2024 OMIBS Schedule

DAY 1 - Tuesday, August 13, 2024

5:00 PM-6:00 PM

Swope

Walk around Woods Hole (optional; meet in front of Swope)

Course Staff

6:00 PM-7:00 PM

Speck

Welcome and orientation

Alison North, Joerg Bewersdorf

7:00 PM-8:00 PM

Swope Terrace

Pizza Party

8:00 PM-9:00 PM

Speck

Lecture: Grand overview of the course

Alison North

DAY 2 - Wednesday, August 14, 2024

8:15 AM-9:30 AM

Speck

Lecture: Foundations of microscopy 1 - geometrical optics

Joerg Bewersdorf

9:30 AM-10:00 AM

Break

10:00 AM-11:00 AM

Speck

Lecture: Foundations of microscopy 2 - lenses and optical systems

Joerg Bewersdorf

11:00 AM-12:00 PM

Loeb 2nd floor

Lab: Basic Sample preparation

Course Staff

12:00 PM-1:00 PM

Swope

Lunch

1:00 PM-3:15 PM

Loeb 2nd floor

Lab: Build basic microscopes

Course Staff

3:15 PM-3:30 PM

Break

3:30 PM-6:30 PM

Loeb 2nd floor

Lab: Practice with commercial microscope stands

Course Staff

6:30 PM-7:30 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:30 PM-9:00 PM

Speck

Lecture: Foundations of microscopy 3 - wave optics

Joerg Bewersdorf

9:00 PM-10:00 PM

Loeb 2nd floor

Demonstration: Abbe diffraction apparatus

Chris Bjornsson

DAY 3 - Thursday, August 15, 2024

8:15 AM-9:30 AM

Speck

Lecture: Transmitted light 1

Sharon King

9:30 AM-9:45 AM

Break

9:45 AM-11:15 AM

Speck

Lecture: Cameras

Stephanie Fullerton

11:15 AM-12:15 PM

Loeb 2nd floor

Lab: Phase contrast microscopy

Course Staff

12:15 PM-1:15 PM

Swope

Lunch

1:15 PM-3:15 PM

Loeb 2nd floor

Lab: ImageJ basics

Andrew McCall

3:15 PM-4:15 PM

Loeb 2nd floor

Lab: Use of cameras

Andrew McCall and Course Staff

4:15 PM-5:30 PM

Speck

Lecture: Transmitted light 2

Sharon King

5:30 PM-6:30 PM

Loeb 2nd floor

Lab: DIC and polarization (part 1)

Sharon King and Course Staff

6:30 PM-7:30 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:30 PM - 10:30 PM

Loeb 2nd floor

Lab: DIC and polarization (part 2)

Sharon King and Course Staff

DAY 4 - Friday, August 16, 2024

8:15 AM-9:00 AM

Speck

Discussion of lab exercises

Sharon King

9:00 AM-9:45 AM

Speck

Lecture: Photophysics of fluorescence and dye chemistry

Luke Lavis

9:45 AM-10:45 AM

Speck

Lecture: Introduction to the fluorescence microscope

Alison North

10:45 AM-11:00 AM

Break

11:00 AM-12:30 PM

Loeb 2nd floor

Lab: Fluorescence microscopy (part 1)

Course Staff

12:30 PM-1:30 PM

Swope

Lunch

1:30 PM-2:00 PM

Speck

Lecture: Chemical tags for functional imaging

Luke Lavis

2:00 PM-3:00 PM

Speck

Lecture: Sample preparation techniques

Alison North

3:00 PM-3:30 PM

Break

3:30 PM-4:00 PM

Loeb 2nd Floor

Lab: Cleaning optical filters

Mark Lessard

4:00 PM-6:00 PM

Loeb 2nd Floor

Lab: Fluorescence microscopy (part 2)

Course Staff

6:00 PM-7:00 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:00 PM-9:30 PM

Loeb 2nd floor

Lab: Build widefield fluorescence microscope

Course Staff

9:30 PM-11:00 PM

Loeb 2nd floor

Commercial faculty appreciation event

DAY 5 - Saturday, August 17, 2024

8:15 AM-8:30 AM

Speck

Discussion of lab exercises

Alison North, Joerg Bewersdorf

8:30 AM-9:30 AM

Speck

Lecture: Fluorescent proteins

Alison Tebo

9:30 AM-10:00 AM

Break

10:00 AM-11:00 AM

Speck

Lecture: Imaging dynamics (incl. FRET, FLIM, FCS)

Mathew Tantama

11:00 AM-12:00 PM

Speck

Lecture: Biosensors

Alison Tebo

12:00 PM-1:00 PM

Swope

Lunch

1:00 PM-3:15 PM

Loeb 2nd floor

Lab: FRET & FLIM

Alison Tebo, Mathew Tantama, Course Staff

3:15 PM-4:00 PM

Discussion of FRET & FLIM lab exercises

Alison Tebo, Mathew Tantama, Course Staff

4:00 PM-5:00 PM

Speck

Lecture: Tissue Clearing

Doug Richardson

5:00 PM-5:15 PM

Break

5:15 PM-6:00 PM

Speck

Lecture: Principles of digital imaging 1

Patrick La Riviere

6:00 PM-7:00 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:00 PM-8:00 PM

Speck

Lecture: Principles of digital imaging 2

Patrick La Riviere

8:00 PM - 9:30 PM

Speck

Lab: Digital imaging

Patrick La Riviere and Course Staff

DAY 6 - Sunday, August 18, 2024
FALMOUTH ROAD RACE - EXPECT ROAD CLOSURES!

8:15 AM-9:00 AM

Speck

Discussion of lab exercises

Patrick La Riviere, Joerg Bewersdorf

9:00 AM-9:45 AM

Speck

Lecture: Evaluating optical performance using PSFs

Lynne Turnbull

9:45 AM-10:00 AM

Break

10:00 AM-12:00 PM

Loeb 2nd floor

Lab: Resolution measurements

Joerg Bewersdorf

12:00 PM-12:30 PM

Loeb courtyard

Group Photo (followed by meeting for vendors)

Mark Lessard

12:30 PM-1:00 PM

Lunch

1:00 PM-5:00 PM

Afternoon off

5:00 PM-6:00 PM

Speck

Lecture: Deconvolution

Sid Shaw

6:00 PM-7:00 PM

Swope

Dinner (NO vendor meeting)

7:00 PM-8:30 PM

Speck

Lecture: Confocal microscopy

Sid Shaw

8:30 PM-8:45 PM

Break

8:45 PM-9:45 PM

Speck

Discussion of lab exercises

Sid Shaw

DAY 7 - Monday, August 19, 2024

8:15 AM-9:15 AM

Speck

Lecture: Multiphoton microscopy

Cristina Rodriguez

9:15 AM-12:15 PM

Loeb 2nd floor

Lab: Deconvolution, confocal, multiphoton (part 1)

Sid Shaw, Doug Richardson, Cristina Rodriguez and Course Staff

12:15 PM-1:15 PM

Swope

Lunch

1:15 PM-4:15 PM

Loeb 2nd floor

Lab: Deconvolution, confocal, multiphoton (part 2)

Sid Shaw, Doug Richardson, Cristina Rodriguez and Course Staff

4:15 PM-4:45 PM

Break

4:45 PM-5:15 PM

Speck

Lecture: ImageJ tutorial on quantitative analysis

Sid Shaw

5:15 PM-6:15 PM

Speck

Lab: Quantification & analysis (part 1)

Sid Shaw

6:15 PM-7:15 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:15 PM-8:45 PM

Speck

Lab: Quantification & analysis (part 2)

Sid Shaw

8:45 PM-10:00 PM

Speck

Discussion of Lab Exercises

Sid Shaw

DAY 8 - Tuesday, August 20, 2024

8:30 AM-9:00 AM

Speck

Lecture: Instrumentation for live-cell imaging

Michelle Itano

9:00 AM-10:00 AM

Speck

Lecture: Imaging live specimens without killing them

Binyam Mogessie

10:00 AM-10:15 AM

Break

10:15-11:15 AM

Speck

Lecture: Light sheet microscopy

Wes Legant

11:15-11:30 AM

Break

11:30 AM-12:30 PM

Speck

Lecture: Adaptive optics

Cristina Rodriguez

12:30 PM-1:30 PM

Swope

Lunch

1:30-2:30 PM

Speck

Lecture: Super-resolution 1 - SIM methods

Alison North, Joerg Bewersdorf

2:30-5:30 PM

Loeb 2nd Floor

Lab: Light-sheet microscopes, SIM, iSIM (part 1)

Course Staff

5:30-6:00 PM

Speck

Lecture: Super-resolution 2 - Expansion Microscopy

Joerg Bewersdorf

6:00 PM-7:00 PM

Swope

Dinner (meeting for vendors: last 15 min of dinner)

7:00 PM-9:00 PM

Loeb 2nd Floor

Lab: Light-sheet microscopes, SIM, iSIM (part 2)

Course Staff

9:00-10:30 PM

Speck

Tech Bites

DAY 9 - Wednesday, August 21, 2024

8:30 AM-9:30 AM

Speck

Lecture: Spatial Omics

Jacqueline Leung

9:30 AM-10:15 AM

Speck

Lecture: Super-resolution 3 - STED

Joerg Bewersdorf

10:15 AM-10:30 AM

Break

10:30 AM-11:30 AM

Speck

Lecture: Super-resolution 4 - Single-molecule localization techniques

Joerg Bewersdorf

11:30 AM-12:15 PM

Loeb 2nd floor

Lab: STED & single-molecule localization (part 1)

Course Staff

12:15 PM-1:15 PM

Swope

Lunch

1:15 PM-2:00 PM

Loeb 2nd Floor

Lab: STED & single-molecule localization (part 2)

Course Staff

2:00 PM-2:15 PM

Swope

Break

2:15 PM - 3:00 PM

Speck

Lecture: Colocalization

Alison North

3:00 PM - 6:00 PM

Lb 2nd floor

Lab: Rotations on specialized instruments/demos

Course Staff

6:00 - 7:00 PM

Swope

Dinner (NO vendor meeting)

7:00-10:00 PM

Loeb 2nd Floor

Lab: Time on instruments/demos (part 1)

DAY 10 - Thursday, August 22, 2024

8:30 AM-12:00 PM

Speck

Lab: Time on instruments / demos (part 2)

Course Staff

12:00 PM-1:00 PM

Swope

Lunch

1:00 PM-2:00 PM

Speck

Discussion of super-resolution

Alison North, Joerg Bewersdorf

2:00 PM-2:45 PM

Speck

Lecture: Ethics and good practices in microscopy

Michelle Itano

2:45 PM-3:45 PM

Speck

Career Panel

Alison North, Joerg Bewersdorf

3:45 PM-4:45 PM

Final Discussion

Alison North, Joerg Bewersdorf

4:45 PM-5:00 PM

Break

5:00 PM-6:00 PM

Speck

Horizons Lecture

Florian Jug

6:00 PM-6:30 PM

Break

6:30 PM-9:00 PM

Swope Terrace

Banquet

DAY 11 - Friday, August 23, 2024

Morning

Check-out after Breakfast