The 2017 Zebrafish Development and Genetics Course will be held Aug 4-19.
Corinne Houart, King’s College, UK
Sharon Amacher, Ohio State University, USA
Erez Raz, University of Münster, Germany
Lectures and labs will introduce students to early development of the zebrafish and to methods for manipulating and studying gene function, including genetic and small molecule screening, mapping and cloning; mRNA overexpression and functional knockdown approaches; genome editing techniques; cell fate mapping; in vivo biophysics; mounting and imaging of antibody and in situ samples for publication ready documentation. In addition, students will be introduced to techniques for labeling and visualizing individual cells, including photoconversion, cell transplantation/chimera analysis, and live cell imaging. They will also learn how to perform behavioral tests on live animals. Several informal ‘roundtable’ discussions will cover the design and execution of mutant screens/reverse genetics, transgenesis, zebrafish breeding and husbandry, as well as data management and figure construction.
Each laboratory exercise will be under the supervision of a senior faculty member who will be assisted by one or two junior faculty members. Senior faculty will also give research lectures about their work and lead discussions about topics and concepts covered in the course.
This year, to mark its 20th anniversary, the course will conclude with a special symposium, highlighting work of many course alumni.
2017 Faculty & Lecturers
- Sharon Amacher (Ohio State University)
- Jon Clarke (Kings College London)
- Andres Collazo (Caltech)
- Elena Dreosti (University College London)
- Jim Fadool (Florida State University)
- Steven Farber (Carnegie Institute)
- Antonio Giraldez (Yale University)
- Marcos Gonzalez-Gaitan (University of Geneva)
- Michael Granato (University of Pennsylvania)
- Corinne Houart (Kings College London)
- Anna Huttenlocher (University of Wisconsin, Madison)
- Cecilia Moens (Fred Hutchinson Cancer Research Center)
- Mary Mullins (University of Pennsylvania)
- Kenneth Poss (Duke University)
- Dave Raible (University of Washington)
- Erez Raz (University of Münster)
- Alex Schier (Harvard University)
- Lila Solnica-Krezel (Washington University)
- Steve Wilson (University College London)
- Debbie Yelon (University of California San Diego)
2017 Course Coordinators (click to contact)
2017 Zebrafish Husbandry Coordinators
April Freeman and Caitlin Inglis