BIE Protocols

Many of the protocols used on the BIE course have recently been compiled into a book in the Neuromethods series from Springer Protocols. Details of the book can be found here and available for download through your home institution.

• In situ hybridization
  • RNAscope (Walters – 2019)

• Electrophysiology (Ashmore – 2009)
  • Frog Saccule Electrophysiology (Eatock 2011)
• Immunohistochemistry
  • General Immuno Protocol
  • General Double-label Immuno Protocol
  • BrdU HCl-free, with nuclease protocol
  • EdU Immuno Protocol
  • Math1 Immuno Protocol
  • Myo6 Immuno Protocol
  • Sox2 Immuno Protocol
• BrdU Protocol (Warchol – 2011)
• Inner Ear Dissections
  • Dissection of Mouse utricle (Geleoc – 2011)
  • Bullfrog Sacculus Dissection (Cyr)
  • Chick Cochlea Dissection (Corwin)
  • Chick Dissection (Fekete)
  • Chick Utricle Dissection (Corwin)
  • Dissection Instruments (Corwin)
  • Mouse Dissection (Kelley)
  • Mouse Utricle Dissection (Corwin)
  • Mouse Utricle and Organ of Corti (Holt-Geleoc)
  • SYLGARD Dishes (Corwin)
• Inner Ear Explant Cultures (Kelley)
• Inner Ear Gene Transfection
  • Mouse Cochlea Electroporation (Kelley – 2009)
  • Mouse in utero Electroporation (Brigande – 2009)
• Inner Ear Paintfills
  • Inner Ear Paintfill (Wu – 2009)
• SEM (Forge)
  • SEM sample prep (Walters – 2019)