Cecilia (2009) Generating chimeric zebrafish embryos by transplantation. Journal of visualized experiments : JoVE (29). 1394. ISSN 1940-087X
http://authors.fhcrc.org/322