dual view line scanning confocal
General Overview: A custom-built line scanning confocal microscope with two opposing views that is capable of imaging samples up to several millimeters in length at high resolution and fast speed.
Commercial or Pre-commercial: Pre-Commercial
Which Facility: Imaging Innovation Center
Imaging Mode: Optical
If Optical: Confocal
Microscope Stand: 4-pi configuration custom designed
Features: Capable of imaging large areas and volumes rapidly with high NA objective lenses.
Simultaneous two-sided illumination and detection to increase volumetric speed and obtain sample information that may be occluded from a single view.
Laser Wavelengths: 405 nm, 488 nm, 561 nm, 640 nm
Excitation/Emission Filters: Quad band filter 405/488/561/640 lasers. A filter wheel with an individual band bass filter for each laser excitation wavelength.
Detector: Camera
Imaging lenses: 25x, 1.1 NA Water Immersion
40x, 1.25 NA Silicone
60x, 1.4 NA Oil
100x, 1.35 NA Silicone
100x, 1.49 NA Oil
Microscope Control software: Custom designed Graphical User Interface in Matlab
Image Analysis Software: Custom designed in Matlab
CO2: No 
Humidity: No
Light Tight: Yes
Sample mounting requirements/constraints: Convention sample mounting approaches. Glass Bottom Petridish
Location - Building and Room: Lillie 219
Contact Person: Abhishek Kumar
Total Internal Reflection Fluorescence (TIRF) Microscope
General Overview: Home-built TIRF Microscope capable of illuminating samples in a ring-TIR mode or a single sided TIR.
Commercial or Pre-commercial: Pre-Commercial
Which Facility: Imaging Innovation Center
Imaging Mode: Optical
If Optical: Widefield/TIRF
Microscope Stand: Inverted
Features: Epi illumination or TIR illumination
Laser Wavelengths: 405 nm, 488 nm, 561 nm, 640 nm
Excitation/Emission Filters: Quad band filter 405/488/561/640 lasers. Individual emission filters can be added manually.
Detector: Camera
Imaging lenses: 100x, 1.49 NA TIRF lens
Microscope Control software: Custom designed Graphical User Interface in Matlab and u-Manager
Image Analysis Software:  
CO2: No
Humidity: No
Light Tight: Yes
Sample mounting requirements/constraints: Conventional sample mounting.
Location - Building and Room: Lillie 219
Hourly Rate for Use: $25.00
Contact Person: Abhishek Kumar
General Overview: A home built dual-view SPIM system using multi-immersion lenses for imaging clear brain tissues. This system is also suitable for imaging samples in water/sea water.
Commercial or Pre-commercial: Pre-Commercial
Which Facility: Imaging Innovation Center
Imaging Mode: Optical
If Optical: Lightsheet
Microscope Stand: Custom designed frame
Features: Suitable for imaging large samples at high volumetric speed.
Laser Wavelengths: 405 nm, 488 nm, 561 nm, 640 nm
Excitation/Emission Filters: Quad band filter for 405/488/561/640 lasers.
Detector: Camera
Imaging lenses: 14x, 0.4 NA Multi-immersion lenses
23x, 0.7 NA Multi-immersion lenses
Microscope Control software: Custom designed Graphical User Interface in Matlab
Image Analysis Software: Fiji/Matlab
CO2: No
Humidity: No
Light Tight: No
Sample mounting requirements/constraints: Conventional sample mounting
Location - Building and Room: Lillie 219
Contact Person: Abhishek Kumar
Anisotropy Imaging System
General Overview: This system facilitates illuminating samples using a linearly polarized light in Epi or TIRF mode and detects fluorescence emission in parallel and perpendicular directions using a single sCMOS camera. Anisotropy imaging can provide information about protein-protein interaction or protein clustering. 
Commercial or Pre-commercial: Pre-Commercial
Which Facility: Imaging Innovation Center
Imaging Mode: Optical
If Optical: Widefield/TIRF
Microscope Stand: Inverted
Features: 405 nm, 488 nm, 561 nm, 640 nm
Laser Wavelengths: A single band pass filter appropriate for the illumination.
Excitation/Emission Filters:  
Detector: Camera
Imaging lenses: 100x, 1.49 NA Oil TIRF lens
60x, 1.4 NA Oil lens
Microscope Control software: Custom designed Graphical User Interface in Matlab
Image Analysis Software: Custom designed Image Analysis Graphical User Interface in Matlab
CO2: No
Humidity: No
Light Tight: Yes
Sample mounting requirements/constraints: Conventional sample mounting
Location - Building and Room: Lillie 219
Contact Person: Abhishek Kumar