Overview
Comparative Developmental Biology is an intensive two-week laboratory course for graduate students in year two of their studies or beyond and post-docs, who seek a broad training in experimental approaches to developmental questions across diverse study organisms.
Course Date: October 6-20, 2024
Application due date: June 21, 2024
Course description:
This intensive comparative developmental biology course is designed for graduate students in their second or later year of Ph.D studies and post-docs. The two weeklong course will provide exposure to a combination of well-established and emerging developmental systems. Students will develop advanced experimental embryology skills—many of which are transferable across organisms—in the handling and cellular/genetic manipulation of embryos, including microinjection, lineage tracing, microdissection, cell transplantation, in situ hybridization, CRISPR/Cas mutagenesis, and 3D in vivo imaging. Students will develop an enhanced appreciation of the advantages each species offers, will be trained to think more comparatively (in a phylogenetic context), and will gain an appreciation of how best to select the appropriate species to address a specific question. They will be exposed to classic, recent, and developing methodologies and techniques and will learn about exciting ongoing research using these approaches. Developing and completing a short independent or team-based research project will enhance skills in hypothesis generation and experimental design.
Financial Information
Cost: $4000.00 including room and board
Course Objectives
During this two-week course, students will gain hands-on familiarity with a range of traditional and emerging model systems in developmental biology. They will gain experience in microscopy, image acquisition and analysis, as well as in embryological, pharmacological, and molecular genetic approaches to address key questions in development and regeneration. Students will understand the specific advantages of each model system, gain insights into the types of problem that are currently being researched in each model, and gain experience with key cross-cutting techniques such as CRISPR/Cas mutagenesis, lineage tracing, and in situ hybridization. Students will also enhance their hypothesis building and experimental design and analysis skills through weeklong independent or small-team projects.
The following models are expected to be available in 2024: Zebrafish, Drosophila, Parhyale hawaiensis, Butterfly, Nematostella, Squid, and Skate.
Course Directors:
Nipam Patel
MBL Director
My lab works to uncover both developmental and evolutionary mechanisms in a variety of animals that help us understand the generation of biodiversity, with a recent focus on the crustacean, Parhyale, and several species of butterflies. We use Parhyale to understand the role of Hox genes and other developmental genes in building and evolving body plans and also investigate the mechanisms that allows this species to regenerate its germline. With butterflies, we are working to discover how single scale cells create the impressive nanostructures they use for structural coloration, without which they would be greatly restricted in the pallet of colors available to them.
Vicky Prince
Professor, Organismal Biology & Anatomy, University of Chicago
At the Prince lab we take a cellular, molecular, and comparative approach to the study of developmental processes. Our research program uses rapidly developing, transparent—and very beautiful—embryos of the zebrafish, Danio rerio. Zebrafish embryos are ideal for high resolution live imaging approaches, and these techniques are helping us to understand dynamic cellular processes that build complex structures during embryonic development—especially of the nervous system and cranial structures. Our imaging experiments are complemented by the use of increasingly powerful molecular genetic and transgenic tools, such as CRISPR/Cas technology, which allow us to interrogate the molecular basis of developmental processes. Please take a look around our pages to learn more about our ongoing studies.
Course Faculty:
Caroline Albertin
Assistant Scientist, Bell Center
The Albertin lab studies how novelty arises over the course of evolution and embryogenesis using cephalopods as models. We employ diverse approaches, including comparative genomics, gene expression studies, and live imaging, with a focus on understanding (i) molecular patterning of cephalopod body plans, (ii) cellular and molecular events in cephalopod neurogenesis, (iii) the evolution of the cephalopod fate map and developmental program, and (iv) developing new molecular tools and approaches to enable research in cephalopods.
Karen Echeverri
Associate Scientist, Bell Center
The ability to regenerate complex tissue has fascinated scientists for a long time. The Echeverri lab is interested in the cell and molecular mechanisms driving regeneration. We study regeneration in axolotls; salamanders well known for their ability to functionally regenerate multiple body parts, including limbs, tail, heart, eyes and jaw and in addition can repair lesions in the brain and heal all wounds without forming scar tissue. My group uses cell and molecular tools combined with in vivo imaging and genomics approaches to decipher the key circuitry and cellular mechanisms that are essential to promote functional regeneration.
Andrew Gillis
Associate Scientist, Bay Paul Center
The Gillis Lab studies embryonic development of the skeletal, sensory, and neuroendocrine systems of vertebrate animals. Our work uses a comparative approach and a diverse array of emerging and established model organisms to reconstruct the origin and early evolution of the vertebrate body plan. Current lines of research include 1) development, growth and repair of the vertebrate skeleton, 2) serial homology and the origin of anatomical novelty, and 3) the evolution of neuroendocrine cell type diversity.