MRC Services

The purpose of this application is twofold: (1) to ensure that researchers receive maximum benefit from the MRD services, and (2) to ensure that you are aware of the policies and procedures to be followed while using MBL aquatic holding facilities. This application will be reviewed by the MRD Manager, and semi-annually (April and November) thereafter. Questions should be directed to tanks@mbl.edu.

Use of vertebrates at the MBL is regulated by the MBL Institutional Animal Care and Use Committee (IACUC) and requires an approved IACUC protocol.  Vertebrates (e.g., fish) may be ordered from MBL and shipped to qualified research and educational institutions without MBL IACUC requirements but may be subject to their own institutional vertebrate use regulations.  

Before ordering animals for use at MBL, please refer to the IACUC protocols page. The forms below can be found on that page.

• Vertebrate Animals Use Application
• Aquatic Vertebrate Order Form
• Mammal Order Form

All research involving the use of zebrafish must have a protocol approved by the MBL Institutional Animal Care and Use Committee (IACUC).

Investigators using zebrafish supplied by the MBL Zebrafish Facility will need to refer to the Zebrafish Facility Guidelines for more details and per diem charges.

The Marine Resource facility’s sophisticated life support and water treatment systems enhance both the quality of organisms provided and the diversity of research that can be conducted within the building. As many as ten different regimens of seawater can be provided simultaneously to any of the research or animal holding areas. In addition to raw seawater and a filtered/ozonated water supply, eight independent recirculating seawater systems provide flexible tailoring for almost any aquatic environment Recirculated seawater is ozone-sanitized to control fouling organisms and facultative pathogens. Accurate temperature and photoperiod control within the building allow for the manipulation of the reproductive state of organisms.

Fees for Tank Space and Facilities Use
Visiting researchers are generally exempt from fees for tank space and facility use. Researchers are expected to take daily care of their animals and regularly feed and clean tanks or enclosures assigned to them. Support by MRC staff is available at $40/hour.

The MRC was designed for versatility and efficiency to maximize the health and longevity of the aquatic organisms that are the lifeblood of research at the Marine Biological Laboratory (MBL). Keeping aquatic organisms alive and available to scientists year-round is crucial to research at the MBL. To ensure reliability and to safeguard organisms being cultured or maintained in the MRC, all incoming seawater supply lines, filtration systems, and treated-water heat exchangers are redundant. All pumps, temperature sensors, and the ozone distribution system are alarmed and under computer control. Water temperatures are continuously monitored and adjusted every 4 seconds by computer to ensure a temperature stability of 0.5oC. The system is also remotely accessible by portable computer and telephone modem and is backed by staff on call 24 hours a day. Any failure in the system is immediately detected and, because of the system’s redundancies, is rapidly corrected to ensure no major losses of organisms. The entire system is supported by routine security checks as well as an emergency 500-kW generator.

The MRC’s ground floor houses a variety of healthy organisms in seawater tanks, and a procedures room for handling, examining, and preparing aquatic organisms. A large portion of the floor space is available to scientists conducting individual research projects.

The second floor contains offices, a classroom, as well as dry and wet laboratories. In-house veterinary services are provided by the MBL staff veterinarian. The floor includes a research holding room for injured or diseased organisms, a necropsy room, histology and microbiology/parasitology labs, a water quality/chemistry laboratory, a mariculture facility, and zebrafish facility.

The third floor contains the mechanical equipment that is crucial to the life support systems of the MRC plus additional laboratory space and offices.

The Marine Resource Department (MRD) staff conducts routine chemical analyses on all seawater systems on the Marine Biological Laboratory campus. These analyses include salinity, pH, conductivity, dissolved oxygen, turbidity, as well as nitrogen compounds (ammonia, nitrite, nitrate), phosphate, sulfate, and some cations. The biochemical stability of the recirculating and treated seawater systems is assured by daily monitoring of the water systems within the Marine Resources facility.

The Veterinary Services Department of the MBL is responsible for the health of both aquatic and terrestrial species of animals held at the MBL. We provide animal information, routine veterinary care and respond to animal health problems throughout the MBL. Investigators are encouraged to contact the Attending Veterinarian with any questions about care, housing, disease, appropriate animal models, and handling, as well as protocol pre-review, surgical and other experimental techniques.

Facilities
Our facilities consist of:

• Mammalian animal housing
• Limited housing for avians, reptiles, and amphibians
• Veterinarian’s office, MRC 311
• Clinical laboratory, MRC 206

Services
Veterinary consultation is provided at the request of the investigator or their technicians. Services provided are heath certifications, veterinary clinical services, and veterinary pathological services.

Contacts
Attending Veterinarian: Lisa Abbo, DVM, ext. 7522; E-mail: vet@mbl.edu
Animal Care Coordinator: Daniel Johnson, ext. 7288; E-mail: djohnson@mbl.edu

Institutional Animal Care and Use Committee (IACUC)
Before any vertebrate animal is used in research at the MBL, a protocol for its usage must be approved by the IACUC. Protocol forms and information can be obtained from the IACUC web site. Questions concerning filling out protocols, current protocol status, or post-approval modifications can be addressed to iacuc@mbl.edu.

ALL ORDERS ARE SUBJECT TO THE TERMS AND CONDITIONS LISTED BELOW.

RUSH CHARGE

We require a minimum of two week's notice for any shipment. A $50 rush fee will be added to any order within a 7-day delivery date.

SPECIAL COLLECTIONS FEE

We do our best to keep a variety of each species readily available to ship, however, some species require more time and effort to collect. We reserve the right to add a Special Collections Fee of $25. 

SUBSTITUTIONS

If we are unable to supply the organisms requested, we may substitute closely related organisms. If you do not wish to have any substitutions, please specify “No Substitutions”.

PRICES

All prices are subject to change without notice. We reserve the right to increase the cost of organisms due to increased costs of collecting or handling.

Service Charges
$46.50 sexing fee when specifying males and/or females.
$66.40 fee for all shipments leaving the continental United States.
$94.00 Veterinary Health Certificate (required for all foreign shipments)
$66.40 minimum lab fee (tissue harvesting)
Additional fees may be added to shipments that require special handling, procedures or supplies.
Price quotes will be honored for 30 days only, from the date of quote.

DELIVERY DATES

We require a two-week notice before expected delivery date. Shipments are made on Mondays, Tuesdays, and Wednesdays. We do not ship on celebrated holidays or for arrival on celebrated holidays or weekends.

DOMESTIC SHIPMENTS

Live organism shipments within the continental United States are via Federal Express – Priority Overnight, for next day delivery unless otherwise requested. Containers of seawater are shipped via FedEx Ground Delivery unless otherwise requested (we will ship by air at the customer’s written request).

All domestic shipping charges will be added to the invoice.

INTERNATIONAL SHIPMENTS

All international shipments charged to a customer-supplied Federal Express account.
Customer assumes full responsibility for all shipments accepted and handled by third party brokers. MBL is not responsible for any delivery delays due to customs issues outside of our control. Any organisms that arrive DOA within the expected delivery time can be replaced free of charge.

REPLACEMENT SPECIMENS

Specimens suffering mortality or morbidity and need replacement may be requested within two (2) days of delivery. Replacements requested within this two-day period will be supplied at no cost, and shipped at no cost to the customer. Replacement requests made after two days will be handled as new orders and the customer will assume responsibility for the costs of specimens and shipping.

SHIPPING DURING SEVERE TEMPERATURES OR WEATHER CONDITIONS

Customer assumes full responsibility for all shipments made when severe weather is anticipated and during periods of severe temperatures and adverse weather.

CONTAINERS AND PACKAGING

If the customer requests specific packaging methods, which differ from the established methods employed by the Marine Resources Department, the customer assumes full responsibility for the condition of the organisms upon delivery.

All shipping containers and packing material become the property of the customer, the cost added to the invoice. Containers are not returnable. No credit issued for the return of containers.

Containers used to ship seawater become the property of the customer, the cost to be added to the invoice. Containers may be returned for refill at time of placing subsequent orders for seawater. A nominal-handling fee may be charged. Do not return containers to us without placing an order for seawater.

INVOICING

Invoices will be generated by the MBL’s Accounts Receivable department at the close of each month and sent via postal mail. If you require a purchase order (PO) number on your invoice, it must be provided at the time your order is placed.

PAYMENT OF OUR INVOICES

Payment terms are net 30. Instructions to submit payment via check, credit card and bank transfer are provided on invoices and statements.

QUESTIONS CONCERNING YOUR ORDER OR INVOICE

Difficulties with order, shipment or invoices please notify us as soon as possible.
Direct questions concerning your order or invoice to:

Email MRD

Marine Biological Laboratory
Marine Resources Department
7 MBL Street
Woods Hole, Massachusetts 02543