2025 AQLM Schedule

Wednesday, 4/30/25

1:30 - 2:30 PM

Check in and get picture ID

3:00 - 4:00

Welcome and course orientation

Course Directors, Dir. Ed

Speck Auditorium

4:00 - 4:50

LECTURE and LAB: Ray Optics: Making an image and ray tracing

Mattheyses

Loeb 260 (Main Lab)

5:00 - 6:00

Wine and Cheese reception

Tent outside Swope

6:00 - 7:00

DINNER

7:00 - 7:30

LAB Continued

Mattheyses

Main lab

7:30 - 7:50

LECTURE: Ray optics: Apertures and field stops

Mattheyses

Main lab

7:50 - 8:30

LAB: Group work with irises

Mattheyses/ Commercial Faculty

Main lab

8:30 - 8:45

LECTURE: Ray optics: Building a microscope

Mattheyses

Main lab

8:45 - 9:30

LAB: Building a microscope condenser in Kohler illumination; building the imaging path

Mattheyses/ Commercial Faculty

Main lab

9:30 - 9:50

LECTURE: Report out on condenser. Imaging onto a sensor

Mattheyses

Main lab

9:50 - 10:30

LAB: Building the imaging path; measuring the magnification of your microscope

Mattheyses/ Commercial Faculty

Main lab

Thursday, 5/1/25

8:45 - 8:50 AM

Daily Overview

Main lab

8:50-10:30

LECTURE: Wave Optics: Infinity-corrected objectives, Diffraction and Resolution; LAB: Measuring the resolution of your microscope

Mattheyses

Main lab

10:30-11:30

LAB: Kohler on the commercial systems

Mattheyses

Main lab

11:35 - 12:30

Wave optics, diffraction and contrast generation

Kner

Speck

12:30 - 1:30 PM

LUNCH (students need to be there by 1)

1:30 - 2:30

Optics

Ross

Speck

2:30 - 3:30

Image Processing Lecture: FIJI

Bjorn Paulson

Main lab

3:30-5:30

Image Processing Lab: FIJI

Bjorn Paulson

Main lab

5:30 - 6:30

Fourier

Kner

Speck

6:30 - 7:30

Dinner

7:30-10:00

Lightning Talks

Mattheyses

Main lab

Friday, 5/2/25

8:45 - 9:00 AM

Daily Overview

Speck

9:00 – 10:00

Label free: Phase/Pol/Darkfield

Shalin Mehta

Speck

10:00 - 12:00

Label free: Phase/Pol/Darkfield lab

Shalin Mehta

Main lab

12:00 - 1:00 PM

LUNCH

1:00 - 2:00

Label free: Phase/Pol/Darkfield lab (con't)

Shalin Mehta

2:00 - 3:00

Abbe demonstration and discussion

3:00-4:00

PSF/trans/fluor

Laevsky

4:00-5:30

Fluorescence Lecture

Salmon

Speck

5:30-6:30

DINNER

6:30-10:00

Fluorescence Lab

Salmon

Main lab

Saturday, 5/3/25

8:45 - 9:00 AM

Daily Overview

Speck

9:00 - 10:30**

Digital Detectors

Watkins

Speck

10:30-12:00

Noise lab

Course Directors/Commercial Faculty

Main lab

12:00-1:00

LUNCH

1:00-2:00

Noise Lab con't

Course Directors/ Commercial Faculty

Loeb var

2:00-5:00

Quant lab

Course Directors/ Commercial Faculty

Main lab

5:00-5:30

Noise and Quant discussion

Course Directors

Main lab

5:30-6:30

DINNER

6:30-8:00

Fluorescence Proteins and Probes

Palmer

Speck

8:00-10:00

FRET Biosensors Lab

Palmer

Loeb var

10:00-11:00

Vendor Talks

Main Lab

Sunday, 5/4/25

8:45 - 9:00 AM

Daily Overview

Speck

9:00 - 10:00

Quantifying Cell Mechanics

Salaita

Speck

10:00 -10:30

BREAK

Main Lab

10:30 -12:00

Live Cell and labeling lecture

Bartle

Main Lab

12:00-1:00 PM

LUNCH

1-5:30

Live Cell and Labeling lab

Bartle

Loeb var

5:30-6

Live Cell Discussion

Bartle

Loeb var

6 - 7:00

DINNER

7:00 - 8:00

3D Microscopy I: The Problems and a Wide-Field Solution

Shaw

Speck

8:00- 11:00

Deconvolution Lab

Shaw

Loeb var

Monday, 5/5/25

8:45 – 9:00 AM

Daily Overview

Speck

9:00-10:00

3D Microscopy II: Point-scanning Solutions and Their Problems

Shaw

Speck

10:00-12:30

Confocal Laboratory Exercises

Shaw

Loeb var

12:30-1:30 PM

LUNCH

1:30-2:00

3D Microscopy Discussion I

Shaw

Speck

2:00-3:00

3D Microscopy III: Analysing Performance and Choosing Methods

Shaw

Speck

3:00-5:30

Quantitative Evaluation of Performance in 3D Microscopy

Shaw

Loeb var

5:30-6:30

DINNER

6:30-7:00

3D Microscopy Discussion II

Shaw

Speck

7:00-8:00

TIRF Lecture

Taraska

Speck

8:00-11:00

TIRF Lab

Taraska/Mattheyses

Loeb var

Tuesday, 5/6/25

8:45 – 9:00 AM

Daily Overview

Speck

9:00 - 10:00

SR SMM Localization Lecture

Gustavsson

Speck

10:00 - 11:00

SR SMM Single particle tracking lecture

Lidke

Speck

11:00-12:00

SR SIM/STED lecture

Bahlmann

Speck

12:00-1:00

Lunch

1:00-2:00

Vincent A/O demo

2:00-5:30

SR LAB

Gustavsson, Lidke

Loeb various

5:30-6:30

Dinner

6:30-10:30

SR lab

Gustavsson, Lidke

10:30-11:00

SR Discussion

Gustavsson, Lidke

Main Lab

Wednesday, 5/7/25

8:45 - 9:00 AM

Daily Overview

Speck

9:00-10:00

LSFM Lecture

Laevsky

Speck

10:00-12:00

LSFM Lab

Laevsky

Loeb various

12:00 - 1:00 PM

Lunch

1:00-3:00

LSFM Lab

Loeb various

3:00-6:00

FREE TIME

6:00-7:00

Dinner

7:00 - 8:00

Advanced Image Analysis Lecture

Li Liu

Speck

8:00 - 10:00

Analysis Lab

Li Liu

Main lab

10:00-11:00

Ethics

Directors

Thursday, 5/8/25

8:45 - 9:00 AM

Daily Overview

Main lab

9:00 - 12:00

Machine Learning for Image Processing Laboratory

Cortes

Main lab

12:00 - 1:00 PM

LUNCH

1:00 - 4:00

UC2

UC2

Main lab

4:00 - 5:30

Computer-hardware communication, open source microscope control

UC2

Main lab

5:30 - 6:00

Break

6:00 - 7:00

Inoue Lecture

Sophie Brasselet

Meigs Room

7:00 - 9:00

Clambake

Everyone!

Swope Tent

Friday, 5/9/25

Depart MBL