Microscopes in Development
| General Overview: | A custom-built line scanning confocal microscope with two opposing views that is capable of imaging samples up to several millimeters in length at high resolution and fast speed. |
| Commercial or Pre-commercial: | Pre-Commercial |
| Which Facility: | Imaging Innovation Center |
| Imaging Mode: | Optical |
| If Optical: | Confocal |
| Microscope Stand: | 4-pi configuration custom designed |
| Features: | Capable of imaging large areas and volumes rapidly with high NA objective lenses. Simultaneous two-sided illumination and detection to increase volumetric speed and obtain sample information that may be occluded from a single view. |
| Laser Wavelengths: | 405 nm, 488 nm, 561 nm, 640 nm |
| Excitation/Emission Filters: | Quad band filter 405/488/561/640 lasers. A filter wheel with an individual band bass filter for each laser excitation wavelength. |
| Detector: | Camera |
| Imaging lenses: | 25x, 1.1 NA Water Immersion 40x, 1.25 NA Silicone 60x, 1.4 NA Oil 100x, 1.35 NA Silicone 100x, 1.49 NA Oil |
| Microscope Control software: | Custom designed Graphical User Interface in Matlab |
| Image Analysis Software: | Custom designed in Matlab |
| CO2: | No |
| Humidity: | No |
| Light Tight: | Yes |
| Sample mounting requirements/constraints: | Convention sample mounting approaches. Glass Bottom Petridish |
| Location - Building and Room: | Lillie 219 |
| Contact Person: | Abhishek Kumar |
| General Overview: | Home-built TIRF Microscope capable of illuminating samples in a ring-TIR mode or a single sided TIR. |
| Commercial or Pre-commercial: | Pre-Commercial |
| Which Facility: | Imaging Innovation Center |
| Imaging Mode: | Optical |
| If Optical: | Widefield/TIRF |
| Microscope Stand: | Inverted |
| Features: | Epi illumination or TIR illumination |
| Laser Wavelengths: | 405 nm, 488 nm, 561 nm, 640 nm |
| Excitation/Emission Filters: | Quad band filter 405/488/561/640 lasers. Individual emission filters can be added manually. |
| Detector: | Camera |
| Imaging lenses: | 100x, 1.49 NA TIRF lens |
| Microscope Control software: | Custom designed Graphical User Interface in Matlab and u-Manager |
| Image Analysis Software: | |
| CO2: | No |
| Humidity: | No |
| Light Tight: | Yes |
| Sample mounting requirements/constraints: | Conventional sample mounting. |
| Location - Building and Room: | Lillie 219 |
| Hourly Rate for Use: | $25.00 |
| Contact Person: | Abhishek Kumar |
| General Overview: | A home built dual-view SPIM system using multi-immersion lenses for imaging clear brain tissues. This system is also suitable for imaging samples in water/sea water. |
| Commercial or Pre-commercial: | Pre-Commercial |
| Which Facility: | Imaging Innovation Center |
| Imaging Mode: | Optical |
| If Optical: | Lightsheet |
| Microscope Stand: | Custom designed frame |
| Features: | Suitable for imaging large samples at high volumetric speed. |
| Laser Wavelengths: | 405 nm, 488 nm, 561 nm, 640 nm |
| Excitation/Emission Filters: | Quad band filter for 405/488/561/640 lasers. |
| Detector: | Camera |
| Imaging lenses: | 14x, 0.4 NA Multi-immersion lenses 23x, 0.7 NA Multi-immersion lenses |
| Microscope Control software: | Custom designed Graphical User Interface in Matlab |
| Image Analysis Software: | Fiji/Matlab |
| CO2: | No |
| Humidity: | No |
| Light Tight: | No |
| Sample mounting requirements/constraints: | Conventional sample mounting |
| Location - Building and Room: | Lillie 219 |
| Contact Person: | Abhishek Kumar |
| General Overview: | This system facilitates illuminating samples using a linearly polarized light in Epi or TIRF mode and detects fluorescence emission in parallel and perpendicular directions using a single sCMOS camera. Anisotropy imaging can provide information about protein-protein interaction or protein clustering. |
| Commercial or Pre-commercial: | Pre-Commercial |
| Which Facility: | Imaging Innovation Center |
| Imaging Mode: | Optical |
| If Optical: | Widefield/TIRF |
| Microscope Stand: | Inverted |
| Features: | 405 nm, 488 nm, 561 nm, 640 nm |
| Laser Wavelengths: | A single band pass filter appropriate for the illumination. |
| Excitation/Emission Filters: | |
| Detector: | Camera |
| Imaging lenses: | 100x, 1.49 NA Oil TIRF lens 60x, 1.4 NA Oil lens |
| Microscope Control software: | Custom designed Graphical User Interface in Matlab |
| Image Analysis Software: | Custom designed Image Analysis Graphical User Interface in Matlab |
| CO2: | No |
| Humidity: | No |
| Light Tight: | Yes |
| Sample mounting requirements/constraints: | Conventional sample mounting |
| Location - Building and Room: | Lillie 219 |
| Contact Person: | Abhishek Kumar |