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Mitosis in pollen mother cell of Easter Lily, observed with Shinya Scope-2. 16mm move time-lapsed ca. 450x. This historic sequence (1951) finally proved the reality of spindle fibers and fibrils [microtubules] which move chromosomes and define the plane of cell division in living cells.
Acrosomal reaction of Thyone sperm. Video-enhanced DIC in real time. Scale bar 10um. [Tilney and Inoue (1982) J. Cell Biol.93:820-827]
Cold-induced reversible loss of birefringence (i.e., depolymerization, or shift in dynamic equilibrium, of spindle microtubules) in developing sea urchin egg. Inoué (1952); Fuseler & Inoué (1975)
The movie shows several sequences showing changes in birefringence in oocytes of the marine annelid Chaetopteris that had formed meiotic spindles before exposure to centrifugal force in the Centrifuge Polarizing Microscope developed by S. Inoue. Spindles appear dark or bright according to their directionality with respect to that of the polarized light.
See S. Inoue et al. 2001. Centrifuge polarizing microscope II. Sample biological applications. J Microsc 201:357-367.
See S. Inoue et al. 2001. Centrifuge polarizing microscope II. Sample biological applications. J Microsc 201:357-367.