Immunohistochemistry and Immunofluorescence (IHCIF)
This course is for graduate students, postdocs, research technicians and practitioners requiring hands-on wet lab training and the foundational knowledge required to design, validate and trouble shoot IHC and IF experiments in their labs.
Director: Kate Tubbesing, Neural Stem Cell Institute
Course Description:
This course is for graduate students, postdocs, research technicians and practitioners requiring hands-on wet lab training and the foundational knowledge required to design, validate and trouble shoot IHC and IF experiments in their labs. Our experienced instructors have taught this material to students from various scientific training backgrounds. The student to faculty ratio is designed to give opportunities for participants to ask questions that are specific to their research projects.
Lectures will focus on the fundamental knowledge which is re-enforced in labs and small discussions. Labs give students the opportunity to perform chromogen staining and immunofluorescent staining on provided specimens. Everyone will have opportunities to image their stained IF and IHC samples with a variety of microscopes available. There are opportunities for motivated students to spend more time on imaging, with supervised use of confocal and advanced imaging systems.
This is our first year staining sectioned organoids in addition to tissue sections and has provided an opportunity to have exciting discussions on how pathology can inform organoid modeling.
The IHCIF Course began in 2008 and moved to Woods Hole in 2010. Post pandemic, the course was held at Biotrac and Montgomery College facilities in Maryland for several cycles. We are now pleased to return to Woods Hole and partner with MBL once again. Please join us there!