Zebrafish Development and Genetics

Course Information

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Course Date: Not offered in 2021

Course Website

2020 Schedule

Directors: Sharon Amacher, The Ohio State University; and Deborah Yelon, University of California/San Diego

Course Description

Over the past 20 years, the zebrafish has emerged as a powerful model system for the study of vertebrate development and disease. This intensive two-week course for advanced graduate students, postdoctoral fellows, and independent investigators will focus on the development and genetics of zebrafish. The course will cover time proven as well as novel technologies geared towards their application in zebrafish. Mornings and afternoons will be devoted mainly to laboratory exercises and the evenings to lectures and discussion. Limited to 22 students.

Lectures and labs in the first week will introduce students to early development of the zebrafish and to methods for manipulating and studying gene function, including genetic and small molecule screening, mRNA overexpression, optogenetics, and functional knockdown approaches. In addition, students will gain experience in relevant bioinformatics tools, cell fate mapping, regeneration experiments, and mounting and imaging of antibody and in situ samples for publication ready documentation. In the second week, students will be introduced to imaging of live cells and intracellular signaling events, biophysical manipulations, cell transplantation/chimera analysis, and behavioral testing of live animals. Informal ‘roundtable’ discussions held periodically during the course include topics such as reverse genetics, transgenesis, and zebrafish breeding and husbandry.

Each laboratory exercise will be under the supervision of a senior faculty member who will be assisted by one or two junior faculty members. Senior faculty will give research lectures about their work, in addition to leading discussions on topics covered in the course.

Financial Information: Tuition: $3,300; Room & Board: $1,078. The admissions process at the Marine Biological Laboratory is need-blind, meaning that we evaluate students on their merits alone, without weighing their financial situations. Financial assistance will be considered for those admitted students who are in need. Upon acceptance, students will be asked to complete a financial aid request form if they need assistance.

In 2019, 100% of those students in the Zebrafish Development and Genetics course who requested financial aid received some support. The amount of financial aid available from the MBL varies by course based on funding from grants and scholarships, but typically covers 70-100% of student need.

2020 Course Faculty & Lecturers

Sharon Amacher (Ohio State University)
Clare Buckley (University of Cambridge)
Rebecca Burdine (Princeton University)
Elisabeth Busch-Nentwich (University of Cambridge)
Andres Collazo (Caltech)
Jim Fadool (Florida State University)
Antonio Giraldez (Yale University)
Michael Granato (University of Pennsylvania)
Corinne Houart (Kings College London)
Anna Huttenlocher (University of Wisconsin, Madison)
Jan Huisken (Morgridge Institute for Research)
Sarah Kucenas (University of Virginia)
Mary Mullins (University of Pennsylvania)
Andrea Pauli (Institute of Molecular Pathology, Vienna)
Kenneth Poss (Duke University)
Dave Raible (University of Washington)
Alex Schier (Biozentrum, University of Basel)
Tom Schilling (UC Irvine)
David Schoppik (NYU School of Medicine)
Lila Solnica-Krezel (Washington University)
Deborah Yelon (University of California, San Diego)

Course Support

This course is supported with funds and equipment provided by:

Aquatic Enterprises
Howard Hughes Medical Institute
National Institute of Neurological Disorders and Stroke
National Institute on Deafness and Other Communication Disorders
National Institute of Mental Health
National Institute on Drug Abuse
National Institute of Child Health and Human Development
Sutter Instrument