CRISPR/Cas9 Genome Editing: A Hands-on Experience

 

Wednesday

Thursday

Friday

Saturday

7:00

 

Breakfast 7 - 8:30 am

Breakfast 7 - 8:00 am

Breakfast 7 - 8:00 am

7:30

 

8:00

 

8:30

 

IT Session - Meigs:  Access to network and shared drives

Check embryos
8:00 - 8:30 am

Check embryos
8:00 - 8:30 am

9:00

 

Lab - Loeb 207:  Injections of gRNAs and dyes,
9:00 am - 11:30 pm

Lab - Loeb 207: Injections, Score Phenotypes, dechorinate, fix  and do phalloidin and dapi stain
9:00 am - 12:00 pm

Lab - Loeb 207: score second round of phenotypes, wrap up discussion about phenotypes and CRISPR,  discussion on ethics and CRISPR. Archiv's tour and Gift shop time
9:00 am - 12:00 pm

9:30

 

10:00

Arrival & Check-In:  10:00 am - 12:00 pm

10:30

11:00

11:30

Tour - MRC tank room
11:30 am - 12:00 pm

12:00

Lunch Noon - 1:00

Lunch - Noon to 1:30 pm

Lunch - Noon to 1:30 pm

Lunch - Noon to 1:30 pm

12:30

1:00

MBL Orientation & Safety Training
Loeb 263

1:30

Lab - Loeb 207: Intro to Imaging competition.     Intro to fluorescent imaging, GFP Lines, Fluorescent Staining, maybe hair cell staining  & Time Lapse Development
1:30 - 5:00 pm

Lab - Loeb 207: Image fixed, stained animals
1:30 - 5:00 pm

Departure & Check-Out: 1:30 pm

2:00

Lecture - Loeb 263: Introduction to embryology & development & overview of how CRISPR works

2:30

 

3:00

Lab - Loeb 207: Introduction to Microscopes and learn how to inject

3:30

 

4:00

4:30

5:00

Dinner 5:30 to 6:30 pm

Pizza & Movie Night beginning at 6 pm, Meigs Room, Swope Center

Dinner 5:30 to 6:30 pm

 

5:30

6:00

6:30

 

Work on images for image competition, submit by 10pm

 

7:00

7:30