Zebrafish Schedule

2:00 – 5:00

Student check-in

6:00 – 6:30

Light dinner – cold cuts & veggies

6:30 – 6:45

Welcome: Tom Schilling and Debbie Yelon

6:45 – 7:45

Overview: Zebrafish Development and Genetics: Lila Solnica-Krezel

7:45 – 9:30

Student 3-min oral presentations

9:30 – 10:00

Set up posters and locate individual student stations in lab

8:30 – 9:15

Introduction to stereoscopes, iPad/camera & server: Dave Raible and faculty

9:15 – noon

Pit Talk & Lab: Zebrafish development (temporal dynamics, tissue organization, early embryo anatomy): Lila Solnica-Krezel, Dave Raible

noon – 1:30

Faculty (Lila Solnica-Krezel and Dave Raible) lunch with students

1:30 – 1:45

Check nocodazole-treated embryos from morning session: Solnica-Krezel

1:45 – 6:00

Pit Talk & Lab: Adult Dissection and Regeneration (fin and heart): Ken Poss

7:00 – 7:45

Research Lecture & Discussion: Dave Raible

8:00 – 9:00

MBL Friday Evening Lecture

9:00 – 9:30

Check SB-treated embryos and wrap-up from morning session: Solnica-Krezel

9:30 – 11:30

Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 1)

9:30 – 11:30

Overnight time-lapse microscopy: Jan Huisken (Group 4)

9:30 – 11:00

Poster Perusal: Groups 2 & 3

9:00 – 10:00

Pit Talk: Larval anatomy: Tom Schilling

10:00 – 11:00

Lecture: Introduction to Microscopy: Andres Collazo

11:00 – noon

Hands-on microscopy and larval anatomy – fluorescent dissecting scopes: Andres Collazo, Jim Fadool, Dave Raible, Tom Schilling, Debbie Yelon

noon – 1:30

Faculty (Andres Collazo and Ken Poss) lunch with students

1:30 – 3:00

Hands-on microscopy and larval anatomy – compound scopes: Collazo, Fadool, Raible, Schilling, Yelon

3:00 – 4:00

Student groups write up customized protocols for their scopes

4:00 – 6:00

Lecture: Light Sheet Microscopy: Jan Huisken

7:30 – 9:00

Research Lectures & Discussion: Alex Schier and Ken Poss

9:00 – 11:00

Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 2)

9:00 – 11:00

Overnight time-lapse microscopy: Jan Huisken (Group 3)

9:00 – 11:00

Poster Perusal: Groups 1 & 4

8:30 – noon

Pit Talk & Lab: mRNA and CRISPR injections: Andi Pauli and Becky Burdine

noon – 1:30

Faculty (Alex Schier and Jan Huisken) lunch with students

1:30 – 2:30

Developmental Genetics review: Mary Mullins

2:30 – 6:00

Zebrafish genome literacy workshop: Elisabeth Busch-Nentwich

7:30 – 9:00

Research Lectures & Discussion: Becky Burdine and Lila Solnica-Krezel

9:00 – 9:30

Wrap-up mRNA injected embryos: Andi Pauli and Becky Burdine

9:30 – 11:30

Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 3)

9:30 – 11:30

Overnight time-lapse microscopy: Jan Huisken (Group 1)

9:30 – 11:00

Poster Perusal: Groups 2 & 4

8:30 – noon

Pit Talk & Lab: Morpholino & CRISPR injections: Andi Pauli and Becky Burdine

noon – 1:30

Faculty (Becky Burdine and Mary Mullins) lunch with students

1:30 – 2:30

Roundtable: Morpholino use: Mary Mullins and additional faculty

2:30 – 5:00

CRISPR design workshop: Antonio Giraldez

5:00 – 6:00

Roundtable: When should I (not) use zebrafish as a model system?: Alex Schier

7:30 – 9:00

Research Lectures & Discussion: Mary Mullins and Elisabeth Busch-Nentwich

9:30 – 11:30

Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 4)

9:30 – 11:30

Overnight time-lapse microscopy: Jan Huisken (Group 2)

9:30 – 11:00

Poster Perusal: Groups 1 & 3

8:30 – 9:00

Introduction to ZFIN, atlases, and web resources: Corinne Houart and Sharon Amacher

9:00 – 9:30

Pit Talk: Immunostaining & in situ hybridization: Houart and Amacher

9:30 – noon

Mounting, analysis, and imaging of fixed embryos: Houart and Amacher

noon – 1:30

Faculty (Andi Pauli and Elisabeth Busch-Nentwich) lunch with students

1:30 – 4:30

Lab: Continuation from morning and make presentations of resulting images

4:30 – 6:00

Research Lectures & Discussion: Andi Pauli and Sharon Amacher

7:30 – 10:00

Faculty & Staff Event; Student self-organized social time

9:00 – 10:00

Roundtable: Zebrafish in Education: Roshan Jain, Pierre LePabic, Sharon Amacher and faculty

10:00 – noon

Wrap-up: Immunostaining & in situ hybridization: student presentations with Houart and Amacher

noon – 1:00

Faculty (Sharon Amacher and Antonio Giraldez) lunch with students

1:00 – 3:00

Check on regenerating fins: Poss and Yelon

3:00 – 5:00

Free Time (Faculty meeting held for 1 hour during this time)

5:00 – 6:00

Student/Faculty Challenge

6:30 – 9:00

Course BBQ

9:00 – 10:30

Roundtable: Responsible Conduct in Research: Schilling and Yelon 10:30 – noon Pit Talk: Chimeric Analysis: Sharon Amacher and Jim Fadool noon – 1:00 Faculty (Corinne Houart and Debbie Yelon) lunch with students

1:00 – 6:00

Lab: Chimeric analysis – Blastula transplants (including germ cell transplants): Sharon Amacher, Antonio Giraldez, Jim Fadool, Andres Collazo, and Tom Schilling

7:30 – 9:00

Research Lectures & Discussion: Corinne Houart and Antonio Giraldez

9:00 – 11:00

Optional overnight time-lapse microscopy of transplants (on your own)

9:00 – 9:30

Pit Talk: “High resolution” transplants: Corinne Houart

9:30 – noon

Lab: “High resolution” transplants (Houart; small groups rotate) and imaging early transplants (Giraldez, Fadool, Collazo, and Schilling)

Noon – 1:30

Faculty (Anna Huttenlocher and Jim Fadool) lunch with students

1:30 – 4:00

Continuation of transplant lab (Houart) AND Check on regenerating fins (Poss and Yelon)

4:00 – 5:00

Roundtable: Disease modeling in zebrafish: Anna Huttenlocher

7:00 – 7:45

Research Lecture & Discussion: Anna Huttenlocher

8:00 – 9:00

MBL Friday Evening Lecture

9:00 – 11:00

Additional imaging of transplants; assembly of images for transplant wrap-up

9:00 – 11:00

Wrap-up: Chimeric analysis: student presentations with Giraldez, Fadool, Collazo, and Schilling

11:00 – noon

Pit Talk: Imaging & Quantification of cell signaling in vivo: Anna Huttenlocher noon – 1:30 Faculty (Isaac Bianco and Mark Masino) lunch with students

1:30 – 6:00

Lab: Imaging & Quantification of cell signaling in vivo: Anna Huttenlocher

7:30 – 9:00

Research Lectures & Discussion: Isaac Bianco and Mark Masino

9:00 – 11:00

Fin regeneration results and terminal imaging: Poss and Yelon

9:00 – 10:00

Pit Talk: Behavior: Isaac Bianco, Jim Fadool, and Mark Masino

10:00 – noon

Lab: Behavior: Bianco, Fadool, and Masino

noon – 1:30

Faculty (Sarah Kucenas and David Prober) lunch with students

1:30 – 3:30

Lab, continued: Behavior: Bianco, Fadool, and Masino

4:00 – 5:00

Wrap-up of Imaging & Quantification: Anna Huttenlocher

5:00 – 5:45

Research Lecture & Discussion: Jim Fadool

7:30 – 9:00

Chi-Bin Chien Memorial Research Lecture: David Prober

9:00 – 11:00

MRC tours

11:00 – 2:00

Fish facility visit & Lunch with vendors: Aquatic Systems: Schilling, Yelon, & vendors

2:00 – 4:00

Lecture and Discussion: Husbandry, breeding and raising fry: April Freeman

4:30 – 6:00

Roundtable: Transgenesis: Sarah Kucenas

7:30 – 9:00

Research Lectures & Discussion: Sarah Kucenas and Debbie Yelon

10:00

Stony Beach evening quiet field trip to see bioluminescence

9:00 – 10:00

Pit Talk: Optical Manipulation: Sarah Kucenas and Debbie Yelon

10:00 – noon

Lab Rotations: Photoconversion: Yelon, Ablation/Axotomy: Kucenas AND work on photo contest submissions and 5-minute student presentations of favorite data

noon – 1:30

Faculty (Roshan Jain and Pierre Le Pabic) lunch with students

1:30 – 3:30

Lab Rotations: Photoconversion: Yelon, Ablation/Axotomy: Kucenas AND work on photo contest submissions and 5-minute student presentations of favorite data

3:30 – 6:00

Cardiac regeneration results and terminal imaging: Poss and Yelon

7:30 – 9:00

Research Lectures & Discussion: Clare Buckley and Tom Schilling

9:00 – 11:00

Finish up photo contest submissions and 5-minute student presentations of favorite data

11:00

Photo contest images (individual & group entries) due by 11:00 pm

8:30 – 9:00

Pit Talk: Optogenetic Manipulation: Clare Buckley

9:00 – noon

Lab: Optogenetic Manipulation: Clare Buckley AND finalize preparation of 5-min presentations AND clean-up stations/return materials/download data and documents

noon – 1:30

Faculty (Clare Buckley and Tom Schilling) lunch with students

1:30 – 3:30

Five-minute student presentations of favorite piece of data

3:30 – 5:00

Course Wrap-up: discussion with Schilling and Yelon

5:00 – 5:15

Course Photo #1; wear course t-shirt

6:00 – 10:00

Course Dinner and Student Awards with Course Photo #2 at 6:30 pm