July 29th, Wednesday

Course Directors Arrival for course prep with MBL ADMIN

TA Arrival

TAs begin unpacking the lab and setting up

TAs begin any cell and fungal cultures needed for experiments

July 30th, Thursday

TAs Lab Setup Continues

TAs continue cell and fungal culture prep

TAs Exp1 Microscopy any culture Prep needed

TAs Exp 8 Cryptococcus Macrophage Cultures

July 31st, Friday – **STUDENTS ARRIVE, COURSE BEGINS**

TAs continue cell culture prep

TAs Exp 1 Microscopy of fungal mutants/organelles – Set up fungal cultures, ~3 PM

TAs Exp 2 YNB culture for Cryptococcusgenetics.

5 -7 pm: Opening reception/dinner – Swope Lower Terrace Tent

7 pm: MBL Welcome - Bay Reading ROOM

7:45 pm: Jack Edwards and Aaron Mitchell KeyNote Address: Richard Bennett – Bay Reading ROOM

Reception to Follow- Quad picnic area

August 1st, Saturday

8:30 - 9: MOMY Course Intro – MBL Admin–Loeb 374 Fishbowl

9 -10: Research Seminar: Jamie Konopka - Loeb 374 Fishbowl

10:15-11:15: Research Seminar: Aaron Mitchell - Loeb 374 Fishbowl

11:15:

1:00-2:00: Nuts/Bolts: Exp 1 Microscopy:Jamie Konopka -

2:00:

Fungal Genome Resources – Robb Cramer –Loeb 360 - Lab

Exp 1: Microscopy of Fungal Organelles - INTRODUCTION SESSION – USE 4 ANIMAL GROUPS and cultures from day before.

TAs Exp 2 start Crypto cx for CRISPR

TAs Exp 3: Start Aspergillus culture Aspergillus transformation

7- 9:30: Faculty, TA, student intros + tips and tricks for success at MOMY and in Science – Bay Reading Room

August 2nd, Sunday

8:00: Exp. 3: Aspergillus genetics: Start Culture for A. fumigatusprotoplast generation

9:00 – 10: Research Seminar, Tamara Doering - Loeb 374 Fishbowl

10:15-11:15: Nuts/Bolts Lecture: Exp 2 Crypto genetics/animal models- Tamara Doering

1:00: Nuts/Bolts Aspergillus transformation-Robb Cramer

2:00:

Exp 1: Microscopy of fungal mutants/organelles – SELECT STRAINS 4 GROUPS

Exp 2: Crypto CRISPR transformation: Day 2 electroporate and plate

Exp 3: Aspergillus transformation – protoplasting and transformation

TAs Exp 4: Lobster model of aspergillosis – A nidulans cultures

7:00: Faculty Mentoring:Manuscripts and Publishing – Bay Reading Room (Faculty Co-leaders to be recruited)

August 3rd, Monday

8 AM: Exp 3 Aspergillus transformation Day 1 – hygromycin overlay

9 - 10: Research Seminar: Robb Cramer - Loeb 374 Fishbowl

10:15: Research SeminarAnita Sil Loeb 374 Fishbowl

1-7p:

ACF Walk Through with Students – meet at lab, walk over together

Exp 1: Microscopy of fungal mutants/organelles

Exp 2: Crypto CRISPR transformation – check plates

Exp 3: Aspergillus transformation Day 1 – check plates in PM

TAs Exp 4: Lobster model of aspergillosis – day -1 – Immune suppress, prep inoculum

TAs Exp 5: Zebrafish – Streak out Candidastrains

7:00 Group Discussion – Artificial Intelligence (AI) Usage in Mycology – Bay Reading Room

August 4th, Tuesday

8:15:

9 -10: Research Seminar:Sarah Beattie - Loeb 374 Fishbowl

10:15-11:15: Nuts/Blots: Exp 4 Lobster AspergillosisSarah Beattie: Loeb 374 Fishbowl

1-7:

Exp 1: Microscopy of fungal mutants/organelles

Exp 2: Crypto CRISPR transformation – Day 4 check plates, pick colonies and patch

Exp 3: Aspergillus transformation Day 2 – Pick colonies to new plates

Exp 4: Lobster model of aspergillosis – Day 0 – Inoculate with intranasal instillation

-Come to ARC in groups starting at 1 PM, 45 minutes/group

Exp 5: Zebrafish – Prepare Candida Culture Streaks for colonies, set up spawning tank

7:00: Rigor, Reproducibility, Ethics in Science – Faculty small groups

9:00: Exp 5 – Zebrafish – Prepare Candida Overnight Cultures for Injection

August 5th, Wednesday

9 - 10: Research Seminar, Rob Wheeler – Loeb 374 Fish Bowl

10:15: Nuts/Bolts – Exp 5 Zebrafish Fungal Infections –Rob Wheeler– Loeb 374

1-7: Exp 1 – Microscopy of fungal mutants/organelles

Exp 2 Crypto CRISPR transformation Day 5 replica plate, start cultures for capsule induction/imaging

Exp 3 Aspergillus transformation Day 3 – Collect conidia/colony PCR – start phenotyping (if ready)

Exp 4: Lobster model of aspergillosis – Day 1 – Caspofungin injections, monitor

Exp 5: Zebrafish: (1) Inoculate with Candida(2) Collect Embryos and change media (3) set up spawning tank

5:00: TA Dinner withWheeler/Nett

7:30 pm: Faculty mentoring – Careers in Academia - Bay Reading Room (Faculty Co-leaders to be recruited)

August 6th, Thursday

9:00 -10:00: Research Seminar: David Rogers - Loeb 374 Fishbowl

10:15: Research Seminar: Scott Moye-Rowley- Loeb 374 Fishbowl

1:00:

Exp 1 – Microscopy of fungal mutants/organelles

Exp 2 Crypto transformation Day 6 – check replica plates, imaging, start capsule induction–Epifluorescence Zeiss Observer Scope 1-5 p.m.

Exp 3: Aspergillus transformation Day 4 – Collect conidia/colony PCR – start phenotyping (if not ready prior day)

Exp 4: Lobster model of aspergillosis – day 2 – Caspofungin injections, monitor

Exp 5: Zebrafish – Collect Embryos and infection ½ students inject, ½ students image–

Confocal noon - 6

TAs Exp 8 Cryptococcus Macrophage Cultures

7:00: Seminar Dona Love – NIH and NIH Grants – Bay Reading Room

7:45: Faculty Panel: Grants (opportunities, writing, reviews) –Bay Reading Room

August 7th, Friday

9-10: Research Seminar: Kevin Fuller - Loeb 374 Fishbowl

10:15: Research Seminar: Xiarong LinLoeb 374 Fishbowl

11:15:

1-7:

Exp 2: Crypto CRISPR transformation Day 7 Image induced cells for

Capsule – Epifluorescence Zeiss Observer Scope 1-5 p.m.

Exp 3: Aspergillus transformation Day 5 –Monitor Phenotypes

Exp 4: Lobster model of aspergillosis – Day 3 – Caspofungin Injections, monitor

Exp 5: Zebrafish – Collect Embryos and infection ½ students inject, ½ students image –

Confocal noon - 6

TAs Exp 8 Cryptococcus Macrophage Cultures

7:00 PM: Exp 4: Lobster model of aspergillosis – Day 3.5 - harvest, flash freeze, lyophilize

8:00 PM: MBL Friday Evening Lecture: George O’Toole: The Microbiome from Guts to Lungs (Doors open at 7:30 Clapp Auditorium)

August 8th, Saturday

10AM-3PM: Science Stroll Woods Hole (optional)

9-10: Research Seminar: Jeniel Nett- Loeb 374 Fishbowl

10:15: Research Seminar: Damian Krysan - Loeb 374 Fishbowl

11:15: Exp. 4: Lobster model of aspergillosis-RNA Extraction and fungal burden qPCR

1-7pm:

Exp 3 Aspergillus transformation –Quantitate Phenotypes

Exp 4: Lobster model of aspergillosis – fungal burden qPCR analysis

Exp 5: Zebrafish – Quantify phenotypes – additional monitoring/injections

7:00: Krysan/Lin Keynote Lecture: Lena Heung–Bay Reading Room

Reception to Follow- Quad picnic area

August 9th, Sunday

Students Day off – *Students organize day off activities*

TAs Exp 6: Candida transformation culture prep

TAs Exp 7: Lobster Candida experiment –day -1 cultures

August 10th, Monday

9:00-10:00: Nuts/Bolts – Exp 6 Candida transformation– Matt Anderson - Loeb 374 Fishbowl

10:15-11:15: Nuts/Bolts Exp 7Candida animal models– David Andes - Loeb 374 Fishbowl

1:00 – 5:30: Laboratory

Exp 6 Candida transformation Day 1: CRISPR transformation

Exp 7 Lobster Candida Inoculation – Tail Veins at ACF in groups

6:00: Jack Edwards Memorial Event on zoom?

7:00: Clinical Mycology – Case Studies – Bay Reading Room

9:00: Exp 7 Lobster Candida model Day 1 – Antifungal Treatments and Monitoring 1

August 11th, Tuesday

8 AM: Exp 6 Candida transformation Day 2: Heat Shock Cells, recovery incubations

9:00 – 10:00: Research Seminar: Matt Anderson -Loeb 374

10:15 -11:15: Research Seminar: David Andes- Loeb 374

11:15: Laboratory: Exp 6 Candida transformation Day 2: Plate Recovery Cells before lunch

11:15 – 12:15:

1-7: Exp 7 Lobster Candida Model Day 2 – Antifungal Treatments and Monitoring 2

5:00: Pizza day- Quad picnic area

7:00: Seminar Julie Segre Bay Reading Room

August 12th, Wednesday

9-10: Research Seminar Bridget Barker

10:15-11:15: Research Seminar: Felipe Santiago-TiradoLoeb 374

1:00: Nuts and BoltsExp 8: Cryptococcus:Macrophages–Felipe Santiago-Tirado

1-7: Laboratory:

Exp 6: Candida transformation Day 3: check plates

Exp 7: Lobster Candida Model Day 3– Antifungal Treatments and Monitoring 3

(potential take down depending on disease progression)

7 – 10: MOMY Mini-Golf Event – Cataumet Mini-Golf (sign up for car rides)

August 13th, Thursday

9 - 10: Research Seminar: Mariana Castanheira - Loeb 374 Fishbowl

10:15: Research Seminar: Tom Chiller -Loeb 374 Fishbowl

11:15:

1-7:

Exp 6 Candida transformation Day 4: patch transformants if present

Exp 7: Lobster Candida Model Day 4 – take down and CFU plating

Exp 8: Cryptococcus Macrophage Cultures – Plate Set Up Interactions

7:00: Fungal Outbreak Exercise Session – Tom Chiller -Bay Reading Room

August 14th, Friday

9-10: Research Seminar: Andy Alspaugh- Loeb 374 Fishbowl

10:15: Research Seminar: Deborah Hogan- Loeb 374 Fishbowl

1-7: Laboratory

Exp 6 Candida transformation Day 4: patch transformants if present

TAs Lab Packing

Exp 7 Lobster Candida Data Analysis CFU counting

Exp 8: Cryptococcus Macrophage Cultures – Image and Analyze Interactions, conduct ELISAs

TAs start Exp 9 Biofilm cultures

7:00: Alspaugh/Hogan Keynote Lecture: Amariliz Rivera

Reception to Follow- Quad picnic area

August 15th, Saturday

9-10: Research Seminar: Rebecca Shapiro- Loeb 374 Fishbowl

10:15: Nuts and Bolts – Exp 9 Candida biofilms Jeniel Nett- Loeb 374

Exp 6 Candida transformation Day 5 – PCR Verification of gene deletion Exp 6 Candida transformation Day 7: PCR Verification #2/integration

Exp 8: Cryptococcus Macrophage Cultures – Analysis and ELISA if needed

Exp 9 Candida biofilm Day 1 - plates for drug leukocyte studies 1PM, TREAT 6P

7:00 PM: TBD (Directions for talks?)

TAs Lab Packing

August 16th, Sunday

8:15: Meet in front of Swope to walk to Lighthouse

9:00: Falmouth Road Race viewing

11:15:

1 PM: Exp 9 Candida biofilm Day 2 –drug treatment and leukocyte-biofilm labeling/imaging

Exp 9 Candida biofilm Day 3 – XTT analysis

Students begin work on Final Presentations

TAs Finalize Lab Packing*

7 PM: Research Seminar: Ted White– Bay Reading Room

August 17th, Monday

9-10: Research Seminar: Joseph Heitman- Loeb 374 Fishbowl

10:15 – 11:15: Research Seminar: Neil Gow - Loeb 374 Fishbowl

130 PM: Student Data presentations – Location Loeb 374 Fish Bowl - 10 MIN PER GROUP

5:30: Closing Banquet Lobster/Chicken/Pasta Dinner –Shuckers, Downtown Woods Hole

7:00: Final Farewells, certificates, social – Loeb Quad picnic area

August 18th, Tuesday

Course Departure for all