Analytical and Quantitative Light Microscopy

Course Description

A comprehensive and intensive course in light microscopy for researchers in biology, medicine, and materials science. AQLM provides a systematic and in-depth examination of the theory of image formation, microscopy techniques, and quantitative image analysis. AQLM students are graduate students, post-docs, core directors and staff, faculty, and industry scientists. Enrollment is limited to 32 students.

Laboratory exercises, demonstrations, and discussions include:

1. geometrical and physical optics of microscope image formation;
2. label-free microscopy techniques including quantitative phase imaging and polarization microscopy;
3. fluorescence microscopy and quantification of fluorescence;
4. fluorescent proteins and fluorescence biosensors;
5. digital sensors and digital image processing;
6. live cell imaging;
7. confocal microscopy;
8. superresolution microscopy;
9. light-sheet microscopy; and
10. open-source microscopy

Lectures are followed by small-group laboratory sessions using cutting-edge microscopes from our industry partners guided by academic and industry faculty.