This is a draft schedule and will likely be updated.

Time Event Faculty Location

Wednesday, 4/24/2024

1:30 - 2:30 PM

Check in and get picture ID

3:00 - 4:00

Welcome and course orientation

Course Directors, Dir. Ed

Speck Auditorium

4:00 - 4:50

LECTURE and LAB: Ray Optics: Making an image and ray tracing

Mattheyses

Loeb 260 (Main Lab)

5:00 - 6:00

Wine and Cheese reception

Tent outside Swope

6:00 - 7:00

DINNER

7:00 - 7:30

LAB Continued

Mattheyses

Main lab

7:30 - 7:50

LECTURE: Ray optics: Apertures and field stops

Mattheyses

Main lab

7:50 - 8:30

LAB: Group work with irises

Mattheyses/ Commercial Faculty

Main lab

8:30 - 8:45

LECTURE: Ray optics: Building a microscope

Mattheyses

Main lab

8:45 - 9:30

LAB: Building a microscope condenser in Kohler illumination; bulding the imaging path

Mattheyses/ Commercial Faculty

Main lab

9:30 - 9:50

LECTURE: Report out on condenser.  Imaging onto a sensor

Mattheyses

Main lab

9:50 - 10:30

LAB: Building the imaging path; measuring the magnification of your microscope

Mattheyses/ Commercial Faculty

Main lab

Thursday, 4/25/2024

8:45 - 8:50 AM

Daily Overview

Laevsky

Main lab

8:50-10:30

LECTURE: Wave Optics: Infinity-corrected objectives, Diffraction and Resolution; LAB: Measuring the resolution of your microscope

Mattheyses

Main lab

10:35-11:35

What is a digital image? (array of numbers, bit depth, LUTs etc)

Maddox

Speck

11:35 - 12:30

Wave optics, diffraction and contrast generation

Kner

Speck

12:30 - 1:30 PM

LUNCH (students need to be there by 1)

1:30 - 2:30

Optics

Ross

Speck

2:30 - 3:30

Image Processing Lecture: FIJI

Wilson

Speck

3:40-5:30

Image Processing Lab: FIJI

Wilson/Directors

Main lab

5:40 - 6:30

Fourier

Kner

Speck

6:30 - 7:30

Dinner

7:30-8:00

Abbe demonstration

Main lab

8:00 - 10:30

lightning talks

Mattheyses

Main lab

Friday, 4/26/2024

8:45 - 9:00 AM

Daily Overview

Laevsky

Speck

9:00 – 10:00

Phase Contrast & Darkfield Lecture

Mehta

Speck

10:00 - 12:30

Phase Contrast & Darkfield Laboratory

Mehta/Kner/ Commercial Faculty

Main lab

12:30 - 1:30 PM

LUNCH

1:30 - 2:30

Polarization and DIC

Mehta

Speck

2:30 - 4:30

Polarization and DIC Laboratory

Mehta/ Kner

Main Lab

4:30-5:30

PSF/trans/fluor

Laevsky

Speck

5:30-6:30

DINNER

6:30-8:00

Fluorescence Lecture

Salmon

Speck

8:00-11:00

Fluorescence Lab

Salmon

Main lab

Saturday, 4/27/2024

8:45 - 9:00 AM

Daily Overview

Kner

Speck

9:00 - 10:30

Digital Detectors

Watkins

Speck

10:30-12:00

Quant lab

Course Directors

Main lab

12:00-1:00

LUNCH

1:00-3:00

Quant Lab

Course Directors/ Commercial Faculty

Loeb var

3:00-3:30

Quant discussion

Course Directors/ Commercial Faculty

Main lab

3:30-5:30

Noise Lab

Course Directors

Main lab

5:30-6:30

DINNER

6:30-8:00

Fluorescence Proteins and Probes

Palmer

Speck

8:00-10:00

FRET Biosensors Lab

Palmer

Loeb various

10:00-11:00

Vendor Talks

Main Lab

Sunday, 4/28/2024

8:45 - 9:00 AM

Daily Overview

Mattheyses

 

9:00 - 10:00

Quantifying Cell Mechanics

Salaita

Speck

10:00 -10:30

A/O demo

Vincent

Main Lab

10:30 -12:45

Live Cell Lecture & Lab

Maddox

Main Lab

12:45-2 PM

LUNCH

2-5:30

Live Cell

Maddox/Mattheyses

Loeb var

5:30-6

Discussion

Maddox/Mattheyses

Loeb var

6 - 7:00

DINNER

7:00 - 8:00

3D Microscopy I: The Problems and a Wide-Field Solution

Shaw

Speck

8:00- 11:00

Deconvolution Laboratory Exercises

Shaw

Loeb var

Monday, 4/29/2024

8:45 – 9:00 AM

Daily Overview

Mattheyses

Speck

9:00-10:00

3D Microscopy II: Point-scanning Solutions and Their Problems

Shaw

Speck

10:00-12:30

Confocal and Multiphoton Laboratory Exercises

Shaw

Loeb var

12:30-1:30 PM

LUNCH

1:30-2:00

3D Microscopy Discussion I

Shaw

Speck

2:00-3:00

3D Microscopy III: Analysing Performance and Choosing Methods

Shaw

Speck

3:00-5:30

Quantitative Evaluation of Performance in 3D Microscopy

Shaw

Loeb var

5:30-6:30

DINNER

6:30-7:00

3D Microscopy Discussion II

Shaw

Speck

7:00-8:00

TIRF Lecture

Taraska

Speck

8:00-11:00

TIRF Lab

Mattheyses/Taraska

Loeb var

Tuesday, 4/30/2024

8:45 – 9:00 AM

Daily Overview

Kner

Speck

9:00 - 10:00

SR SMM Localization Lecture

Anna-Karin

Speck

10:00 - 11:00

SR SMM Single particle tracking lecture

Lidke

Speck

11:00-12:00

SR SIM/STED lecture

Hesper Rego

Speck

12:00-1:00

Lunch

1:00-5:30

SR lab rotations

Loeb various

5:30-6:30

Dinner

Main lab

6:30-8:30

SR lab rotations

8:30-9:00

SR Discussion

Anna-Karin, Diane, Hesper

Main Lab

9:00 - 10:00

Ethics

Directors

Main lab

Wednesday, 5/1/2024

8:15 - 8:30 AM

Daily Overview

Kner

Main lab

8:30-9:30 AM

LSFM Lecture

Kumar

Speck

9:30-12:00

LSFM Lab

Kumar

Loeb various

12:00 - 1:00 PM

Lunch

1:00-3:00 PM

LSFM Lab

Loeb various

3:00-6:00 PM

FREE TIME

6:00-7:00

Dinner

7:00 - 8:00

Advanced Image Analysis

Beth C. / Simon N.

Speck

8:00 - 10:00

Analysis Lab

Simon N.

Main lab

Thursday, 5/2/24

8:45 - 9:00 AM

Daily Overview

Mattheyses

Main lab

9:00 - 12:00

Machine Learning for Image Processing Laboratory

Cortes

Main lab

12:00 - 1:00 PM

LUNCH

1:00 - 4:00

UC2

UC2

Main lab

4:00 - 5:30

Computer-hardware communication, open source microscope control

UC2

Main lab

5:30 - 6:00

Break

6:00 - 7:00

Inoue Lecture

Margaret A. Titus

Meigs Room

7:00 - 9:00

Clambake

everyone!

Swope Tent

Friday, 5/3/24

Depart MBL