This is a draft schedule and will likely be updated.

Wednesday, 4/26/2023

1:30 - 2:30 PM

Check in and get picture ID

3:00 - 3:50

Welcome and course orientation

Course Directors, Dir. Ed

Speck Auditorium

4:00 - 4:20

LECTURE: Ray Optics: Making an image

Mattheyses

Loeb 260 (Main Lab)

4:20 - 4:50

LAB: Group work with lenses and ray tracing

Mattheyses / Commercial Faculty

Main Lab

5:00 - 6:00

Wine and Cheese reception

Tent outside Swope

6:00 - 7:00

DINNER

7:00 - 7:30

LAB Continued

Mattheyses

Main lab

7:30 - 7:50

LECTURE: Ray optics: Apertures and field stops

Mattheyses

Main lab

7:50 - 8:30

LAB: Group work with irises

Mattheyses / Commercial Faculty

Main lab

8:30 - 8:45

LECTURE: Ray optics: Building a microscope CONDENSER and imaging onto a sensor

Mattheyses

Main lab

8:45 - 9:30

LAB: Group work: Building a microscope CONDENSER in Kohler illumination; building the imaging path

Mattheyses / Commercial Faculty

Main lab

9:30 - 9:50

LECTURE: Report out on condenser. Imaging onto a sensor

Mattheyses

Main lab

9:50 - 10:00

LAB: Building the imaging path; measuring the magnification of your microscope

Mattheyses / Commercial Faculty

Main lab

Thursday, 4/27/2023

8:45 - 8:50 AM

Daily Overview

Maddox

Main lab

8:50-10:00

LECTURE: Wave Optics: Infinity-corrected objectives, Diffraction and Resolution; LAB: Measuring the resolution of your microscope

Mattheyses

Main lab

10:00-10:45

What is a digital image? (array of numbers, bit depth, LUTs etc)

Maddox

10:45 - 12:00

Wave optics, diffraction and contrast generation

Kner

Speck

12:00 - 1:00 PM

LUNCH

1:00 - 2:00

PSF/trans/fluor

Kner

Main lab

2:00 - 3:00

Image Processing Lecture: FIJI

Nelson

3:00-5:00

Image Processing Lab: FIJI

Nelson/Directors

5:00 - 6:00

Fourier

Kner

6:00 - 7:30

Dinner/Abbe

7:30 - 10:30

lightning talks

Friday, 4/28/2023

8:45 - 9:00 AM

Daily Overview

Laevsky

Speck

9:00 – 10:30

Digital Detectors

Watkins

Speck

10:30 - 12:00

Quant Lab

Course Directors / Commercial Faculty

Loeb var

12:00 - 1:00 PM

LUNCH

1:00 - 2:30

Quant Lab

Course Directors / Commercial Faculty

Loeb var

2:30 - 3:00

Quant discussion

Course Directors / Commercial Faculty

Main lab

3:00-6:00

Noise Lab

Course Directors

Main lab

6:00-7:00

Dinner

7:00-8:30

Fluorescence Lecture

Salmon

Speck

8:30-11:00

Fluorescence Lab

Salmon

Main lab

Saturday, 4/29/2023

8:45 - 9:00 AM

Daily Overview

Kner

Speck

9:00 - 10:00

Optics

Ross

Speck

10:00-11:00

Phase Contrast & Darkfield Lecture

Mehta

Speck

11:00-1:00

Phase Contrast & Darkfield Laboratory

Mehta / Kner / Commercial Faculty

Main lab

1:00-2:00 PM

LUNCH

1:00-2:00

Polarization and DIC

Mehta

Speck

2:00-4:00

Polarization and DIC Laboratory

Mehta/ Kner

Main Lab

4:00-5:30

Fluorescence Proteins and Probes (1H)

Palmer

Speck

5:30 - 6:30

DINNER

6:30-7:30

PSF Engineering

Anna-Karin

Speck

7:30-9:30

FRET Biosensors Lab

Palmer

Speck

9:30-10:30

Vendor Talks

Sunday, 4/30/2023

8:45 - 9:00 AM

Daily Overview

Maddox

9:00 - 10

Quantifying Cell Mechanics

Salaita

Speck

10-10:30

Live Cell

Maddox

Speck

10:30-11

COFFEE BREAK

11-12:45

Live Cell

Maddox / Mattheyses

Loeb var

12:45-2 PM

LUNCH

2-5:30

Live Cell

Maddox / Mattheyses

Loeb var

5:30-6

Discussion

Maddox / Mattheyses

Loeb var

6 - 7:00

DINNER

7:00 - 8:00

 

3D Microscopy I: The Problems and a Wide-Field Solution

Shaw

Speck

8:00- 11:00

Deconvolution Laboratory Exercises

Shaw

Loeb var

Monday, 5/1/2023

8:45 – 9:00 AM

Daily Overview

Mattheyses

Speck

9:00-10:00

3D Microscopy II: Point-scanning Solutions and Their Problems

Shaw

Speck

10:00-12:30

Confocal and Multiphoton Laboratory Exercises

Shaw

Loeb var

12:30-1:30 PM

LUNCH

1:30-2:00

3D Microscopy Discussion I

Shaw

Speck

2:00-3:00

3D Microscopy III: Analysing Performance and Choosing Methods

Shaw

Speck

3:00-5:30

Quantitative Evaluation of Performance in 3D Microscopy

Shaw

Loeb var

5:30-6:30

DINNER

6:30-7:00

3D Microscopy Discussion II

Shaw

Speck

7:00-8:00

TIRF Lecture

Taraska

Speck

8:00-11:00

TIRF Lab

Mattheyses / Taraska

Loeb var

Tuesday, 5/2/2023

8:45 – 9:00 AM

Daily Overview

Kner

Speck

9:00 - 10:00

SMM Lecture

Lidke

Speck

10:00-1:00

SMM Lab

Lidke

Loeb var

1:00-2:00

Lunch

2:00-2:30

SMM Discussion

Lidke

Speck

2:30-3:30

LSFM Lecture

Kumar

Speck

3:30-5:30

LSFM Lab

Kumar

Loeb var

5:30-6:30

Dinner

6:30-9:30

LSFM Lab continued

Kumar

Loeb var

Wednesday,5/3/2023

8:45 - 9:00 AM

Daily Overview

Kner

Speck

9:00-10:00

SIM Lecture

Hari

Speck

10:00 - 1:00

SIM Lab

Hari

Loeb var

1:00 - 2:00 PM

Lunch

2:00 - 5:00

FREE TIME

5:00 - 6:00

Advanced Image analysis

Paul-Gilloteaux

Speck

6:00-7:00

Dinner

6:00 - 9:00

analysis lab

Paul-Gilloteaux

Main lab

9:00 - 10:00

ethics

Directors

Main lab

Thursday, 5/4/23

8:45 - 9:00 AM

Daily Overview

Mattheyses

Main lab

9:00 - 12:00

UC2

UC2

Main lab

12:00 - 1:00 PM

LUNCH

1:00 - 2:30

Computer-hardware communication, open source microscope control

UC2

Main lab

2:30 - 5:30

Machine Learning for Image Processing Laboratory

Cortes

Main lab

5:30 - 6:00

Break

6-7pm

Inoue Lecture

Daniel Kiehart

Meigs Room

7:00-9:00

Clambake

Everyone!

Swope Tent