The 2017 Zebrafish Development and Genetics Course will be held August 4-18, 2017.
Over the past 20 years, the zebrafish has emerged as a powerful model system for the study of vertebrate development and disease. This intensive two-week course for advanced graduate students, postdoctoral fellows, and independent investigators will focus on the development and genetics of zebrafish. It will cover standard and novel technologies geared towards their application in zebrafish. Mornings and afternoons will be devoted mainly to laboratory exercises and the evenings to lectures and discussion. Limited to 22 students. See also a printable poster to help us advertise the course.
Lectures and labs will introduce students to early development of the zebrafish and to methods for manipulating and studying gene function, including genetic and small molecule screening, mapping and cloning; mRNA overexpression and functional knockdown approaches; genome editing techniques; cell fate mapping; in vivo biophysics; mounting and imaging of antibody and in situ samples for publication ready documentation. In addition, students will be introduced to techniques for labeling and visualizing individual cells, including photoconversion, cell transplantation/chimera analysis, and live cell imaging. They will also learn how to perform behavioral tests on live animals. Several informal ‘roundtable’ discussions will cover the design and execution of mutant screens/reverse genetics, transgenesis, zebrafish breeding and husbandry, as well as data management and figure construction.
Each laboratory exercise will be under the supervision of a senior faculty member who will be assisted by one or two junior faculty members. Senior faculty will also give research lectures about their work and lead discussions about topics and concepts covered in the course.
2017 Faculty & Lecturers
- Sharon Amacher (Ohio State University)
- Jon Clarke (Kings College London)
- Andres Collazo (Caltech)
- Elena Dreosti (University College London)
- Jim Fadool (Florida State University)
- Steven Farber (Carnegie Institute)
- Antonio Giraldez (Yale University)
- Michael Granato (University of Pennsylvania)
- Corinne Houart (Kings College London)
- Anna Huttenlocher (University of Wisconsin, Madison)
- Cecilia Moens (Fred Hutchinson Cancer Research Center)
- Mary Mullins (University of Pennsylvania)
- Kenneth Poss (Duke University)
- Dave Raible (University of Washington)
- Erez Raz (University of Münster)
- Alex Schier (Harvard University)
- Lila Solnica-Krezel (Washington University)
- Steve Wilson (University College London)
- Debbie Yelon (University of California San Diego)
2017 Course Coordinators (click to contact)
2017 Zebrafish Husbandry Coordinators
April Freeman and Caitlin Inglis