Optical Microscopy & Imaging in the Biomedical Sciences

Confocal (right panel) and STED (left panel) microscope images of U2OS cells labelled for TOMM20 protein (magenta) and DsDNA (green). Images acquired at OMIBS 2019 by Krishna C. Mudumbi (Yale University); sample prepared by Mark Lessard (Yale University). (Abberior STEDYCON system)
Course Information

Watch OMIBS WebinarCourse Date: August 19 – August 29, 2021

Deadline: May 13, 2021

2021 Course Schedule (PDF)

Directors: Joerg Bewersdorf, Yale University; and Alison North, The Rockefeller University

How To Apply

Applications are now closed.

Course Description

This course is designed primarily for research scientists, postdoctoral trainees, core facility directors/staff and graduate students working in the biological sciences. Biologists and physicists alike seeking a comprehensive introduction to microscopy and digital imaging will benefit greatly from the course. This 9-day course is limited to 24 students to ensure a truly interactive, hands-on experience. It consists of interrelated lectures, laboratory exercises, demonstrations, and discussions that will enable the participants to obtain and interpret high quality microscope data, to understand and assess potential artifacts, to perform quantitative optical measurements, and to generate digital images for documentation and analysis that accurately present the data. The course also places a strong emphasis on appropriate sample preparation, including choice of fluorescent probes and fluorescent proteins, and tissue clearing and refractive index matching. Particular emphasis will be placed on ‘picking the right tool for the job’.

Topics to be covered include:

  • Fundamental principles of microscope design, image formation, resolution and contrast;
  • Transmitted light and fluorescence microscopy techniques;
  • Cameras, signal to noise ratio, digital image recording, processing and analysis, multispectral imaging;
  • Advanced fluorescence – fluorescent probes, fluorescent biosensors, TIRF, FRET, FLIM, FRAP, polarization of fluorescence, fluorescence correlation spectroscopy;
  • Digital image restoration/deconvolution, and 3-D imaging principles;
  • Confocal and multiphoton laser scanning microscopy and light-sheet microscopy;
  • Super-resolution techniques including localization microscopy, stimulated emission depletion microscopy (STED), and structured illumination microscopy.

Students will have direct hands-on experience with state-of-the-art microscopes, a variety of digital cameras, and image processing software provided by major optical, electronics, and software companies. Instruction will be provided by experienced staff from universities and industry. Students are encouraged to bring their own, fixed biological specimens, and to discuss individual research problems with the faculty.

Financial Information: Tuition: $3,700.00. Room & Board: $810.00* (*Actual costs may be greater based on number of nights of occupancy). Some limited financial assistance may be available.

This course has been approved by The Histochemical Society for HCS Travel Awards in Imaging and Histochemistry. Further information is available at https://www.histochemicalsociety.org/travel-awards.

2020 Course Faculty & Lecturers

Allen, John (MBL/Vendor faculty)
Bewersdorf, Joerg (Yale University)
Day, Richard (Indiana University)
di Domenico, Angelique (New York Stem Cell Foundation)
Ferrand, Leanna (Vendor Faculty)
Fullerton, Stephanie (Vendor faculty)
King, Sharon (St. Jude Children’s Research Hospital)
La Riviere, Patrick (University of Chicago)
Lavis, Luke (Janelia Research Campus)
Legant, Wesley (UNC Chapel Hill)
Lessard, Mark (Yale University)
Leung, Jacqueline (Rocky Mountain Laboratories, NIAID/NIH)
McCall, Andrew (SUNY Buffalo)
McIlvain, Jim (MBL/Vendor faculty)
North, Alison (The Rockefeller University)
O’Shea, Clodagh (Salk Institute)
Palmer, Amy (University of Colorado, Boulder)
Rego, Hesper (Yale University)
Richardson, Douglas (Harvard University)
Shaw, Sidney (Indiana University)

Course Support

We are grateful to the following companies for their loyal and enthusiastic support of the OMIBS course:

Major support: Hamamatsu, GE Healthcare, Leica, Nikon, PCO, Zeiss

Additional support: Abbelight, Abberior, Andor, Arivis, AVR Optics, Axiom Optics, BioVision, Bitplane, Bruker, Cairn, Caliber ID, Chroma, Coherent, Double Helix, DRVision, Ibidi, ISS, MatTek, Mizar, Thermofisher, Thorlabs, TomoCube, VisiTech, 89North.