Zebrafish Schedule
Please click here to download the 2022 Zebrafish Course Schedule.
Thurs 28 Jul
2:00 – 5:00 |
Student check-in |
6:00 – 6:30 |
Light dinner – cold cuts & veggies |
6:30 – 6:45 |
Welcome: Tom Schilling and Debbie Yelon |
6:45 – 7:45 |
Overview: Zebrafish Development and Genetics: Lila Solnica-Krezel |
7:45 – 9:30 |
Student 3-min oral presentations |
9:30 – 10:00 |
Set up posters and locate individual student stations in lab |
Fri 29 Jul
8:30 – 9:15 |
Introduction to stereoscopes, iPad/camera & server: Dave Raible and faculty |
9:15 – noon |
Pit Talk & Lab: Zebrafish development (temporal dynamics, tissue organization, early embryo anatomy): Lila Solnica-Krezel, Dave Raible |
noon – 1:30 |
Faculty (Lila Solnica-Krezel and Dave Raible) lunch with students |
1:30 – 1:45 |
Check nocodazole-treated embryos from morning session: Solnica-Krezel |
1:45 – 6:00 |
Pit Talk & Lab: Adult Dissection and Regeneration (fin and heart): Ken Poss |
7:00 – 7:45 |
Research Lecture & Discussion: Dave Raible |
8:00 – 9:00 |
MBL Friday Evening Lecture |
9:00 – 9:30 |
Check SB-treated embryos and wrap-up from morning session: Solnica-Krezel |
9:30 – 11:30 |
Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 1) |
9:30 – 11:30 |
Overnight time-lapse microscopy: Jan Huisken (Group 4) |
9:30 – 11:00 |
Poster Perusal: Groups 2 & 3 |
Sat 30 Jul
9:00 – 10:00 |
Pit Talk: Larval anatomy: Tom Schilling |
10:00 – 11:00 |
Lecture: Introduction to Microscopy: Andres Collazo |
11:00 – noon |
Hands-on microscopy and larval anatomy – fluorescent dissecting scopes: Andres Collazo, Jim Fadool, Dave Raible, Tom Schilling, Debbie Yelon |
noon – 1:30 |
Faculty (Andres Collazo and Ken Poss) lunch with students |
1:30 – 3:00 |
Hands-on microscopy and larval anatomy – compound scopes: Collazo, Fadool, Raible, Schilling, Yelon |
3:00 – 4:00 |
Student groups write up customized protocols for their scopes |
4:00 – 6:00 |
Lecture: Light Sheet Microscopy: Jan Huisken |
7:30 – 9:00 |
Research Lectures & Discussion: Alex Schier and Ken Poss |
9:00 – 11:00 |
Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 2) |
9:00 – 11:00 |
Overnight time-lapse microscopy: Jan Huisken (Group 3) |
9:00 – 11:00 |
Poster Perusal: Groups 1 & 4 |
Sat July 31
8:30 – noon |
Pit Talk & Lab: mRNA and CRISPR injections: Andi Pauli and Becky Burdine |
noon – 1:30 |
Faculty (Alex Schier and Jan Huisken) lunch with students |
1:30 – 2:30 |
Developmental Genetics review: Mary Mullins |
2:30 – 6:00 |
Zebrafish genome literacy workshop: Elisabeth Busch-Nentwich |
7:30 – 9:00 |
Research Lectures & Discussion: Becky Burdine and Lila Solnica-Krezel |
9:00 – 9:30 |
Wrap-up mRNA injected embryos: Andi Pauli and Becky Burdine |
9:30 – 11:30 |
Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 3) |
9:30 – 11:30 |
Overnight time-lapse microscopy: Jan Huisken (Group 1) |
9:30 – 11:00 |
Poster Perusal: Groups 2 & 4 |
Mon 1 Aug
8:30 – noon |
Pit Talk & Lab: Morpholino & CRISPR injections: Andi Pauli and Becky Burdine |
noon – 1:30 |
Faculty (Becky Burdine and Mary Mullins) lunch with students |
1:30 – 2:30 |
Roundtable: Morpholino use: Mary Mullins and additional faculty |
2:30 – 5:00 |
CRISPR design workshop: Antonio Giraldez |
5:00 – 6:00 |
Roundtable: When should I (not) use zebrafish as a model system?: Alex Schier |
7:30 – 9:00 |
Research Lectures & Discussion: Mary Mullins and Elisabeth Busch-Nentwich |
9:30 – 11:30 |
Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 4) |
9:30 – 11:30 |
Overnight time-lapse microscopy: Jan Huisken (Group 2) |
9:30 – 11:00 |
Poster Perusal: Groups 1 & 3 |
Tues 2 Aug
8:30 – 9:00 |
Introduction to ZFIN, atlases, and web resources: Corinne Houart and Sharon Amacher |
9:00 – 9:30 |
Pit Talk: Immunostaining & in situ hybridization: Houart and Amacher |
9:30 – noon |
Mounting, analysis, and imaging of fixed embryos: Houart and Amacher |
noon – 1:30 |
Faculty (Andi Pauli and Elisabeth Busch-Nentwich) lunch with students |
1:30 – 4:30 |
Lab: Continuation from morning and make presentations of resulting images |
4:30 – 6:00 |
Research Lectures & Discussion: Andi Pauli and Sharon Amacher |
7:30 – 10:00 |
Faculty & Staff Event; Student self-organized social time |
Wed 3 Aug
9:00 – 10:00 |
Roundtable: Zebrafish in Education: Roshan Jain, Pierre LePabic, Sharon Amacher and faculty |
10:00 – noon |
Wrap-up: Immunostaining & in situ hybridization: student presentations with Houart and Amacher |
noon – 1:00 |
Faculty (Sharon Amacher and Antonio Giraldez) lunch with students |
1:00 – 3:00 |
Check on regenerating fins: Poss and Yelon |
3:00 – 5:00 |
Free Time (Faculty meeting held for 1 hour during this time) |
5:00 – 6:00 |
Student/Faculty Challenge |
6:30 – 9:00 |
Course BBQ |
Thurs 4 Aug
9:00 – 10:30 |
Roundtable: Responsible Conduct in Research: Schilling and Yelon 10:30 – noon Pit Talk: Chimeric Analysis: Sharon Amacher and Jim Fadool noon – 1:00 Faculty (Corinne Houart and Debbie Yelon) lunch with students |
1:00 – 6:00 |
Lab: Chimeric analysis – Blastula transplants (including germ cell transplants): Sharon Amacher, Antonio Giraldez, Jim Fadool, Andres Collazo, and Tom Schilling |
7:30 – 9:00 |
Research Lectures & Discussion: Corinne Houart and Antonio Giraldez |
9:00 – 11:00 |
Optional overnight time-lapse microscopy of transplants (on your own) |
Fri 5 Aug
9:00 – 9:30 |
Pit Talk: “High resolution” transplants: Corinne Houart |
9:30 – noon |
Lab: “High resolution” transplants (Houart; small groups rotate) and imaging early transplants (Giraldez, Fadool, Collazo, and Schilling) |
Noon – 1:30 |
Faculty (Anna Huttenlocher and Jim Fadool) lunch with students |
1:30 – 4:00 |
Continuation of transplant lab (Houart) AND Check on regenerating fins (Poss and Yelon) |
4:00 – 5:00 |
Roundtable: Disease modeling in zebrafish: Anna Huttenlocher |
7:00 – 7:45 |
Research Lecture & Discussion: Anna Huttenlocher |
8:00 – 9:00 |
MBL Friday Evening Lecture |
9:00 – 11:00 |
Additional imaging of transplants; assembly of images for transplant wrap-up |
Sat 6 Aug
9:00 – 11:00 |
Wrap-up: Chimeric analysis: student presentations with Giraldez, Fadool, Collazo, and Schilling |
11:00 – noon |
Pit Talk: Imaging & Quantification of cell signaling in vivo: Anna Huttenlocher noon – 1:30 Faculty (Isaac Bianco and Mark Masino) lunch with students |
1:30 – 6:00 |
Lab: Imaging & Quantification of cell signaling in vivo: Anna Huttenlocher |
7:30 – 9:00 |
Research Lectures & Discussion: Isaac Bianco and Mark Masino |
9:00 – 11:00 |
Fin regeneration results and terminal imaging: Poss and Yelon |
Sun 7 Aug
9:00 – 10:00 |
Pit Talk: Behavior: Isaac Bianco, Jim Fadool, and Mark Masino |
10:00 – noon |
Lab: Behavior: Bianco, Fadool, and Masino |
noon – 1:30 |
Faculty (Sarah Kucenas and David Prober) lunch with students |
1:30 – 3:30 |
Lab, continued: Behavior: Bianco, Fadool, and Masino |
4:00 – 5:00 |
Wrap-up of Imaging & Quantification: Anna Huttenlocher |
5:00 – 5:45 |
Research Lecture & Discussion: Jim Fadool |
7:30 – 9:00 |
Chi-Bin Chien Memorial Research Lecture: David Prober |
Mon 8 Aug
9:00 – 11:00 |
MRC tours |
11:00 – 2:00 |
Fish facility visit & Lunch with vendors: Aquatic Systems: Schilling, Yelon, & vendors |
2:00 – 4:00 |
Lecture and Discussion: Husbandry, breeding and raising fry: April Freeman |
4:30 – 6:00 |
Roundtable: Transgenesis: Sarah Kucenas |
7:30 – 9:00 |
Research Lectures & Discussion: Sarah Kucenas and Debbie Yelon |
10:00 |
Stony Beach evening quiet field trip to see bioluminescence |
Tues 9 Aug
9:00 – 10:00 |
Pit Talk: Optical Manipulation: Sarah Kucenas and Debbie Yelon |
10:00 – noon |
Lab Rotations: Photoconversion: Yelon, Ablation/Axotomy: Kucenas AND work on photo contest submissions and 5-minute student presentations of favorite data |
noon – 1:30 |
Faculty (Roshan Jain and Pierre Le Pabic) lunch with students |
1:30 – 3:30 |
Lab Rotations: Photoconversion: Yelon, Ablation/Axotomy: Kucenas AND work on photo contest submissions and 5-minute student presentations of favorite data |
3:30 – 6:00 |
Cardiac regeneration results and terminal imaging: Poss and Yelon |
7:30 – 9:00 |
Research Lectures & Discussion: Clare Buckley and Tom Schilling |
9:00 – 11:00 |
Finish up photo contest submissions and 5-minute student presentations of favorite data |
11:00 |
Photo contest images (individual & group entries) due by 11:00 pm |
Wed 10 Aug
8:30 – 9:00 |
Pit Talk: Optogenetic Manipulation: Clare Buckley |
9:00 – noon |
Lab: Optogenetic Manipulation: Clare Buckley AND finalize preparation of 5-min presentations AND clean-up stations/return materials/download data and documents |
noon – 1:30 |
Faculty (Clare Buckley and Tom Schilling) lunch with students |
1:30 – 3:30 |
Five-minute student presentations of favorite piece of data |
3:30 – 5:00 |
Course Wrap-up: discussion with Schilling and Yelon |
5:00 – 5:15 |
Course Photo #1; wear course t-shirt |
6:00 – 10:00 |
Course Dinner and Student Awards with Course Photo #2 at 6:30 pm |
Thurs 11 Aug
Breakfast, room checkout and departure