Optical Microscopy & Imaging in the Biomedical Sciences

Course Information

Course Date: August 20 – August 30, 2018

Deadline: May 15, 2018 | Apply here

2017 Course Schedule (PDF)

Directors: Joerg Bewersdorf, Yale University; and Alison North, The Rockefeller University

Course Description

This course is designed primarily for research scientists, postdoctoral trainees, and advanced graduate students in animal, plant, medical, and material sciences. Non-biologists seeking a comprehensive introduction to microscopy and digital imaging will benefit greatly from the course. Some prior theoretical or practical understanding of the basic principles of optics and microscopy is necessary. This 10 day course is limited to 26 students. It consists of correlated lectures, laboratory exercises, demonstrations, and discussions that will enable the participant to obtain and interpret microscope images of high quality, to perform quantitative optical measurements, and to produce high quality digital video, and digital records for documentation and analysis.

Topics to be covered include: (a) fundamental principles of microscope design, image formation, resolution, contrast; (b) bright field, dark field, phase contrast, polarized light, differential interference contrast, interference reflection, and fluorescence microscopy; (c) cameras, signal to noise ratio, digital image recording, processing and analysis, multispectral imaging; (d) advanced fluorescence– fluorescent probes, TIRF, FRET, FLIM, FRAP, polarization of fluorescence, fluorescence correlation spectroscopy; (e) digital image restoration/deconvolution, and 3-D imaging principles, confocal scanning microscopy, multiphoton excitation fluorescence microscopy, light-sheet microscopy; application of the optical methods to live cells will be emphasized, although other specimens also will be discussed; (f) super-resolution techniques including localization microscopy, stimulated emission depletion microscopy (STED), structured illumination microscopy. Particular emphasis will be placed on ‘picking the right tool for the job’.

Students will have direct hands-on experience with state-of-the-art microscopes, digital cameras, recorders, and image processing equipment provided by major optical, electronics, and software companies. Instruction will be provided by experienced staff from universities and industry. Students are encouraged to bring their own biological and material specimens, and to discuss individual research problems with the faculty.

2017 Course Faculty & Lecturers

Bewersdorf, Joerg
Clemenceau, Philippe, Imagine Optic
Day, Richard, Indiana University
DePasquale, Joe
Elliott, Amicia, NIH
Fullerton, Stephanie, Hamamatsu
Goodwin, Paul, General Electric
Hard, Robert, SUNY Buffalo
Lanni, Fred, Carnegie Mellon University
Lavis, Luke, HHMI Janelia Research Campus
Leung, Jacqueline, Indiana University
McCall, Andrew, SUNY Buffalo
McIlvain, Jim, MBL/Zeiss
McNally, Jim, Helmoltz Institute Berlin
Murray, John, Indiana University
North, Alison, Rockefeller University
Prabhat, Prashant
Preza, Chrysanthe, University of Memphis
Shroff, Hari, SUNY Buffalo
Sigurdson, Wade, SUNY Buffalo
Winfree, Seth, Indiana University