2023 Zebrafish Schedule

Thurs 27 Jul

2:00: – 5:00: Student check-in (Swope)

6:00: – 7:00: Light dinner – cold cuts & veggies (Tent in Loeb Quad)

7:00: – 7:15: Welcome to the Zebrafish Course: Tom Schilling and Debbie Yelon (Speck Auditorium)

7:15: – 8:15: Overview: Zebrafish Development and Genetics: Lila Solnica-Krezel (Speck Auditorium)

8:15: – 9:00: Student 3-min oral presentations (Speck Auditorium)

9:00: – 10:00: Set up posters and locate individual student stations in lab (Loeb 260)

Fri 28 Jul

8:30: – 8:45: Welcome to the MBL: Linda Hyman, Director of Education (Loeb 260)

8:45: – 9:15: Introduction to stereoscopes, iPad/camera & server: Dave Raible and faculty (Loeb 260)

9:15: – 12:00: Pit Talk & Lab: Zebrafish development (temporal dynamics, tissue organization, early embryo anatomy): Lila Solnica-Krezel, Dave Raible (Loeb 260)

12:00: – 1:30: Faculty (Lila Solnica-Krezel and Dave Raible) lunch with students (Swope)

1:30: – 2:30: Follow up on embryos from morning session: Solnica-Krezel and Raible (Loeb 260)

2:30: – 3:30: Lecture: Introduction to Microscopy: Andres Collazo (Loeb 260)

3:30: – 6:00: Hands-on microscopy – practice Kohler illumination, learn Zen software: Andres Collazo, Jim Fadool, Dave Raible, Tom Schilling (Loeb 260)

7:30: – 9:00: Research Lectures & Discussion: Dave Raible and Lila Solnica-Krezel (Speck Auditorium)

9:00: – 9:30: Follow up on embryos and wrap-up from morning session: Solnica-Krezel

9:30: – 11:30: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 1)

9:30: – 11:30: Overnight time-lapse microscopy: Jan Huisken (Group 4)

9:30: – 11:00: Poster Perusal: Groups 2 & 3 (Loeb Hallways)

9:30: – 11:00: Work on t-shirt design

Sat 29 Jul

8:30: – 9:30: Pit Talk: Larval anatomy: Tom Schilling (Loeb 260)

9:30: – 12:00: Larval anatomy – touring larvae with fluorescent transgenics: Collazo, Fadool, Raible, Schilling (Loeb 260)

12:00: – 1:30: Faculty (Becky Burdine and Jan Huisken) lunch with students (Swope)

1:30: – 6:00: Pit Talk & Lab: Adult Dissection and Regeneration (fin and heart): Ken Poss (Loeb 260)

7:30: – 9:00: Research Lectures & Discussion: Andi Pauli and Ken Poss (Speck Auditorium)

9:00: – 11:00: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 2)

9:00: – 11:00: Overnight time-lapse microscopy: Jan Huisken (Group 3)

9:00: – 11:00: Poster Perusal: Groups 1 & 4 (Loeb Hallways)

9:00: – 11:00: Work on t-shirt design

Sun 30 Jul

8:30: – 12:00: Pit Talk & Lab: mRNA and CRISPR injections: Andi Pauli and Becky Burdine (Loeb 260)

12:00: – 1:30: Faculty (Tom Schilling and Ken Poss) lunch with students (Swope)

1:30: – 2:30: Review first set of time-lapses (Groups 1, 2, 3, 4): Fadool, Collazo, Huisken (Loeb G70)

2:30: – 3:30: Workshop: Developmental Genetics: Mary Mullins (Loeb G70)

3:30: – 6:00: Workshop: Zebrafish Genome Literacy, Part 1: Elisabeth Busch-Nentwich (Loeb 260)

7:30: – 9:00: Research Lectures & Discussion: Becky Burdine and Elisabeth Busch-Nentwich (Speck Auditorium)

9:00: – 9:30: Wrap-up mRNA injected embryos: Andi Pauli and Becky Burdine (Loeb 260)

9:30: – 11:30: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 3)

9:30: – 11:30: Overnight time-lapse microscopy: Jan Huisken (Group 1)

9:30–11:00: Poster Perusal: Groups 2 & 4 (Loeb Hallways)

9:30: – 11:00: Work on t-shirt design

Mon 31 Jul

9:00: – 12:00: Pit Talk & Lab: Morpholino & CRISPR injections: Andi Pauli and Becky Burdine (Loeb 260)

11:00: – 12:00: Needle pulling demo: Adair Oesterle (Sutter Instruments) (Loeb 260)

12:00: – 1:30: Faculty (Andres Collazo and Mary Mullins) lunch with students (Swope)

1:30: – 2:00: Review second set of time-lapses (Groups 1 and 3): Fadool, Collazo, Huisken (Loeb G70)

2:00: – 3:00: Workshop: Mary's second workshop (title TBD): Mary Mullins (Loeb G70)

3:00: – 5:00: Workshop: Zebrafish Genome Literacy, Part 2: Elisabeth Busch-Nentwich (Loeb G70)

5:00: – 6:00: Lecture: Light Sheet Microscopy: Jan Huisken (Loeb 263)

7:30: – 9:00: Research Lectures & Discussion: Mary Mullins and Sharon Amacher (Speck Auditorium)

9:00: – 11:00: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 4)

9:00: – 11:00: Overnight time-lapse microscopy: Jan Huisken (Group 2)

9:00: – 11:00: Poster Perusal: Groups 1 & 3 (Loeb Hallways)

9:00: – 11:00: Work on t-shirt design

11:00: T-shirt design is due by 11:00: pm on July 31

Tues 1 Aug

8:30: – 9:00: Introduction to ZFIN, atlases, and web resources: Corinne Houart and Sharon Amacher (Loeb 260)

9:00: – 9:30: Pit Talk: Immunostaining & in situ hybridization: Houart and Amacher (Loeb 260)

9:30: – 12:00: Mounting, analysis, and imaging of fixed embryos: Houart and Amacher (Loeb 260)

12:00: – 1:30: Faculty (Andi Pauli and Elisabeth Busch-Nentwich) lunch with students (Swope)

1:30: – 2:00: Review second set of time-lapses (Groups 2 and 4): Fadool, Collazo, Huisken (Loeb G70)

2:00: – 6:00: Lab: Continuation from morning and make presentations of resulting images (Loeb 260)

6:30: – 7:30: Faculty Meeting (Memorial Circle)

7:30: – 10:00: Faculty & Staff Event; Student self-organized social time and/or assembly of images for next day's wrap-up

Wed 2 Aug

8:30: – 10:30: Wrap-up: Immunostaining & in situ hybridization: student presentations with Houart and Amacher (Clapp Auditorium)

10:30: – 12:00: Workshop: Zebrafish in Education: Roshan Jain, Pierre Le Pabic, and Sharon Amacher (Clapp Auditorium)

12:00: – 1:30: Faculty (Sharon Amacher and Holger Knaut) lunch with students (Swope)

1:30: – 2:30: Check on regenerating fins: Poss and Yelon (Loeb 260)

2:30: – 5:30: Free Time!

5:30: – 6:30: Student/Faculty Challenge (Scavenger Hunt)

6:30: – 9:00: Course BBQ (Loeb Quad)

Thurs 3 Aug

9:00: – 10:30: Workshop: Responsible Conduct in Research: Schilling and Yelon (Loeb 263)

10:30: – 12:00: Pit Talk: Chimeric Analysis: Jim Fadool and Holger Knaut (Loeb 260)

12:00: – 1:30: Faculty (Corinne Houart and Debbie Yelon) lunch with students (Swope)

1:30: – 6:00: Lab: Chimeric analysis – Blastula transplants: Andreas Collazo, Jim Fadool, Holger Knaut, and Tom Schilling (Loeb 260)

7:30: – 9:00: Research Lectures & Discussion: Corinne Houart and Holger Knaut (Speck Auditorium)

9:00: – 11:00: Optional overnight time-lapse microscopy of transplants (on your own)

Fri 4 Aug

8:30: – 9:30: Pit Talk: “High resolution” transplants: Corinne Houart (Loeb 260)

9:30: – 12:00: Lab: “High resolution” transplants (Houart; small groups rotate) and imaging early transplants (Collazo, Fadool, Knaut, and Schilling) (Loeb 260)

12:00: – 1:30: Faculty (Anna Huttenlocher and Alex Schier) lunch with students (Swope)

1:30: – 5:00: Continuation of transplant lab (Houart) and imaging early transplants (Collazo, Fadool, Knaut, and Schilling) (Loeb 260)

5:00: – 6:00: Workshop: When should I (not) use zebrafish as a model system?: Alex Schier (Starr 209)

7:30: – 9:00: Research Lectures & Discussion: Anna Huttenlocher and Kristen Kwan (Speck Auditorium)

9:00: – 11:00: Additional imaging of transplants; assembly of images for transplant wrap-up

Sat 5 Aug

8:30: – 10:30: Wrap-up: Chimeric analysis: student presentations with Collazo, Houart, Fadool, Knaut, and Schilling (Starr 209)

10:30: – 12:00: Pit Talk: Tracking migratory cells in vivo: Anna Huttenlocher (Loeb 260)

12:00: – 1:30: Faculty (Isaac Bianco and Kristen Kwan) lunch with students (Swope)

1:30: – 6:00: Lab: Tracking migratory cells in vivo: Anna Huttenlocher (Loeb 260)

7:30: – 9:00: Research Lectures & Discussion: Isaac Bianco and Jim Fadool (Speck Auditorium)

9:00: – 11:00: Fin regeneration results and terminal imaging: Poss and Yelon (Loeb 260)

Sun 6 Aug

8:30: – 9:30: Wrap-up of tracking migratory cells: Anna Huttenlocher (Speck Auditorium)

9:30: – 10:30: Workshop: Disease modeling in zebrafish: Anna Huttenlocher (Speck Auditorium)

10:30: – 12:00: Pit Talk: Monitoring morphogenesis: Kristen Kwan (Speck Auditorium)

12:00: – 1:30: Faculty (Sarah Kucenas and Katie Kindt) lunch with students (Swope)

1:30: – 5:00: Lab: Monitoring morphogenesis: Kristen Kwan (Loeb 260)

5:00: – 6:00: Workshop: Single-cell and Spatial Transcriptomics in Zebrafish: Alex Schier (Loeb G70)

7:30: – 9:00: Chi-Bin Chien Memorial Research Lecture: Katie Kindt (Speck Auditorium), followed by reception (Tent in Loeb Quad)

Mon 7 Aug

8:30: – 9:30: Wrap-up of monitoring morphogenesis: Kristen Kwan (Starr 209)

9:30: – 10:30: Pit Talk: Behavior: Isaac Bianco and Jim Fadool (Loeb 260)

10:30: – 12:30: Lab: Behavior: Isaac Bianco, Jim Fadool, and John Dowling (Loeb 260)

12:30: – 2:00: Faculty (Jim Fadool and John Dowling) lunch with students (Swope)

2:00: – 5:00: Lab, continued: Behavior: Bianco, Fadool, and Dowling (Loeb 260)

5:00: – 6:00: Workshop: Transgenesis: Sarah Kucenas (Loeb 263)

7:30: – 9:00: Research Lectures & Discussion: Sarah Kucenas and Alex Schier (Speck Auditorium)

10:00: Stony Beach evening quiet field trip to see bioluminescence

Tues 8 Aug

8:30: – 9:30: Pit Talk: Optical Manipulation: Sarah Kucenas and Debbie Yelon (Loeb 260)

9:30: – 12:30: Lab Rotations: Photoconversion: Yelon, Ablation/Axotomy: Kucenas (Loeb 260) AND Fish facility tours

12:30: – 2:00: Faculty (Roshan Jain and Pierre Le Pabic) lunch with students (Swope)

2:00: – 4:00: Lab Rotations: Photoconversion: Yelon, Ablation/Axotomy: Kucenas (Loeb 260) AND Aquaculture vendors

4:00: – 6:00: Workshop: Zebrafish Husbandry and Breeding: April Freeman (Loeb 260)

7:30: – 9:00: Research Lectures & Discussion: Debbie Yelon and Tom Schilling (Speck Auditorium)

9:00: – 11:00: Finish up photo contest submissions and 5-minute student presentations of favorite data

11:00: Photo contest images (individual & group entries) due by 11:00: pm on August 8

Wed 9 Aug

9:00: – 12:00: Cardiac regeneration results and terminal imaging: Poss and Yelon (Loeb 260

12:00: – 1:30: Lunch (Swope)

1:30: – 3:30: Five-minute student presentations of favorite data (Loeb G70)

3:30: – 5:00: Course Wrap-up: discussion with Schilling and Yelon (Tent in Loeb Quad)

5:00: – 5:15: Course Photo #1; wear course t-shirt (Loeb Courtyard)

6:00: – 10:00: Course Dinner and Student Awards with Course Photo #2 at 6:30: pm (Swope Upper Terrace)

Thurs 10 Aug

Breakfast, room checkout and departure