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Back to Biology of the Inner Ear: Experimental and Analytical Approaches
BIE Protocols
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  • Biology of the Inner Ear: Experimental and Analytical Approaches

BIE Protocols

Many of the protocols used on the BIE course have recently been compiled into a book in the Neuromethods series from Springer Protocols. Details of the book can be found here and available for download through your home institution.

  • In situ hybridization
    • RNAscope (Walters – 2019)
  • Electrophysiology (Ashmore – 2009)
    • Frog Saccule Electrophysiology (Eatock 2011)
  • Immunohistochemistry
    • General Immuno Protocol
    • General Double-label Immuno Protocol
    • BrdU HCl-free, with nuclease protocol
    • EdU Immuno Protocol
    • Math1 Immuno Protocol
    • Myo6 Immuno Protocol
    • Sox2 Immuno Protocol
  • BrdU Protocol (Warchol – 2011)
  • Inner Ear Dissections
    • Dissection of Mouse utricle (Geleoc – 2011)
    • Bullfrog Sacculus Dissection (Cyr)
    • Chick Cochlea Dissection (Corwin)
    • Chick Dissection (Fekete)
    • Chick Utricle Dissection (Corwin)
    • Dissection Instruments (Corwin)
    • Mouse Dissection (Kelley)
    • Mouse Utricle Dissection (Corwin)
    • Mouse Utricle and Organ of Corti (Holt-Geleoc)
    • SYLGARD Dishes (Corwin)
  • Inner Ear Explant Cultures (Kelley)
  • Inner Ear Gene Transfection
    • Mouse Cochlea Electroporation (Kelley – 2009)
    • Mouse in utero Electroporation (Brigande – 2009)
  • Inner Ear Paintfills
    • Inner Ear Paintfill (Wu – 2009)
  • SEM (Forge)
    • SEM sample prep (Walters – 2019)
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