2024 Zebrafish Schedule

Thurs 25 Jul

2:00 – 5:00: Student check-in

6:00 – 7:00: Light dinner – cold cuts & veggies

7:00 – 7:15: Welcome to the Zebrafish Course: Tom Schilling and Debbie Yelon

7:15 – 8:15: Overview: Zebrafish Development and Genetics: Lila Solnica-Krezel

8:15 – 9:00: Student 3-min oral presentations

9:00 – 10:00: Set up posters and locate individual student stations in lab

Fri 26 Jul

8:30 – 8:45: Welcome to the MBL: Linda Hyman, Director of Education

8:45 – 9:15: Introduction to stereoscopes, iPad/camera & server: Dave Raible and faculty

9:15 – 12:00: Pit Talk & Lab: Zebrafish development (temporal dynamics, tissue organization, early embryo anatomy): Lila Solnica-Krezel, Dave Raible

12:00 – 1:30: Faculty (Lila Solnica-Krezel and Dave Raible) lunch with students

1:30 – 6:00: Pit Talk & Lab: Adult Dissection and Regeneration (fin and heart): Ken Poss

7:30 – 9:00: Research Lectures & Discussion: Dave Raible and Lila Solnica-Krezel

9:00 – 9:30: Follow up on embryos and wrap-up from morning session: Solnica-Krezel

9:30 – 11:00: Poster Session

Sat 27 Jul

8:30 – 9:30: Lecture: Introduction to Microscopy: Andres Collazo

9:30 – 12:00: Hands-on microscopy – practice Kohler illumination, learn Zen software: Andres Collazo, Jim Fadool, Dave Raible, Tom Schilling

12:00 – 1:30: Faculty (Tom Schilling and Ken Poss) lunch with students

1:30 – 2:30: Pit Talk: Larval anatomy: Tom Schilling

2:30 – 5:00: Larval anatomy – touring larvae with fluorescent transgenics: Collazo, Fadool, Raible, Schilling

5:00 – 6:00: Lecture: Light Sheet Microscopy: Jan Huisken

7:30 – 9:00: Research Lectures & Discussion: Andi Pauli and Ken Poss

9:30 – 11:00: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 1)

9:30 – 11:00: Overnight time-lapse microscopy: Jan Huisken (Group 4)

Sun 28 Jul

9:00 – 10:30: Workshop: Developmental Genetics: Mary Mullins

10:30 – 12:00: Workshop: Zebrafish in Education: Roshan Jain, Pierre Le Pabic, and Kara Cerveny

12:00 – 1:30: Faculty (Andi Pauli and Andres Collazo) lunch with students

1:30 – 3:00: Workshop: Testing Gene Function and Designer Genes: Mary Mullins

3:30 – 6:00: Workshop: Responsible Conduct in Research: Schilling and Yelon

7:30 – 9:00: Research Lectures & Discussion: Becky Burdine and Mary Mullins

9:30 – 11:00: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 2)

9:30 – 11:00: Overnight time-lapse microscopy: Jan Huisken (Group 3)

Mon 29 Jul

8:30 – 12:00: Pit Talk & Lab: mRNA and CRISPR injections: Andi Pauli and Becky Burdine

12:00 – 1:30: Faculty (Jan Huisken and Mary Mullins) lunch with students

1:30 – 3:00: Review first set of time-lapses (Groups 1, 2, 3, 4): Fadool, Collazo, Huisken

3:00 – 6:00: Workshop: Zebrafish Genome Literacy and CRISPR/Cas9 Experimental Design: Maura McGrail

7:30 – 9:00: Research Lectures & Discussion: Maura McGrail and Kara Cerveny

9:00 – 9:30: Wrap-up mRNA injected embryos: Andi Pauli and Becky Burdine

9:30 – 11:00: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 4)

9:30 – 11:00: Overnight time-lapse microscopy: Jan Huisken (Group 1)

Tues 30 Jul

9:00 – 12:00: Pit Talk & Lab: Morpholino & CRISPR injections: Andi Pauli and Becky Burdine

11:00 – 12:00: Needle pulling demo: Adair Oesterle (Sutter Instruments)

12:00 – 1:30: Faculty (Maura McGrail and Becky Burdine) lunch with students

1:30 – 5:30: Free Time!

1:30 – 2:30: Faculty Meeting

5:30 – 6:30: Student/Faculty Challenge

6:30 – 9:00: Course BBQ

9:00 – 10:00: Check on mRNA injected embryos: Andi Pauli and Becky Burdine

Wed 31 Jul

8:30 – 9:00: Introduction to ZFIN, atlases, and web resources: Corinne Houart and Kara Cerveny

9:00 – 9:30: Pit Talk: Immunostaining & in situ hybridization: Houart and Cerveny

9:30 – 12:00: Mounting, analysis, and imaging of fixed embryos: Houart and Cerveny

12:00 – 1:30: Faculty (Kara Cerveny and Holger Knaut) lunch with students

1:30 – 6:00: Lab: Continuation from morning and make presentations of resulting images

7:00 – 10:00: Faculty & Staff Event; Student self-organized social time and/or assembly of images for next day's wrap-up

Thurs 1 Aug

8:30 – 10:30: Wrap-up: Immunostaining & in situ hybridization: student presentations with Houart and Cerveny

10:30 – 12:00: Pit Talk: Chimeric Analysis: Jim Fadool and Holger Knaut

12:00 – 1:30: Faculty (Corinne Houart and Debbie Yelon) lunch with students

1:30 – 6:00: Lab: Chimeric analysis – Blastula transplants: Andreas Collazo, Jim Fadool, Holger Knaut, and Tom Schilling

4:30 – 6:00: Possible early start for some students on late transplants: Corinne Houart

7:30 – 9:00: Research Lectures & Discussion: Corinne Houart and Holger Knaut

9:30 – 11:00: Overnight time-lapse microscopy: Andres Collazo and Jim Fadool (Group 3)

9:30 – 11:00: Overnight time-lapse microscopy: Jan Huisken (Group 2)

Fri 2 Aug

8:30 – 9:30: Pit Talk: “High resolution” transplants: Corinne Houart

9:30 – 12:00: Lab: “High resolution” transplants (Houart; small groups rotate) and imaging early transplants (Collazo, Fadool, Knaut, and Schilling)

12:00 – 1:30: Faculty (Anna Huttenlocher and Jim Fadool) lunch with students

1:30 – 6:00: Continuation of transplant lab (Houart) and imaging early transplants (Collazo, Fadool, Knaut, and Schilling)

7:30 – 9:00: Research Lectures & Discussion: Anna Huttenlocher and Kristen Kwan

9:30 – 11:00: Additional imaging of transplants; assembly of images for transplant wrap-up

Sat 3 Aug

8:30 – 10:30: Wrap-up: Chimeric analysis: student presentations with Collazo, Houart, Fadool, Knaut, and Schilling

10:30 – 12:00: Pit Talk: Tracking migratory cells in vivo: Anna Huttenlocher

12:00 – 1:3: Faculty (Harry Burgess and Kristen Kwan) lunch with students

1:30 – 6:00: Lab: Tracking migratory cells in vivo: Anna Huttenlocher

7:30 – 9:00: Research Lectures & Discussion: Harry Burgess and Jim Fadool

9:30 – 11:00: Fin regeneration results and terminal imaging: Poss and Yelon

Sun 4 Aug

8:30 – 9:30: Wrap-up of tracking migratory cells: Anna Huttenlocher

9:30 – 10:30: Workshop: Disease modeling in zebrafish: Anna Huttenlocher

10:30 – 12:00: Pit Talk: Monitoring morphogenesis: Analysis of morphogenesis in 3D and 4D: Kristen Kwan

12:00 – 1:30: Faculty (Roshan Jain and Pierre Le Pabic) lunch with students  

1:30 – 5:00: Lab: Monitoring morphogenesis: Kristen Kwan

5:00 – 6:00: Workshop: Single-cell and Spatial Transcriptomics in Zebrafish: Alex Schier

7:30 – 9:00: Chi-Bin Chien Memorial Research Lecture: David Schoppik, followed by reception

Mon 5 Aug

8:30 – 9:30: Wrap-up of monitoring morphogenesis: Kristen Kwan

9:30 – 10:30: Pit Talk: Behavior: Harry Burgess and Jim Fadool

10:30 – 12:30: Lab: Behavior: Harry Burgess and Jim Fadool

12:30 – 2:00: Faculty (David Schoppik) lunch with students

2:00 – 5:00: Lab, continued: Behavior: Burgess and Fadool

5:00 – 6:00: Workshop: Transgenesis: Sarah Kucenas

7:30 – 9:00: Research Lectures & Discussion: Sarah Kucenas and Alex Schier

Tues 6 Aug

8:30 – 9:30: Pit Talk: Optical Manipulation: Sarah Kucenas and Debbie Yelon

9:30 – 12:30: Lab Rotations: Photoconversion: Yelon, Ablation/Axotomy: Kucenas

12:30 – 1:30: Lunch

1:30 – 3:45: Lab Rotations: Ablation/Axotomy: Kucenas AND Fish facility tours with aquaculture vendors

3:45 – 4:15: Tea time with aquaculture vendors

4:15 – 6:00: Workshop: Zebrafish Husbandry and Breeding: April Freeman

7:30 – 9:00: Research Lectures & Discussion: Debbie Yelon and Tom Schilling

9:30 – 11:00: Cardiac regeneration results and terminal imaging: Poss and Yelon

Wed 7 Aug

8:30 – 11:00: In vitro fertilization: April Freeman and Debbie Yelon (Loeb 260)  AND Follow up on Ablation/Axotomy or Photoconversion: Kucenas and Yelon

11:00 – 12:00: Workshop: When should I (not) use zebrafish as a model system?: Alex Schier

12:00 – 1:30: Faculty (Alex Schier and Sarah Kucenas) lunch with students

1:30 – 3:30: Five-minute student presentations of favorite data

3:30 – 5:00: Course Wrap-up: discussion with Schilling and Yelon

5:00 – 5:15: Course Photo #1; wear course t-shirt

6:00 – 10:00: Course Dinner and Student Awards with Course Photo #2 at 6:30 pm

Thurs 8 Aug

Breakfast, room checkout and departure