OMIBS Schedule
This is a draft schedule and will likely be updated.
|
Time |
Location |
Event |
Lecturer |
|---|---|---|---|
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DAY 1 - Monday, August 14, 2023 |
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5:00 PM-6:00 PM |
Swope |
Walk around Woods Hole (optional; meet in front of Swope) |
Angie Di Domenico, Malea Murphy, Abbi Sathapppan |
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6:00 PM-7:00 PM |
Speck |
Welcome and orientation |
Alison North, Joerg Bewersdorf |
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7:00 PM-8:00 PM |
Swope Terrace |
Pizza party |
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8:00 PM-9:00 PM |
Speck |
Lecture: Grand overview of the course |
Alison North |
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DAY 2 - Tuesday, August 15, 2023 |
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8:15 AM-9:30 AM |
Speck |
Lecture: Foundations of microscopy 1 - geometrical optics |
Joerg Bewersdorf |
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9:30 AM-10:00 AM |
Break |
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10:00 AM-11:00 AM |
Speck |
Lecture: Foundations of microscopy 2 - lenses and optical systems |
Joerg Bewersdorf |
|
11:00 AM-12:00 PM |
Loeb 2nd floor |
Lab: Basic Sample preparation |
Malea Murphy, Abbi Sathapppan, Michelle Itano, Alison North |
|
12:00 PM-1:00 PM |
Swope |
Lunch |
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|
1:00 PM-3:15 PM |
Loeb 2nd floor |
Lab: Build basic microscopes |
Mark Lessard, Jyot Antani |
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3:15 PM-3:30 PM |
Loeb 2nd floor |
Break |
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|
3:30 PM-6:30 PM |
Loeb 2nd floor |
Lab: Practice with commercial microscope stands |
Jacqueline Leung + TAs |
|
6:30 PM-7:30 PM |
Swope |
Dinner (meeting for vendors: last 15 min of dinner) |
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|
7:30 PM-9:00 PM |
Speck |
Lecture: Foundations of microscopy 3 - wave optics |
Joerg Bewersdorf |
|
9:00 PM-10:00 PM |
Loeb 2nd floor |
Demonstration: Abbe diffraction apparatus |
Chris Bjornsson |
|
DAY 3 - Wednesday, August 16, 2023 |
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8:15 AM-9:30 AM |
Speck |
Lecture: Transmitted light 1 |
Sharon King |
|
9:30 AM-9:45 AM |
Break |
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|
9:45 AM-11:15 AM |
Speck |
Lecture: Cameras |
Stephanie Fullerton |
|
11:15 AM-12:15 PM |
Loeb 2nd floor |
Lab: Phase contrast microscopy |
Sharon King, Jacqueline Leung, TAs |
|
12:15 PM-1:15 PM |
Swope |
Lunch |
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|
1:15 PM-3:15 PM |
Loeb 2nd floor |
Lab: ImageJ basics |
Andrew McCall |
|
3:15 PM-4:15 PM |
Loeb 2nd floor |
Lab: Use of cameras |
Andrew McCall, Jyot Antani |
|
4:15 PM-5:30 PM |
Speck |
Lecture: Transmitted light 2 |
Sharon King |
|
5:30 PM-6:30 PM |
Loeb 2nd floor |
Lab: DIC and polarization (part 1) |
Sharon King, Jacqueline Leung, TAs |
|
6:30 PM-7:30 PM |
Swope |
Dinner (meeting for vendors: last 15 min of dinner) |
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7:30 PM - 10:30 PM |
Loeb 2nd floor | Lab: DIC and polarization (part 2) | Sharon King, Jacqueline Leung, TAs |
|
DAY 4 - Thursday, August 17, 2023 |
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8:15 AM-9:00 AM |
Speck |
Discussion of lab exercises |
Sharon King |
|
9:00 AM-9:45 AM |
Speck |
Lecture: Photophysics of fluorescence and dye chemistry |
Luke Lavis |
|
9:45 AM-10:45 AM |
Speck |
Lecture: Introduction to the fluorescence microscope |
Alison North |
|
10:45 AM-11:00 AM |
Break |
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|
11:00 AM-12:30 PM |
Loeb 2nd floor |
Lab: Fluorescence microscopy (part 1) |
Alison North, Michelle Itano, Jacqueline Leung |
|
12:30 PM-1:30 PM |
Swope |
Lunch |
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|
1:30 PM-2:00 PM |
Speck |
Lecture: Colocalization |
Alison North |
|
2:00 PM-2:30 PM |
Speck |
Lecture: Instrumentation for live-cell imaging |
Michelle Itano |
|
2:30 PM-3:00 PM |
Speck |
Lecture: Fluorescence 2 - Chemical tags for functional imaging |
Luke Lavis |
|
3:00 PM-3:20 PM |
Speck |
Tech bites 1 |
Alison North, commercial faculty |
|
3:20 PM-3:30 PM |
Break |
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|
3:30 PM-4:00 PM |
Loeb 2nd floor |
Lab: Cleaning optical filters |
Mark Lessard |
|
4:00 PM-6:00 PM |
Loeb 2nd floor |
Lab: Fluorescence microscopy (part 2) |
Alison North, Michelle Itano, Jacqueline Leung |
|
6:00 PM-7:00 PM |
Swope |
Dinner (meeting for vendors: last 15 min of dinner) |
|
|
7:00 PM-9:30 PM |
Loeb 2nd floor |
Lab: Build widefield fluorescence microscope |
Mark Lessard, Jyot Antani |
|
9:30 PM-11:00 PM |
Loeb 2nd floor |
Commercial faculty appreciation event |
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|
DAY 5 - Friday, August 18, 2023 |
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8:15 AM-8:30 AM |
Speck |
Discussion of lab exercises |
Alison North, Joerg Bewersdorf |
|
8:30 AM-9:30 AM |
Speck |
Lecture: Fluorescent proteins |
Alison Tebo |
|
9:30 AM-10:00 AM |
Break |
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|
10:00 AM-11:00 AM |
Speck |
Lecture: Imaging dynamics (incl. FRET, FLIM, FCS) |
Mathew Tantama |
|
11:00 AM-12:00 PM |
Speck |
Lecture: Biosensors |
Alison Tebo |
|
12:00 PM-1:00 PM |
Swope |
Lunch |
|
|
1:00 PM-3:15 PM |
Loeb 2nd floor |
Lab: FRET & FLIM |
Alison Tebo, Mathew Tantama, Jacqueline Leung, TAs |
|
3:15 PM-4:00 PM |
Discussion of FRET & FLIM lab exercises |
Alison Tebo, Jacqueline Leung, TAs |
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|
4:00 PM-5:00 PM |
Speck |
Lecture: Sample preparation techniques |
Angie Di Domenico |
|
5:00 PM-5:15 PM |
Break |
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|
5:15 PM-6:15 PM |
Speck(remote) |
Lecture: Principles of digital imaging 1 |
Patrick La Riviere |
|
6:15 PM-7:15 PM |
Swope |
Dinner (meeting for vendors: last 15 min of dinner) |
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|
7:15 PM-8:15 PM |
Speck(remote) |
Lecture: Principles of digital imaging 2 |
Patrick La Riviere |
|
8:15 PM - 10:15 PM |
Speck (remote) |
Lab: Digital imaging |
Patrick La Riviere, Matthew Parent |
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DAY 6 - Saturday, August 19, 2023 |
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8:15 AM-9:00 AM |
Speck |
Discussion of lab exercises |
Patrick La Riviere, Joerg Bewersdorf |
|
9:00 AM-9:45 AM |
Speck(remote) |
Lecture: Evaluating optical performance using PSFs |
Lynne Turnbull |
|
9:45 AM-10:00 AM |
Break |
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|
10:00 AM-12:00 PM |
Loeb 2nd floor |
Lab: Resolution measurements |
Joerg Bewersdorf |
|
12:00 PM-12:30 PM |
Loeb courtyard |
Group Photo |
Mark Lessard |
|
12:30 PM-1:00 PM |
Lunch |
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|
1:00 PM-5:00 PM |
Afternoon off |
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|
5:00 PM-6:00 PM |
Speck |
Lecture: Deconvolution |
Sid Shaw |
|
6:00 PM-7:00 PM |
Swope |
Dinner (meeting for vendors: last 15 min of dinner) |
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|
7:00 PM-8:30 PM |
Speck |
Lecture: Confocal microscopy |
Sid Shaw |
|
8:30 PM-8:45 PM |
Break |
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|
8:45 PM-9:45 PM |
Speck |
Discussion of lab exercises |
Sid Shaw |
|
DAY 7 - Sunday, August 20, 2023 |
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8:15 AM-9:15 AM |
Speck |
Lecture: Multiphoton microscopy |
Cristina Rodriguez |
|
9:15 AM-12:15 PM |
Loeb 2nd floor |
Lab: Deconvolution, confocal, multiphoton (part 1) |
Sid Shaw, Doug Richardson, Cristina Rodriguez, Jacqueline Leung, Angie Di Domenico |
|
12:15 PM-1:15 PM |
Swope |
Lunch |
|
|
1:15 PM-4:15 PM |
Loeb 2nd floor |
Lab: Deconvolution, confocal, multiphoton (part 2) |
Sid Shaw, Doug Richardson, Cristina Rodriguez, Jacqueline Leung, Angie Di Domenico |
|
4:15 PM-4:45 PM |
Break |
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|
4:45 PM-5:15 PM |
Speck |
Lecture: ImageJ tutorial on quantitative analysis |
Sid Shaw |
|
5:15 PM-6:45 PM |
Speck |
Lab: Quantification & analysis (part 1) |
Sid Shaw |
|
6:45 PM-7:45 PM |
Swope |
Dinner (meeting for vendors: last 15 min of dinner) |
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|
7:45 PM-8:45 PM |
Speck |
Lab: Quantification & analysis (part 2) |
Sid Shaw |
|
8:45 PM-10:00 PM |
Speck |
Discussion of Lab Exercises |
Sid Shaw |
|
DAY 8 - Monday, August 21, 2023 |
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8:30 AM-9:30 AM |
Speck |
Lecture: Light sheet microscopy |
Wes Legant |
|
9:30 AM-10:30 AM |
Speck |
Lecture: Tissue clearing and Expansion Microscopy |
Doug Richardson |
|
10:30 AM-11:00 AM |
Break |
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|
11:00 AM-12:00 PM |
Speck |
Lecture: Adaptive optics |
Cristina Rodriguez |
|
12:00 PM-1:00 PM |
Swope |
Lunch |
|
|
1:00 PM-2:00 PM |
Speck |
Lecture: Super-resolution 1 - SIM methods |
Alison North, Joerg Bewersdorf |
|
2:00 PM-5:00 PM |
Loeb 2nd floor |
Lab: Light-sheet microscopes, SIM, iSIM (part 1) |
Alison North, Mark Lessard, Abhishek Kumar, Angie Di Domenico |
|
5:10 PM-6:00 PM |
Speck |
Lecture: Super-resolution 2 - pan Expansion Microscopy |
Joerg Bewersdorf |
|
6:00 PM-7:00 PM |
Swope |
Lecture: Super-resolution 3 - STED |
Joerg Bewersdorf |
|
6:00 PM-7:00 PM |
Swope |
Dinner (meeting for vendors: last 15 min of dinner) |
|
|
7:00 PM-9:00 PM |
Loeb 2nd floor |
Lab: Light-sheet microscopes, SIM, iSIM (part 2) |
Alison North, Mark Lessard, Abhishek Kumar, Angie Di Domenico |
|
9:00 PM-10:30 PM |
Speck |
Tech bites 2 |
Alison North, commercial faculty |
|
DAY 9 - Tuesday, August 22, 2023 |
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8:30 AM-9:30 AM |
Speck |
Lecture: Super-resolution 4 - Single-molecule localization techniques |
Joerg Bewersdorf |
|
9:30 AM-10:30 AM |
Speck |
Lecture: Spatial Omics |
Jacqueline Leung |
|
10:30 AM-12:00 PM |
Loeb 2nd floor |
Lab: STED & single-molecule localization |
Mark Lessard, Angie Di Domenico |
|
12:00 PM-1:00 PM |
Swope |
Lunch |
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|
1:00 PM-4:00 PM |
Loeb 2nd floor |
Lab: Rotations on specialized instruments/demos |
Jacqueline Leung |
|
4:00 PM-5:00 PM |
Speck |
Lecture: Pitfalls and artifacts in super-resolution |
Alison North, Joerg Bewersdorf, Jacqueline Leung |
|
5:00 PM-6:00 PM |
Speck |
Career Panel |
Alison North, Joerg Bewersdorf |
|
6:00 PM-7:00 PM |
Swope |
Dinner (meeting for vendors: last 15 min of dinner) |
|
|
7:00 PM-10:00 PM |
Loeb 2nd floor |
Lab: Time on instruments / demos (part 1) |
Jacqueline Leung, Mark Lessard |
|
DAY 10 - Wednesday, August 23, 2023 |
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|
8:30 AM-12:00 PM |
Speck |
Lab: Time on instruments / demos (part 2) |
Jacqueline Leung, Mark Lessard |
|
12:00 PM-1:00 PM |
Swope |
Lunch |
|
|
1:00 PM-2:00 PM |
Speck |
Discussion of super-resolution labs and critique of images |
Alison North, Joerg Bewersdorf |
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2:00 PM-2:45 PM |
Speck |
Lecture: Ethics and good practices in microscopy |
Michelle Itano |
|
2:45 PM-3:45 PM |
Speck |
Lecture: Imaging live specimens without killing them |
Binyam Mogessie |
|
3:45 PM-4:45 PM |
Discussion: choosing the right microscope for an application |
Alison North, Joerg Bewersdorf |
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4:45 PM-5:00 PM |
Break |
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|
5:00 PM-6:00 PM |
Speck |
Horizons Lecture: "TBD" |
Emma Lundberg |
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6:00 PM-6:30 PM |
Break |
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6:30 PM-9:00 PM |
Swope Terrace |
Banquet |
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DAY 11 - Thursday, August 24, 2023 |
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Morning |
Check-out after Breakfast |
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