This system facilitates illuminating samples using a linearly polarized light in Epi or TIRF mode and detects fluorescence emission in parallel and perpendicular directions using a single sCMOS camera. Anisotropy imaging can provide information about protein-protein interaction or protein clustering.
Commercial or Pre-commercial:
Imaging Innovation Initiative
405 nm, 488 nm, 561 nm, 640 nm
A single band pass filter appropriate for the illumination.
100x, 1.49 NA Oil TIRF lens
60x, 1.4 NA Oil lens
Microscope Control software:
Custom designed Graphical User Interface in Matlab
Image Analysis Software:
Custom designed Image Analysis Graphical User Interface in Matlab