2024 Zebrafish Schedule
Thurs 25 Jul
2:00 – 5:00: Student check-in
6:00 – 7:00: Light dinner – cold cuts & veggies
7:00 – 7:15: Welcome to the Zebrafish Course: Tom Schilling and Debbie Yelon
7:15 – 8:15: Overview: Zebrafish Development and Genetics: Lila Solnica-Krezel
8:15 – 9:00: Student 3-min oral presentations
9:00 – 10:00: Set up posters and locate individual student stations in lab
Fri 26 Jul
8:30 – 8:45: Welcome to the MBL: Linda Hyman, Director of Education
8:45 – 9:15: Introduction to stereoscopes, iPad/camera & server: Dave Raible and faculty
9:15 – 12:00: Pit Talk & Lab: Zebrafish development (temporal dynamics, tissue organization, early embryo anatomy): Lila Solnica-Krezel, Dave Raible
12:00 – 1:30: Faculty (Lila Solnica-Krezel and Dave Raible) lunch with students
1:30 – 6:00: Pit Talk & Lab: Adult Dissection and Regeneration (fin and heart): Ken Poss
7:30 – 9:00: Research Lectures & Discussion: Dave Raible and Lila Solnica-Krezel
9:00 – 9:30: Follow up on embryos and wrap-up from morning session: Solnica-Krezel
9:30 – 11:00: Poster Session
Sat 27 Jul
8:30 – 9:30: Lecture: Introduction to Microscopy: Andres Collazo
9:30 – 12:00: Hands-on microscopy – practice Kohler illumination, learn Zen software: Andres Collazo, Jim Fadool, Dave Raible, Tom Schilling
12:00 – 1:30: Faculty (Tom Schilling and Ken Poss) lunch with students
1:30 – 2:30: Pit Talk: Larval anatomy: Tom Schilling
2:30 – 5:00: Larval anatomy – touring larvae with fluorescent transgenics: Collazo, Fadool, Raible, Schilling
5:00 – 6:00: Lecture: Light Sheet Microscopy: Jan Huisken
7:30 – 9:00: Research Lectures & Discussion: Andi Pauli and Ken Poss
9:30 – 11:00: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 1)
9:30 – 11:00: Overnight time-lapse microscopy: Jan Huisken (Group 4)
Sun 28 Jul
9:00 – 10:30: Workshop: Developmental Genetics: Mary Mullins
10:30 – 12:00: Workshop: Zebrafish in Education: Roshan Jain, Pierre Le Pabic, and Kara Cerveny
12:00 – 1:30: Faculty (Andi Pauli and Andres Collazo) lunch with students
1:30 – 3:00: Workshop: Testing Gene Function and Designer Genes: Mary Mullins
3:30 – 6:00: Workshop: Responsible Conduct in Research: Schilling and Yelon
7:30 – 9:00: Research Lectures & Discussion: Becky Burdine and Mary Mullins
9:30 – 11:00: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 2)
9:30 – 11:00: Overnight time-lapse microscopy: Jan Huisken (Group 3)
Mon 29 Jul
8:30 – 12:00: Pit Talk & Lab: mRNA and CRISPR injections: Andi Pauli and Becky Burdine
12:00 – 1:30: Faculty (Jan Huisken and Mary Mullins) lunch with students
1:30 – 3:00: Review first set of time-lapses (Groups 1, 2, 3, 4): Fadool, Collazo, Huisken
3:00 – 6:00: Workshop: Zebrafish Genome Literacy and CRISPR/Cas9 Experimental Design: Maura McGrail
7:30 – 9:00: Research Lectures & Discussion: Maura McGrail and Kara Cerveny
9:00 – 9:30: Wrap-up mRNA injected embryos: Andi Pauli and Becky Burdine
9:30 – 11:00: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 4)
9:30 – 11:00: Overnight time-lapse microscopy: Jan Huisken (Group 1)
Tues 30 Jul
9:00 – 12:00: Pit Talk & Lab: Morpholino & CRISPR injections: Andi Pauli and Becky Burdine
11:00 – 12:00: Needle pulling demo: Adair Oesterle (Sutter Instruments)
12:00 – 1:30: Faculty (Maura McGrail and Becky Burdine) lunch with students
1:30 – 5:30: Free Time!
1:30 – 2:30: Faculty Meeting
5:30 – 6:30: Student/Faculty Challenge
6:30 – 9:00: Course BBQ
9:00 – 10:00: Check on mRNA injected embryos: Andi Pauli and Becky Burdine
Wed 31 Jul
8:30 – 9:00: Introduction to ZFIN, atlases, and web resources: Corinne Houart and Kara Cerveny
9:00 – 9:30: Pit Talk: Immunostaining & in situ hybridization: Houart and Cerveny
9:30 – 12:00: Mounting, analysis, and imaging of fixed embryos: Houart and Cerveny
12:00 – 1:30: Faculty (Kara Cerveny and Holger Knaut) lunch with students
1:30 – 6:00: Lab: Continuation from morning and make presentations of resulting images
7:00 – 10:00: Faculty & Staff Event; Student self-organized social time and/or assembly of images for next day's wrap-up
Thurs 1 Aug
8:30 – 10:30: Wrap-up: Immunostaining & in situ hybridization: student presentations with Houart and Cerveny
10:30 – 12:00: Pit Talk: Chimeric Analysis: Jim Fadool and Holger Knaut
12:00 – 1:30: Faculty (Corinne Houart and Debbie Yelon) lunch with students
1:30 – 6:00: Lab: Chimeric analysis – Blastula transplants: Andreas Collazo, Jim Fadool, Holger Knaut, and Tom Schilling
4:30 – 6:00: Possible early start for some students on late transplants: Corinne Houart
7:30 – 9:00: Research Lectures & Discussion: Corinne Houart and Holger Knaut
9:30 – 11:00: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 3)
9:30 – 11:00: Overnight time-lapse microscopy: Jan Huisken (Group 2)
Fri 2 Aug
8:30 – 9:30: Pit Talk: “High resolution” transplants: Corinne Houart
9:30 – 12:00: Lab: “High resolution” transplants (Houart; small groups rotate) and imaging early transplants (Collazo, Fadool, Knaut, and Schilling)
12:00 – 1:30: Faculty (Anna Huttenlocher and Jim Fadool) lunch with students
1:30 – 6:00: Continuation of transplant lab (Houart) and imaging early transplants (Collazo, Fadool, Knaut, and Schilling)
7:30 – 9:00: Research Lectures & Discussion: Anna Huttenlocher and Kristen Kwan
9:30 – 11:00: Additional imaging of transplants; assembly of images for transplant wrap-up
Sat 3 Aug
8:30 – 10:30: Wrap-up: Chimeric analysis: student presentations with Collazo, Houart, Fadool, Knaut, and Schilling
10:30 – 12:00: Pit Talk: Tracking migratory cells in vivo: Anna Huttenlocher
12:00 – 1:3: Faculty (Harry Burgess and Kristen Kwan) lunch with students
1:30 – 6:00: Lab: Tracking migratory cells in vivo: Anna Huttenlocher
7:30 – 9:00: Research Lectures & Discussion: Harry Burgess and Jim Fadool
9:30 – 11:00: Fin regeneration results and terminal imaging: Poss and Yelon
Sun 4 Aug
8:30 – 9:30: Wrap-up of tracking migratory cells: Anna Huttenlocher
9:30 – 10:30: Workshop: Disease modeling in zebrafish: Anna Huttenlocher
10:30 – 12:00: Pit Talk: Monitoring morphogenesis: Analysis of morphogenesis in 3D and 4D: Kristen Kwan
12:00 – 1:30: Faculty (Roshan Jain and Pierre Le Pabic) lunch with students
1:30 – 5:00: Lab: Monitoring morphogenesis: Kristen Kwan
5:00 – 6:00: Workshop: Single-cell and Spatial Transcriptomics in Zebrafish: Alex Schier
7:30 – 9:00: Chi-Bin Chien Memorial Research Lecture: David Schoppik, followed by reception
Mon 5 Aug
8:30 – 9:30: Wrap-up of monitoring morphogenesis: Kristen Kwan
9:30 – 10:30: Pit Talk: Behavior: Harry Burgess and Jim Fadool
10:30 – 12:30: Lab: Behavior: Harry Burgess and Jim Fadool
12:30 – 2:00: Faculty (David Schoppik) lunch with students
2:00 – 5:00: Lab, continued: Behavior: Burgess and Fadool
5:00 – 6:00: Workshop: Transgenesis: Sarah Kucenas
7:30 – 9:00: Research Lectures & Discussion: Sarah Kucenas and Alex Schier
Tues 6 Aug
8:30 – 9:30: Pit Talk: Optical Manipulation: Sarah Kucenas and Debbie Yelon
9:30 – 12:30: Lab Rotations: Photoconversion: Yelon, Ablation/Axotomy: Kucenas
12:30 – 1:30: Lunch
1:30 – 3:45: Lab Rotations: Ablation/Axotomy: Kucenas AND Fish facility tours with aquaculture vendors
3:45 – 4:15: Tea time with aquaculture vendors
4:15 – 6:00: Workshop: Zebrafish Husbandry and Breeding: April Freeman
7:30 – 9:00: Research Lectures & Discussion: Debbie Yelon and Tom Schilling
9:30 – 11:00: Cardiac regeneration results and terminal imaging: Poss and Yelon
Wed 7 Aug
8:30 – 11:00: In vitro fertilization: April Freeman and Debbie Yelon (Loeb 260) AND Follow up on Ablation/Axotomy or Photoconversion: Kucenas and Yelon
11:00 – 12:00: Workshop: When should I (not) use zebrafish as a model system?: Alex Schier
12:00 – 1:30: Faculty (Alex Schier and Sarah Kucenas) lunch with students
1:30 – 3:30: Five-minute student presentations of favorite data
3:30 – 5:00: Course Wrap-up: discussion with Schilling and Yelon
5:00 – 5:15: Course Photo #1; wear course t-shirt
6:00 – 10:00: Course Dinner and Student Awards with Course Photo #2 at 6:30 pm
Thurs 8 Aug
Breakfast, room checkout and departure