LSFM Program
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Monday, May 8, 2023:
Speck Auditorium, Rowe Building, MBL
14:00 – 15:00
Registration
15:00 – 15:15
Welcoming Remarks
Gary Laevsky, Princeton University
Abhishek Kumar, Marine Biological Laboratory
15:15 – 16:00
“Optical Sectioning and basic fluorescence microscopy”
Michelle Itano, University of North Carolina Chapel Hill
16:00 – 16:45
“Anatomy of a lightsheet microscope”
Kate McDole, MRC Laboratory of Molecular Biology
16:45 – 17:00
Coffee Break
17:00 – 17:45
“Choosing cameras for purpose, Cost vs function vs speed.”
Simon Watkins, University of Pittsburgh
17:45 – 18:30
“Practical aspects of objective lens design”
Steven Ross, Nikon USA (Virtual)
18:30 – 20:30
Dinner and Poster
20:30 – 21:15
“Considerations for lightsheet image processing and analysis”
Holly Gibbs, Texas A&M University
Tuesday, May 9, 2023:
Speck Auditorium, Rowe Building, MBL
08:45 – 09:00
Introduction
Gary Laevsky, Princeton University
09:00 – 09:45
“Light-sheet fluorescence microscopy across spatial and temporal scales”
Reto Fiolka, U T Southwestern
09:45 – 10:30
“Timekeeping in endosomal trafficking”
Senthil Arumugam, Monash University
10:30 – 11:00
Coffee Break
11:00 – 11:45
“Imaging seizures in zebrafish with volumetric light sheet imaging and adaptive optics”
Peter Kner, University of Georgia
11:45 – 12:30
“Light sheet for the masses!”
Emmanuel Reynaud, University College of Dublin
12:30 – 13:30
Lunch
13:30 – 14:15
“A modular approach to tissue clearing”
Douglas Richardson, Harvard Medical School
14:15 – 15:15
“Early Career Session”
“Decoding how biology uses physics to sculpt an inner organ”
Noah Mitchell, University of California, Santa Barbara
“Developing new tools for exploring cephalopod embryogenesis”
Caroline Albertin, Marine Biological Laboratory
“Understanding neurodevelopment and disease across scales in zebrafish”
Liz Haynes, Morgridge Institute for Research
15:15 – 15:30
Coffee Break
15:30 – 17:30
Vendor Talks
17:30 – 20:30
Reception Dinner, Meigs Room
20:30 – 22:00
Data Handling and Image Analysis (Main Lab, Loeb Building)
Wednesday, May 10, 2023:
Loeb Laboratory, Main Lab 2nd Floor, MBL
08:45 – 09:00
Introduction
Gary Laevsky, Princeton University
09:00 – 09:45
“Practical considerations for quantitative light sheet fluorescence microscopy”
Chad Hobson, Janelia Research Campus
10:00 – 12:00
Laboratory 1
12:00 – 13:30
Lunch
13:30 – 15:30
Laboratory 2
15:30 – 16:00
Coffee Break
16:00 – 18:00
Laboratory 3
18:30 – 21:30
“What can you do with really fast light-sheet microscopy?”
Keynote Lecture, Meigs Room
Elizabeth Hillman, Columbia University
21:30 – 22:30
Free Time on Microscopes
Thursday, May 11, 2023:
Loeb Laboratory, Main Lab 2nd Floor, MBL
08:45 – 09:00
Introduction
Gary Laevsky, Princeton University
09:00 – 11:00
Laboratory 4
11:00 – 13:00
Laboratory 5
13:00 – 14:30
Lunch (Box Lunch)
14:30 – 16:30
Laboratory 6
16:30 – 17:00
Coffee Break
17:00 – 18:30
Data Handling and Image Analysis
“Tutorial in Image Processing For Light Sheet Microscopy”
Anthony Mautino, Marine Biological Laboratory
“Multi-view Image Fusion in FIJI”
Carsten Wolff, Marine Biological Laboratory
18:30 – 21:30
Dinner and Workshop Fun with Kate and Emmanuel
21:30 – 22:30
Free Time on Microscopes
Friday, May 12, 2023:
Loeb Laboratory, Main Lab 2nd Floor, MBL
08:45 – 09:00
Introduction
Gary Laevsky, Princeton University
09:00 – 09:45
“Seeing is believing: using lightsheet microscopy to gain cell biological insights into zebrafish eye and brain development with undergraduates”
Kara Cerveny, Reed College
10:00 – 12:00
Laboratory 7
12:00 – 13:00
Lunch
13:00 – 15:00
Laboratory 8
15:00 – 15:30
Coffee Break
15:30 – 17:30
Laboratory 9
17:30 – 18:00
Discussion
18:00 – 20:00
Dinner
20:00 – 22:00
Free Time on Microscopes
Saturday, May 13, 2023:
Loeb Laboratory, Main Lab 2nd Floor, MBL
08:45 – 09:00
Introduction
Gary Laevsky, Princeton University
09:00 – 09:45
“The path to the lightsheet: sample preparation considerations”
Rebecca Adikes, Siena College
10:00 – 12:00
Laboratory 10
12:00 – 13:00
Lunch
13:00 – 15:00
Laboratory 11
15:00 – 15:30
Coffee Break
15:30 – 17:30
Data Handling and Image Analysis
17:30 – 18:00
Discussion
18:00 – 21:00
Dinner, Student Lightening Talks, Happy Hour