Transgenic Laevis
Xla.Tg(CMV:Brainbow-1.0H)NXR
NXR_0001
Description: (Brainbow H) Default state is ubiquitous whole cell red fluorescence. Following Cre induced recombination cells will have membrane yellow or membrane cyan fluorescence.
Reference: Nature. 2007 Nov;450:56-62
NXR_0002
Description: (BrainbowL) Default state is ubiquitous whole cell red fluorescence. Following Cre induced recombination cells will have whole cell yellow or whole cell cyan fluorescence.
Reference: Nature. 2007 Nov;450:56-6
NXR_0003
Description: (C5) Whole embryo blue fluorescence; yellow fluorescence upon Cre or FLP recombination.
Reference: Nucleic Acids Res. 2003 Apr;31:e44 | Dev Dyn. 2006 Aug;235:2220-8
Xla.Tg(CAG:GFP)Mead
NXR_0004
Description: Fluorescence detectable in most tissues through development.
Available as frozen sperm, please allow 8 months for live animals.
Reference: Mead Lab
Xla.Tg(CAG:Venus)Ueno
NXR_0006
Description: (CAG-venus(YFP) LineB) pCAGGS driving Venus YFP expression under the control of the CAG promoter and is followed by the rabbit β-globin polyA signal sequence.
Reference: Nat Biotechnol. 2002 Jan;20:87-90 | Dev Dyn. 2005 Jun;233:562-9
Xla.Tg(actc1:GFP)Mohun
NXR_0007
Description: (Cardiac actin:GFP) 580bp of X. laevis actc1b gene driving GFP expression. Embryonic striated muscle including heart.
Reference: Dev Biol. 2002 May;5:57-70 | Development. 2003 Aug;130(16): 3865-76
Xla.Tg(CMV:GFP)Murray
NXR_0008
Description: (CMV-GFP) CMV promoter driving GFP expression, Whole embryo green fluorescence.
Reference:
Xla.Tg(CMV:GFP,dsRed)Amaya
NXR_0009
Description: Initial ubiquitous green fluorescence switches to red fluorescence following FLP recombinase activation. The constitutive simian cytomegalovirus CMV IE94 promoter/enhancer driving GFP flanked by FRT sites (for FLP site specific recombination). After FLP recombination, CMV drives expression of dsRed.
Previous name: Xla.Tg(CMV:GFP,RFP)Amaya
Available as frozen sperm, please allow 8 months for live animals.
Reference: Gene. 1996 Nov;180:225-7 | Nucleic Acids Res. 2001 Jun;29:E53-3 | Nucleic Acids Res. 2003 Apr;31:e44
Xla.Tg(Xtr.col2a1:GFP;cryga:GFP)Hank
NXR_0010
Description: (Col2a1-GFP) X. tropicalis collagen 2α1 promoter and first intron driving eGFP, γ-cry driving GFP marker. GFP expression in cartilaginous skeleton.
Reference: Int J Dev Biol. 2010;54(1):141-50 | Xenbase
Xla.Tg(eef1a1:GFP;cryga:GFP)Krieg
NXR_0011
Description: (Ef1α-GFP) Eef1a1 gene driving GFP expression, γ-cry driving GFP marker. Widespread GFP expression apparent from mid-gastrula on.
Available as frozen sperm, please allow 8 months for live animals.
Reference: Gene. 1994 Sep;147:223-6
Xla.Tg(CMV:memGFP,cryga:mCherry)NXR
NXR_0012
Description: (CMV:memGFP) CMV promoter driving GFP in cell membrane, γ-cry driving mCherry marker. Ubiquitous membrane GFP expression and mCherry in lens.
Reference: Custom transgenic generated by the NXR
Xla.Tg.(Rno.elas:GFP)Jmws
NXR_0013
Description: (Elas-GFP) Rat elastase promoter driving GFP. GFP expression in the pancreas.
Reference: Mech Dev. 1999 Nov;88:221-7 | Development. 2009 Jun;136:1791-800 | Dev Biol. 2007 Apr;304(2): 786-99 | Curr Biol. 2003 Jan;13(2):105-15 | Xenbase
Xla.Tg(CAG:KikGR)Flc
NXR_0014
Description: (CAG-KikumeGR) CAG promoter driving green-to-red photoconvertible fluorescent protein, Kikume Green-Red (KikGR)
Reference: BMC Development Biology 2009 Sept; 9:49 | Development. 2013;140:2409-2421
Xla.Tg(cryga:RFP;CMV:HyPer-YFP)Amaya
NXR_0016
Description: (HyPer) CMV promoter driving YFP of H2O2 indicator, HyPer. γ-cry driving RFP. Ubiquitous HyPer YFP expression, marker for integration selection; RFP expression in lens.
Reference: EXRC
Xla.Tg(Dre.noto:GFP)1Amaya
NXR_0017
Description: (FLH-GFP) Zebrafish flh promoter driving GFP in the pineal gland and posterior notochord.
Reference: EXRC | Concha, M., et. al. (2003) Local tissue interactions across the dorsal midline of the forebrain establish CNS laterality. Neuron; 39(3):423-38 | Xenbase
Xla.Tg(kdr:GFP)Mead
NXR_0018
Description: (FLK1-GFp) 2.5kb X. laevis flk-1 promoter driving GFP. GFP expression in vascular endothelial cells.
Reference: Mead Lab | Dev Dyn. 2007 Oct;236:2808-17 | Xenbase
Xla.Tg(gata1:GFP)Mead
NXR_0019
Description: (GATA1-GFP) GATA-1 promoter and first intron driving expression of GFP, sleeping beauty. GFP expression in red blood cells.
Reference: Mead Lab | Xenbase
Xla.Tg(cryga:GFP;hsp70-nog)Jmws
NXR_0020
Description: (HGEM-Noggin) Lenses glow green; heat-shock inducible Noggin.
Reference: Dev Cell. 2003 Sep;5:429-39 | Mech Dev. 2006 Sep;123:674-88 | Xenbase
Xla.Tg(hsp70:Xtr.dkk1;crya:GFP)Jmws
NXR_0021
Description: (HGEM-Xtdkk1) Heat-shock inducible Dkk1 to down-regulate Wnt/B-catenin signaling with a Gamma crystallin GFP selection marker.
Reference: Dev Biol. 2008 Apr 15;316(2):323-35 | Xenbase
Xla.Tg(slurp1l:GFP)Mohun
NXR_0025
Description: (LURP1-GFP) 5kb of X.laevis slurp1l gene driving eGFP. Embryonic macrophages, definitive myeloid blood cells, restricted neural expression, epidermis.
Reference: Mech Dev. 2002 Sep;117:173-86 | Dev Growth Differ. 2006 Oct;48:499-512 | Xenbase
Xla.Tg(myl3:eGFP)Mohun
NXR_0026
Description: 8kb of X. laevis myl3 gene driving enhanced GFP. GFP expression in heart mycoardium.
Previous names: MLC1V_GFP, mlc3:eGFP
Reference: Dev Dyn. 2005 Apr;232(4):1003-12 | Xenbase
Xla.Tg(myl2:eGFP)Mohun
NXR_0027
Description: (MLC2-GFP) 3kb of X. laevis myosin light chain promoter driving eGFP. eGFP expression in ventricular charmber, heart myocardium, and interhyoid facial muscle.
Reference: Development. 2004 Feb;131(3):669-79 | Xenbase
Xla.Tg.(nkx2-5:GFP)Mohun
NXR_0030
Description: (Nkx2.5-GFP) 8kb promoter of X. laevis nkx2-5 gene driving GFP expression. Early heart field, mesoderm, pharyngeal, endoderm, spleen.
Reference: Dev Biol. 2000 Nov 1;227(1):65-79 | Xenbase
Xla.Tg(hsp70:CActnnb1-GFP;Rno.elas:GFP)Jmws
NXR_0033
Description: (pHS-bCatenin-GFP:Elas-GFP) Generated via REMI (without restriction enzyme) with a cassette containing heatshock promoter driving expression of N-term truncated beta-catenin (stabilized CA form) tagged with GFP. Presumably the cassette also included elas:GFP but that is not described in the paper. The original vector from which pHsβcat∗GFP was derived was named pCH85.
Reference: Developmental Biology, 2008, Sept 15; 316(2): 323-335
Xla.Tg(smad3:eGFP;cryga:GFP)Mohun
NXR_0034
Description: (SMAD-GFP) 1.5kb of X. laevis Smad3 gene driving eGFP. Dorsal mesoderm post gastrulation, heart endocardium, widespread expression at larval stages.
Reference: Mech Dev. 2011 May-Jun;128:303-15 | Xenbase
Xla.Tg(tubb2b:mapt-GFP)Amaya
NXR_0035
Description: (NBT/Tubb2-GFP) 3481bp promoter and 5’UTR of tubb2b driving expression pf mapt-fusion. Green fluorescence in neaural tissue.
Reference: Xenbase
Xla.Tg(cryga:eGFP)Amaya
NXR_0036
Description: (xCry-GFP) The crystalline –GFP construct was generated by sub-cloning 2.2 kb a fragment of the crystalline genomic DNA encompassing the promoter region (received from Robert Grainger), upstream of an enhanced version of the GFP3.
Reference: Gene. 1993 Jun;128:189-95
Xla.Tg(CMV:eYFP,eCFP)1Ryff
NXR_0040
Description: (Y2) Whole embryo blue fluorescence; yellow fluorescence upon Cre recombination.
Reference: Nucleic Acids Res. 2003 Apr 15;31:e44
Xla.Tg(pax3:Katushka;cryga: Venus)EXRC
NXR_0049
Description: (Pax3-Katushka) 2916bp X. laevis Pax3 promoter driving expression of Katushka in the neural ectoderm (neural crest).
Reference: Pax3 promoter from Roberto Mayor (Mauricio Moreno) | Xenbase
Xla.Tg(Xtr.tbxt:Katushka;cryga:Venus)EXRC
NXR_0050
Description: (Brachyury-Katushka) 3856bp X. tropicalis Brachyury promoter driving expression of Katushka, γ-cry driving Venus marker. Katushka expression in muscles post-gastrulation and Venus in lens as integration marker.
Reference: EXRC
Xla.Tg(Xtr.cdknx:GFP)Papal
NXR_0051
Description: (Xic-GFP) 4.7 kb X. tropicalis p27 Xic1 promoter driving GFP expression in the neural tube and the eye.
Available as frozen sperm, please allow 8 months for live animals.
Reference: EXRC | Carruthersa, S., et. al. (2003) “Depletion of the cell-cycle inhibitor p27Xic1 impairs neuronal differentiation and increases the number of ElrCþ progenitor cells in Xenopus tropicalis.” Mech of Dev. 120; 607–616 | Xenbase
Xla.Tg(Rno.tuba1a:GFP)Brown
NXR_0053
Description: (TubulinA1-GFP) Rat tubulin a-1 promoter fused in grame to GFP. GFP expression in the nervous system and later in growing limbs.
Reference: EXRC | Marsh-Armstrong, N., et. al. (1999) “Germ-line transmission of transgenes in Xenopus laevis.” PNAS 96, 14389-14393 | Xenbase
Xla.Tg(ins:memGFP,cryga:mCherry)Brown
NXR_0056
Description: (Insulin-memGFP) Laevis 5kb insulin promoter driving memGFP expression; gamma crystallin mCherry selection
Reference: Transgenic generated by Donald Brown
Xla.Tg(CMV:eGFP-OMP25)Wtnbe
NXR_0059
Description: (Dria-GFP) CMV driving eGFP tagged OMP25 transmembrane segment. GFP expression of mitochondria membranes.
Reference: Dev Growth Differ. October 1, 2012; 54 (8): 767-76
Xla.Tg(tubb2b:GFP,Aequorin)NXR
NXR_0062
Description: (Tubb2-GFP-Aequorin)
Reference: Custom transgenic generated by the NXR for Andrew Miller
Xla.Tg(CMV:Aequorin,GFP)NXR
NXR_0063
Description: (CMV-Aequorin-GFP) Aequorin is a Ca2+ dependent bioluminescence photoprotein from hydrozoan A. Displays an emission peak at 470nm (blue light).
Reference: Custom transgenic generated by the NXR for Andrew Miller.
Xla.Tg(WntREs:dEGFP)Vlemx
NXR_0064
Description: (pbin7LEF-GFP/Wnt Reporter) Construct contains a synthetic Wnt-responsive promoter – consisting of 7 copies of a TCF/LEF1 binding DNA element, a minimal TATA box and a reporter gene encoding destabilized EGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence. GFP expression of dynamic patterns of Wnt/β-catenin signalling activity.
Reference: Proc Natl Acad Sci USA. 2010 Sep 14; 107(37): 16160-16165
Xla.Tg(rho:Eco.nfsA)Zuber
NXR_0067
Description: (XOPNTR) Rod optsin promoter driving E. coli nitroreductase.
Reference: FEBS Lett. 1998 Feb;423:117-21 | Invest Ophthalmol Vis Sci. 2011 Jan;52:364-73
Xla.Tg(Mmu.tal1:GFP)Patnt
NXR_0069
Description: (SCL-GFP) Mouse SCL +19 enhancer which contains a GATA-ETS-GATA core motif driving GFP. Expression in haematopoietic precursors.
Reference: EMBO J. 2002 Jun 17;21(12):3039-50 | Mol Cell Biol. 2010 Dec;30(24):5741-51 | Xenbase | EXRC
Xla.Tg(tuba1a:MyrPalm-mRFP1)NXR
NXR_0074
Description: (AlphaTubulin-UTR-RFP) tubulin alpha 1a promoter driving expression of membrane bound red fluorescent protein primarily in the ciliated epidermal cells.
Reference: Custom transgenic generated by the NXR for John Wallingford
Xla.Tg(CMV:vsFP_Butterfly1.2)NXR
NXR_0092
Description: (CMV-Butterfly VSFP1.2) VSFP is voltage sensitive fluorescence protein. VSFP-Butterfly 1.2 enables optical recordings at high spatial resolution with intrinsic assignment of recorded signals to VSFP-targeted (neural) cell populations.
Reference: Custom transgenic generated by the NXR for Mike Levin
Xla.Tg(CMV:hist2h2be-RFP;cryga:Venus)NXR
NXR_0094
Description: (CMV-H2BmRFP) CMV promoter driving RFP in nuclear membrane, γ-cry driving Venus marker. Ubiquitous nuclear RFP expression, and Venus expression in lens.
Reference: Custom transgenic generated by the NXR
Xla.Tg(CMV:vsFP2.42_SRL)NXR
NXR_0095
Description: (CMV-VSFP2.42-SRL) vsFP is a voltage sensitive Fluorescent Protein. vsFP enables optical recordings at high spatial resolution with an intrinsic assignment of recorded signals to vsFP-targeted (neural) cell populations.
Reference: Custom transgenic generated by the NXR for Mike Levin
Xla.Tg(CMV:cetn4-RFP)NXR
NXR_0100
Description: (Centrin-RFP) CMV promoter driving RFP and (cetn4) fused with RFP. RFP expression in the microtubules of cilia/ciliated cells.
Reference: Custom transgenic generated by the NXR for Brian Mitchell.
Xla.Tg(Dre.cdh17:eGFP)NXR
NXR_0102
Description: (Cdh17inteGFP) Zebrafish(Dre) Li-cadherin (CDH17) driving eGFP. GFP expression in the pronephros/pronephric tubes.
Reference: Custom transgenic generated by the NXR for Rachel Miller | Genes 2018, 9(4)
Xla.Tg(tuba1a:deup1-eGFP)NXR
NXR_0103
Description: (Deup1-GFP) Tubulin promoter driving Deup1 fused to eGFP. eGFP expression of deuterosomes and centrioles specifically in multi-ciliated cells. Marker for visualizing centrioles.
Reference: Custom transgenic generated by the NXR for Brian Mitchell | Xenbase
Xla.Tg(Mmu.prrx1:GFP3)Tamur
NXR_0106
Description: (mPrx1-GFP) GFP labels mesenchymal cells in limb bud and limb blastema.
Reference: EXRC | Dev Biol 304 (2007) 675–686 | Xenbase
Xla.Tg(tubb2b:GCaMP6s,Rno.elas:GFP)NXR
NXR_0107
Description: (ElasGFP:Tubb2-GCaMP6s) Neaural expression of the green Ca2+ sensor, GCaMP6s, and expression of GFP in the pancreas
Reference: Custom transgenic generated by the NXR
Xla.Tg(CMV:Hsa.EB3-eGFP)Ueno
NXR_0109
Description: (EB3-GFP) CMV driving eGFP of human EB3. Ubiquitous expression of eGFP in microtubule plus ends
Reference: EXRC | Takagi et al. 2013 Dev. Growth Differ 55, 422-433
Xla.Tg(CMV:Hsa.B4GALT1-eGFP)Ueno
NXR_0110
Description: (GFP-Golgi) CMV driving GFP of hb4GalT transgene. Ubiquitous expression of eGFP in the golgi appartus.
Reference: EXRC. Takagi et al. 2013 Dev. Growth Differ 55, 422-433.
Xla.Tg(hsp70:eYFP)Ryff
NXR_00111
Description: (HSP Cre1(C5)) Cross of HSP:Cre1 and C5, heat shocked in embryogensis and EYFP positive tadpoles were grown.
Reference: EXRC
Xla.Tg(CMV:calr-RFP)Ueno
NXR_0113
Description: (RFP-ER) CMV promoter driving RFP in CALR targeting sequence. Ubiquitous expression of RFP in the endoplasmic reticulum.
Reference: EXRC | Takagi et al. 2013 Dev. Growth Differ 55, 422-433 | Xenbase
Xla.Tg(CMV:RFP-CAAX)Ueno
NXR_0115
Description: (RFP-Mem) CMV promoter driving RFP in cell membrane. Ubiquitous membrane RFP expression.
Reference: EXRC | Takagi et al. 2013 Dev. Growth Differ 55, 422-433
Xla.Tg(Dre.mpeg1:eGFP;cryga:mCherry)Amaya
NXR_0118
Description: (mpeg-EGFP,crygamCherry) Zebrafish mpeg promoter driving GFP, γ-cry driving mCherry marker. GFP expression in the macrophages and mCherry in the lens.
Reference: EXRC | Ellett, F. et. al. (2011) “Mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish”. Blood; 117(4):e49-56
Xla.Tg(cryga:mCherry;CMV:6xMyc-EosFP)NXR
NXR_0119
Description: (CMV:EosFP)
Reference: Custom transgenic line generated by the NXR for Michael Levin
Xla.Tg(hsp70:Cre)Ryff
NXR_0121
Description: (Hsp:Cre1) Cre recombinase drive by HSP70
Reference: EXRC | Roose,M., et. al. (2009) “Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis.” Trans Res. 18, 595-605
Xla.Tg(rho:eGFP-atp1a3)Baker
NXR_0122
Description: (eGFP-ATP1a3) Xenopus rhodopsin promoter driving expression of eGFP tagged neuronal α3 isoform of the Na+/K+-ATPase in the rod photoreceptors.
Referenece: Traffic. 2015 Dec;16(12):1239-53 | Xenbase
Xla.Tg(Hsa.UBC:Gal4-VP16;cryga:CFP;UAS:mCherry-msn;UAS:Venus-mapt)Amaya
NXR_0123
Description: (Ubi-Gal4:UAS-venusTau:UAS-mCherryMoe)
Ubiquitous red fluorescence marking F-actin and green fluorescence marking microtubules. Cyan fluorescence marking the eye lens. Line was generated by digestion of three plasmids with Asp718 and then co-injection via REMI. First plasmid: 1.5 kb of human ubiquitin C promoter driving expression of Gal4-VP16 and gamma crystalin promoter driving expression of CFP. Second plasmid: UAS driving expression of mCherry tagged moesin. Third plasmid: UAS driving expression of Venus tagged microtubule associated protein tau.
Reference: EXRC
Xla.Tg(cryga:mCherry;CMV:6xHis-CaMPARI)NXR
NXR_0124
Description: (CMV:CaMPARI)
Reference: Custom transgenic line generated by the NXR for Michael Levin
Xla.Tg(tubb2b:ChIEF,Rno.elas:GFP)NXR
NXR_0125
Description: (Elas:GFP,Tubb2bChIEF) Neaural expression of blue light photoinducible channelrhodopsin, ChIEF, and expression of GFP in the pancreas.
Reference: Custom transgenic generated by the NXR
Xla.Tg(hsp70:DNyap1-GFP,cryga:tdTomato)Tamur
NXR_0126
Description: (HSP70:dnYAP-2A-GFP,gCry:tdTomato) 330 bp of promoter of the X. laevis hsp70 gene driving the expression dominant negative form of Yap (dnYap) tagged with GFP via 2A peptrides of 2.2 kb gamma crystalline promoter driving tdTomato.
Reference: EXRC
Xla.Tg(Hsa.UBC-Gal4;UAS:HyPer-YFP)Amaya
NXR_0127
Description: Detects hydrogen peroxide in vivo. Ubiquitous expression of YFP in tissues with reactive oxygen species (ROS).
Reference: EXRC
Xla.Tg.(hsp70:DNfgfr-Venus:cryga:RFP)Amaya
NXR_0128
Description: (HSP-XFDvenus; cryRFP) HSP70 promoter driving dominant negative FGFR with crystallin-RFP.
Reference: EXRC
Xla.Tg(Hsa.UBC:COX8A-dsRed;cryga:GFP)EXRC
NXR_0129
Description: (Ubic dsRed-Mito) 1.5kb of UbiC promoter driving dsRed and COX8, γ-cry driving Venus marker. Ubquitous dsRed expression in mitochondria, and Venus in lens.
Reference: EXRC.
Xla.Tg(CMV:H3K9ac-eGFP)Ueno
NXR_0130
Description: (Mintbody/CMV-H3K9ac-EGFP) CMV promoter driving eGFP of h3k9ac transgene. Ubiquitous GFP histone expression.
Reference: Ueno | Genes Cells. 2016 21:358–369 | Sci Rep. 2013; 3:2436
Xla.Tg(pax8:GFP)Ogino
NXR_0132
Description: (Pax8-GFP) Pax8 driving GFP. GFP expression of kidney and head.
Reference: Nat Commun. 2012 May 22;3:848 | Xenbase
Xla.Tg(CAG:SCAT3)Ueno
NXR_0134
Description: (NIBB6/Scat3) CAG-seCFP-Venus (an enhanced yellow fluorescent protein with efficient maturation property), containing the fluorescence indicator molecule SCAT3 and the rabbit β-globin polyA signal sequence, under control of the CAG promoter. SCAT3 is a hybrid protein of seCFP-Venus linked with the caspase-3 recognition sequence (DEVD) in pCAGGS.
Reference: Ueno Lab | Kominami, K. et al., 2006 Genes Cells 11:701-711 | Takagi C. et al. 2013 Dev. Growth Differ 55, 422-433
Xla.Tg(Rno.elas:rtTA;TRE:notchICD;cryga:GFP)Brown
NXR_0135
Description: (Elas-rtTA25-M2/tet(O)-NICD,cryGFP) Tetracycline inducible activated Notch expression in the exocrine pancreas.
Reference: Dev. Biology. 2011 351:2, 311-317
Xla.Tg(CMV:hist2h2be-GFP;CMV:mRFP)Flc
NXR_0139
Description: (CMV:H2B-GFP;CMV:mRFP) Cross of CMV:mRFP parent created CMV IE94 promoter in pCS2+ backbone, RFP inserted at BstBI and linearized with NotI; and CMV:H2B-GFP parent. Ubiquitous GFP expression of nuclear membrane and ubiquitous RFP expression of cell membrane.
Reference: Conlon Lab | H2B Xenbase